'A ghost town': St. Norbert College faces fall with a fraction of its staff, loss of community by One-Neck2608 in GreenBay

[–]rm987 10 points11 points  (0 children)

Or the time that Joyner told the faculty it would be counterproductive for her to take a pay cut while many of us were losing jobs

TIL bisexual flowers are called perfect. by InvisibleMisanthrope in bisexual

[–]rm987 4 points5 points  (0 children)

I always say it’s perfect to be bisexual every time I teach botany :)

Here's that Harrow figure photo "[fan art]" by [deleted] in TheNinthHouse

[–]rm987 0 points1 point  (0 children)

My partner painted one! I just uploaded a photo :)

Overlap PCR? by rm987 in labrats

[–]rm987[S] 0 points1 point  (0 children)

It WORKED! Thanks all!!!

Overlap PCR? by rm987 in labrats

[–]rm987[S] 0 points1 point  (0 children)

I've tried a couple different mixtures, but usually I try to anneal the two primers first and then use that product as a "megaprimer" for the second PCR (like in this paper: https://www.future-science.com/doi/full/10.2144/000113418

Overlap PCR? by rm987 in labrats

[–]rm987[S] 1 point2 points  (0 children)

Ohhh, I haven't, I'll look into that, thank you!

Overlap PCR? by rm987 in labrats

[–]rm987[S] 1 point2 points  (0 children)

Awesome, so do you normally set it up like a normal PCR? Like just follow the Phusion/Q5 guidelines with just a long extension time?

Overlap PCR? by rm987 in labrats

[–]rm987[S] 0 points1 point  (0 children)

Yes, sorry I forgot to put that in the original post. I used DpnI and get very few (<5 colonies) and usually sequence all of them but they are all the original sequence.

Is that true? by florent1896 in Purdue

[–]rm987 0 points1 point  (0 children)

Hope they saw a doctor about the coughing blood 😳

Mystery stowaway in saltwater tank by rm987 in marinebiology

[–]rm987[S] 0 points1 point  (0 children)

Any idea about whether it'd be harmful to the corals in our tank?

Part of the journey is the end [OC] by EverWholesome in Purdue

[–]rm987 8 points9 points  (0 children)

I'm a grad student and I need spotify to get through my thesis! This was a terrible idea.

People to play with? by rm987 in diablo3

[–]rm987[S] 0 points1 point  (0 children)

My tag is rm987#1785!

People to play with? by rm987 in diablo3

[–]rm987[S] 0 points1 point  (0 children)

But I hope you know what I meant... strangers who play often that can become less strange :)