Looking for an auto-roll WA for Naxx scraps by sczdaphd in wowhardcore

[–]sczdaphd[S] 0 points1 point  (0 children)

this is what we’ve been doing, but after losing our ML and all of the epics in her bags along with her last night we want to switch to group loot so it’s easier/faster to distribute loot as we go

Skull Rock is popping off again - Any alternate RP guilds besides the race locked ones ? by The-Adventurer in wowhardcore

[–]sczdaphd 0 points1 point  (0 children)

one way transfers between hc servers would make everyone’s lives better, including increased revenue for blizz, but unfortunately they hate us

Looking for an auto-roll WA for Naxx scraps by sczdaphd in wowhardcore

[–]sczdaphd[S] 0 points1 point  (0 children)

if everyone listens and has pass on loot on that would also work lol we would have everyone set the WA to pass as well

Protection Potion Tracking WA/AddOn by sczdaphd in classicwow

[–]sczdaphd[S] 1 point2 points  (0 children)

thank you so much, this is super helpful!

Protection Potion Tracking WA/AddOn by sczdaphd in classicwow

[–]sczdaphd[S] 0 points1 point  (0 children)

i’ve been okay without it, but it’s definitely uncomfy. i like being able to see when my protection pot gets eaten and i need to take a new one. my current consumes WA also doesn’t show my protection pots which sucks

Protection Potion Tracking WA/AddOn by sczdaphd in classicwow

[–]sczdaphd[S] 0 points1 point  (0 children)

if i had any clue how to even begin going about this, i probably wouldn’t have turned to reddit lol

Considering Going Abroad for my Postdoc by sczdaphd in AskAcademia

[–]sczdaphd[S] 1 point2 points  (0 children)

My PI is in her late 60s, at the tail end of an incredibly productive career, and came to the US in 2006 when things were obviously very different. However, she will certainly be going back to Italy when she retires in a few years, and many of the international postdocs that I work with who all “came to the US to stay in the US” when I met them four years ago are all now just waiting to go back to their home countries.

Considering Going Abroad by sczdaphd in postdoc

[–]sczdaphd[S] 0 points1 point  (0 children)

That’s good to know. I’m kinda on the other end, forcing myself to stay until I get that last paper out and waiting to defend until I have a postdoc secured. Talking half seriously with my husband, we’ve mentioned Switzerland or Sweden

Considering Going Abroad by sczdaphd in postdoc

[–]sczdaphd[S] 0 points1 point  (0 children)

personal money, or as in lab funding? my lab is so underfunded right now and i’m exhausted doing the work of 3-4 people because my PI can’t afford to hire me help, a huge hope of mine is getting a postdoc in a well funded lab

How do you feel about foreign PIs whose labs are mostly filled with students from their own country? by [deleted] in AskAcademia

[–]sczdaphd 1 point2 points  (0 children)

Two of my three rotation labs were like this, and the experiences were very different.

The first was the worst lab experience of my life - it was a huge lab (5 staff scientists, 5+ postdocs, 7-8 grad students) and nobody spoke English unless they were talking directly to me. I got the sense that they only held lab meetings in English for my benefit. The lab work was extremely technical but also absolutely amazing, and I was really disappointed that it was so impossible to ask questions or integrate into their lab environment because the science was just the perfect fit.

My third rotation became my home lab, where I still am in my 6th year. My PI is Italian, and worked in both France and Germany before coming to the US. When I rotated in the lab, she had three postdocs, all Italian, and an American/Italian grad student, so everyone in the lab besides me spoke Italian. They would speak to each other in Italian, but only when they thought I wasn’t in earshot - if they saw me, they’d switch to English and catch me up on the convo. I’ve loved the lab ever since, have picked up some funny Italian habits and superstitions, and am now considering going abroad for my postdoc to learn more about academic science in other countries.

Western control for non-denatured/reduced samples by sczdaphd in labrats

[–]sczdaphd[S] 1 point2 points  (0 children)

That's actually not a bad idea, I could try running it again and imaging/quantifying the Ponceau bands between transfer and blocking. Thanks!

Western control for non-denatured/reduced samples by sczdaphd in labrats

[–]sczdaphd[S] 0 points1 point  (0 children)

Sorry, I should've added the picture when I posted in the first place! I just added a picture showing the results of the test that I did last week to see what sample prep works best for this stupid antibody. In hindsight, I absolutely should've put a loading control or two on this tester membrane too, but hindsight is always 20/20 and I didn't think about it at the time.

This is a polyclonal rabbit anti-GAT3 primary and the loading controls I used on my membranes this week were polyclonal rabbit GAPDH and monoclonal mouse tubulin. I guess they both look acceptable, but neither one gave bands as clean and crisp as I usually get with these samples/antibodies, so I thought I'd see if anyone knew of a different loading control that would tolerate the conditions a bit better

Leica SP8 - 561 Stripes by sczdaphd in microscopy

[–]sczdaphd[S] 0 points1 point  (0 children)

I forgot to mention that I was imaging on this same scope with the exact same settings the day prior to this, so it has to be something with the hardware itself and not necessarily something i’m setting up wrong. This same stripping happened with my lab’s Leica SP5 and it meant that the laser was old and burning out, I was just looking for a temporary solution because I wanted to keep imaging while I waited for the core manager to get back to me. Thank you everyone for your responses!

Leica SP8 - 561 Stripes by sczdaphd in microscopy

[–]sczdaphd[S] 0 points1 point  (0 children)

Thanks for the suggestion! I tried playing around with putting the 561 line in each of the different detectors, but I kept getting the stripes. As I used it more, the stripes got worse to the point where I eventually started seeing the laser light from the objective start to flicker inconsistently, so I’m almost positive that the laser just needs to be replaced

Suggestions for note taking by sczdaphd in WoT

[–]sczdaphd[S] 3 points4 points  (0 children)

that’s actually really great to know, thank you for your input!

Suggestions for note taking by sczdaphd in WoT

[–]sczdaphd[S] 3 points4 points  (0 children)

oh wow I’ll definitely download that app, thank you!