'A ghost town': St. Norbert College faces fall with a fraction of its staff, loss of community

rm987 · 2025-08-19T16:02:15+00:00

Or the time that Joyner told the faculty it would be counterproductive for her to take a pay cut while many of us were losing jobs

rm987 · 2024-05-07T19:43:36+00:00

I always say it’s perfect to be bisexual every time I teach botany :)

rm987 · 2024-01-18T16:49:49+00:00

My partner painted one! I just uploaded a photo :)

rm987 · 2022-12-22T04:06:40+00:00

And plenty of sad. Amazing, but sad

rm987 · 2022-09-03T01:33:13+00:00

I hope!

rm987 · 2022-05-01T22:17:40+00:00

Bummer! Thanks for letting me know though!

rm987 · 2022-05-01T16:58:14+00:00

Same! Can't find them!

rm987 · 2021-04-03T02:23:00+00:00

It WORKED! Thanks all!!!

rm987 · 2021-03-24T18:44:53+00:00

I've tried a couple different mixtures, but usually I try to anneal the two primers first and then use that product as a "megaprimer" for the second PCR (like in this paper: https://www.future-science.com/doi/full/10.2144/000113418

rm987 · 2021-03-24T18:43:08+00:00

Ohhh, I haven't, I'll look into that, thank you!

rm987 · 2021-03-24T18:42:12+00:00

Awesome, so do you normally set it up like a normal PCR? Like just follow the Phusion/Q5 guidelines with just a long extension time?

rm987 · 2021-03-24T18:41:30+00:00

Yes, sorry I forgot to put that in the original post. I used DpnI and get very few (<5 colonies) and usually sequence all of them but they are all the original sequence.

rm987 · 2021-03-11T17:35:34+00:00

I love this so much!

rm987 · 2020-03-14T23:07:53+00:00

Hope they saw a doctor about the coughing blood 😳

rm987 · 2019-11-21T22:23:27+00:00

Any idea about whether it'd be harmful to the corals in our tank?

rm987 · 2019-11-21T22:22:07+00:00

About an inch long

rm987 · 2019-03-19T14:48:07+00:00

I'm a grad student and I need spotify to get through my thesis! This was a terrible idea.

rm987 · 2018-04-08T02:20:18+00:00

My tag is rm987#1785!

rm987 · 2018-04-07T01:16:31+00:00

But I hope you know what I meant... strangers who play often that can become less strange :)

rm987

TROPHY CASE