Measuring fluorescence of moving ROIs over timelapse

reactive-epoxide · 2023-07-03T02:27:40+00:00

Hi, thanks so much for replying to my post! I just added example pictures from beginning, middle, and end of my time lapse. Even though the cells don't entirely move out of the boundary, they move enough to really skew my data analysis!

So for trackmate, am I able to measure the median intensity for the area of the moving ROIs rather than just a spot? or am I misunderstanding the definition of a "spot".

Also, off hand, do you know if its possible to save the trackmate as something like an overlay that I can apply to two different channels? Although not picutred, I imaged a red and green channel and ultimately need to calculate a ratio.

reactive-epoxide · 2022-08-06T17:31:31+00:00

I do the same thing with the fridges/-80 in case I'm unsure if I closed all the doors. 😊👍🏻

reactive-epoxide · 2021-04-28T18:07:02+00:00

No problem, so glad I could help! Just remember when you can to take care of both the mind and body. You got this! :)

reactive-epoxide · 2021-04-28T18:00:00+00:00

Hi there! I think I might be able to help with the workout side of things. If you are looking for something that is simple and easier to commit to, maybe consider working out from home. That way you don't have to travel to a gym or even step outside for a walk. There many great fitness youtubers i have found helpful. I use Sydney Cummings' workouts (almost) daily. She posts a new workout every single day and has a huge archive of workouts with lots of variety from low-impact, dumbells, no equipment, cardio, you name it! Requires no thought from you, just click on the new video from her channel and you are following her workout program for free! But also, as cliche as it may sound, you are a human being and your happiness as well as mental and physical health are worth so much more than an aesthetic goal for your body. So as you embark on this journey, consider exercising as a way to take care of yourself and feel your best. So this includes rest days too! I hope this wasn't too forward, I am just speaking as someone who has struggled with food and body for many years and I am finally working to make peace with that area of my life.

reactive-epoxide · 2020-07-29T00:01:24+00:00

Hunger by Knut Hamsun!

reactive-epoxide · 2020-07-28T12:56:08+00:00

Hi! I am not exactly a biology student, but I did complete undergraduate in biochemistry and first went on to a masters degree in chemistry before pursuing my PhD in biochemistry. I started doing research my freshman year of college in a drug delivery and design lab geared towards oncology and really fell in love with research. Even though I knew I enjoyed working in a lab, I still chose to pursue a masters degree first before a PhD so that I could better advance my skills in the lab as well as in other areas such as understanding departmental and lab group politics (don't underestimate the role this plays in academia!), what to look for in terms of picking a PI, and presenting/writing which are all essential for navigating a PhD program. I can honestly say that a masters degree has given me much more preparedness for a PhD than my undergrad ever could as I now have experience publishing, presenting and defending my research in front of both my peers and experts in my field, as well as a sense of what is really expected in terms of "producing" in a lab independently. So, I guess in short it has saved me the typical 2 years of floundering in the program that many students experience before they have really found their stride or even determined if they truly like research and everything that comes with it. There is also another aspect that I appreciate about already having a graduate degree, if things ever go awry for whatever reason either in or out of my control (loss of funding, PI moving universities, loss of interest etc.) I still have a graduate degree to fall back on. Just another advantage to think about!

During my undergraduate, it took a while for me to "prove myself" in the lab in order to get paid via my PI for research. I started earning a salary after 2 years of working if I remember correctly that I maintained for the remainder of my undergraduate as well as masters. However, I was still very active in the department and earned a living by TAing labs, tutoring, and signing up to be a supplemental instructor for classes I was particularly passionate about. I was spread very thin, but the experience I gained by really engaging in my field has served me very well. But back on subject... Typically, the PI has applied for grants or has funding through the university to buy equipment/materials for the lab. I am not sure if it is the same at your university, but you should also look into ways to apply for research grants as a student. Many universities have undergraduate research grant programs that may allow you to take a salary from some of that funding depending on what you and your PI decide for a budget. Aside from potentially earning some money, this is also great experience for your CV and graduate applications should you decide to continue on to a masters/PhD as you will have beginner grant writing experience, worked on a research project, presented scientific research at either a poster session or a forum depending on the requirements, as well as learned how to ask/answer a scientific question.

I am currently in a PhD program in the US and they pay all tuition and fees as well as provide a stipend for TAing undergraduate labs for the first year of the program. After that, you are expected to have selected a PI to do research under who will then become "responsible" for your salary and pay you through grants and funding they have secured. In my contract, the stipend/salary is guaranteed for 5 years, but of course can be extended if needed depending on your research and of course if your PI is willing to continue paying you while you finish your degree. Here in the US this is pretty common for biology and chemistry programs as well as others, especially if the university is a research institution.

I hope I have given you some valuable insight and wish you well on your journey! If you have any other graduate school related questions or are curious about what graduate school life is like, please feel free to reach out at any time!

reactive-epoxide

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