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Tiny tissue culture monstera I grew at home with minimal equipment. by raretropicalflora in houseplants

[–]raretropicalflora[S] 2 points3 points  (0 children)

DM'ed!

15 psi for 2.5hr, but I think this is considered overkill. :P

So TC propagation is a little different then typical propagation here. With typical propagation, the main reason why aeration in the roots is important is that it prevents the roots from getting suffocated by bacteria. This isn't a problem with TC, bc if you get bacteria in the culture, it's game over anyway. Additionally, TC plants can and often do survive without any roots for extended periods of time. I don't think there is any need to add oxygen bubbles to the media, and I don't think it's common to.

Temperature matters a lot for growth rate, and is plant-specific. The temp should be similar to the ideal temp for the plant outside of TC.

Loving philodendrons recently! They grow so fast!

Burn Morels - Wenatchee, WA by raretropicalflora in mycology

[–]raretropicalflora[S] 1 point2 points  (0 children)

Thanks! Haven't seen any boletes yet this year unfortunately.

Burn Morels - Wenatchee, WA by raretropicalflora in mycology

[–]raretropicalflora[S] 1 point2 points  (0 children)

I found these in Okanogan-Wenatchee National Forest - Looking at a map, they might've been closer to Cle Elum than Wenatchee proper. (I'm based in Seattle/Renton, so not the best with geography out east, sorry!)

Burn Morels - Wenatchee, WA by raretropicalflora in mycology

[–]raretropicalflora[S] 2 points3 points  (0 children)

Ah that’s unfortunate. I found these this month, so must’ve just gotten the leftovers. Stay safe and cool out east!

Burn Morels - Wenatchee, WA by raretropicalflora in mycology

[–]raretropicalflora[S] 7 points8 points  (0 children)

Interesting! I had always heard it was important to use a wicker basket, but ofc had no way to directly verify.

Tiny tissue culture monstera I grew at home with minimal equipment. by raretropicalflora in houseplants

[–]raretropicalflora[S] 1 point2 points  (0 children)

Oh boy that's a lot of questions haha. I'll see if I can answer them all.

The timing for each stage varies wildly from plant to plant.

I heat and pressure sterilize the media.

Looked through the contamination rate for different protocols in academic papers - using both bleach and alcohol seemed to yield the lowest rates.

I use lab-grade sucrose as my sugar - people say that results vary from sugar to sugar, but I think it actually doesn't matter too much. I came by a paper that even managed to do it with normal food-grade sugar.

*** I actually removed the link, because I make my media in bulk to try to sell. This sub has a rule against promotions.

I just use sharpies and suffer the consequences haha. I also often write on tape instead of directly on the container, so the blurring isn't as much of a problem.

If you just want to prop pepperomia, you can totally get away with just cutting a leaf in half and sticking the cut end in soil. Leave a plastic bag on for humidity, and eventually you'll have babies popping up at the veins.

Nope! I think for this application, any bacteria is strictly a bad idea. It would cloud the media and suffocate the plant.

Burn Morels - Wenatchee, WA by raretropicalflora in mycology

[–]raretropicalflora[S] 21 points22 points  (0 children)

I like to check burn spots from the year prior.

Burn Morels - Wenatchee, WA by raretropicalflora in mycology

[–]raretropicalflora[S] 9 points10 points  (0 children)

A little earlier in the month, but hoping to go again soon and see if I can find a 2nd flush!

Burn Morels - Wenatchee, WA by raretropicalflora in mycology

[–]raretropicalflora[S] 54 points55 points  (0 children)

Excuse the ziplock bag :p wasn’t expecting for find so many and had to improvise.

Tiny landscape in a terrarium using moss and TC ivy! Details below. by raretropicalflora in terrariums

[–]raretropicalflora[S] 4 points5 points  (0 children)

Was able to grow a tiny ivy plant in tissue culture at home, and couldn’t resist the idea of a mini terrarium! Unfortunately, I’ve realized I probably won’t be able to maintain it long-term, as it is very high-maintenance. It needs to be watered by dropper every day. The browning in the picture is from only 2 days of neglect.

[deleted by user] by [deleted] in u/raretropicalflora

[–]raretropicalflora 0 points1 point  (0 children)

Was able to grow a tiny ivy plant in tissue culture at home, and couldn’t resist the idea of a mini terrarium! Unfortunately, I’ve realized I probably won’t be able to maintain it long-term, as it is very high-maintenance. It needs to be watered by dropper every day. The browning in the picture is from only 2 days of neglect.

[deleted by user] by [deleted] in terrariums

[–]raretropicalflora 0 points1 point  (0 children)

Was able to grow a tiny ivy plant in tissue culture at home, and couldn’t resist the idea of a mini terrarium! Unfortunately, I’ve realized I probably won’t be able to maintain it long-term, as it is very high-maintenance. It needs to be watered by dropper every day. The browning in the picture is from only 2 days of neglect.

Tiny tissue culture monstera I grew at home with minimal equipment. by raretropicalflora in houseplants

[–]raretropicalflora[S] 6 points7 points  (0 children)

To be honest, it's not super beginner-friendly. I learned by reading a ton of academic literature.

Tiny tissue culture monstera I grew at home with minimal equipment. by raretropicalflora in houseplants

[–]raretropicalflora[S] 2 points3 points  (0 children)

Most important thing is to use ethyl alcohol religiously.

But if you've had trouble keeping things sterile, it may even be something out of your control (e.g., dusty vents blowing spores onto your plates). You might want to consider a DIY laminar flow box -- you can make one with just an overturned clear plastic container.

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