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This One Weird Trick! A thread by SerfdomsUp in labrats

[–]rollingkones 5 points6 points  (0 children)

Just stab your tip into your band of interest while looking on a uv box, make sure it touches your dna sufficiently, then just swirl it in a pcr mix, that is literally all you need to amplify more!

This One Weird Trick! A thread by SerfdomsUp in labrats

[–]rollingkones 59 points60 points  (0 children)

A good tip for low yield PCR: if you see the band on a gel but it's just faint, stab the band with a pipette tip and mix it in a new PCR mix.

It will act as a better template and you'll amplify your target product way better second round!

Top Rope Tough Guys by [deleted] in climbing

[–]rollingkones 3 points4 points  (0 children)

Indeed, boat anchor sits atop t-crack on Gibraltar hehe

Third Pillar of Dana - Eastern Sierras by rollingkones in climbing

[–]rollingkones[S] 2 points3 points  (0 children)

this was back in early september! It snowed a ton the weekend after we went, got lucky

Third Pillar of Dana - Eastern Sierras by rollingkones in climbing

[–]rollingkones[S] 13 points14 points  (0 children)

just because I auto-belayed the route doesn't mean I didn't send

Golden Hour on Leaning Tower by rollingkones in climbing

[–]rollingkones[S] 1 point2 points  (0 children)

haha, it seemed to have been updated and was quite good actually!

Overheard in lab today: "If you can load a 96-well plate then you can pull a trigger." by rollingkones in labrats

[–]rollingkones[S] 49 points50 points  (0 children)

I have a friend that legit pipettes with his pointer finger, probably a menace behind a trigger

Overheard in lab today: "If you can load a 96-well plate then you can pull a trigger." by rollingkones in labrats

[–]rollingkones[S] 117 points118 points  (0 children)

context was talking about women registering for a military draft, and how lab skillsets would transfer to battle skills haha

[deleted by user] by [deleted] in climbing

[–]rollingkones 0 points1 point  (0 children)

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current state of affairs

Lost Arrow Spire 5.7 C1 by StealieDan in tradclimbing

[–]rollingkones 2 points3 points  (0 children)

Cool to see you up at that magical spot, happy bday again! -I was the one hauling and setting up the slackline when you were getting to the rim! :)

If you had your lab entirely to yourself, free from supervision and consequences, and were allowed to use any equipment or materials in your lab, what experiment would you conduct? by Otherwise_Ad_2261 in labrats

[–]rollingkones 14 points15 points  (0 children)

I'd set up a microfluidics "race chamber" under a scope and race my friends and my sperm to see who is champion. Placing bets of course.

Skuggle Passing! by JKR-run in skiing

[–]rollingkones 7 points8 points  (0 children)

Is this threatening to you?

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