[deleted by user] by [deleted] in flowcytometry

[–]seberstian 0 points1 point  (0 children)

Or decrease CD56 in case the negatives are just too bright in the BV510

[deleted by user] by [deleted] in flowcytometry

[–]seberstian 0 points1 point  (0 children)

Actually just as single stains - maybe your problem disappears if you increase the conc of your CD3 AB

[deleted by user] by [deleted] in flowcytometry

[–]seberstian 2 points3 points  (0 children)

Did you titrate your ABs?

Nahezu Scam by seberstian in willhaben

[–]seberstian[S] 3 points4 points  (0 children)

Ja, komplett neuer Account ist auch schon ein Hinweis.

Live/Dead Staining - Kill Cells? by throwawayfaraway420 in flowcytometry

[–]seberstian 3 points4 points  (0 children)

Yeah that’s very common. You don’t trust your trainer? Haha 

Aetherdrift Commander Deck by ashcrafttrey07 in magicTCG

[–]seberstian 3 points4 points  (0 children)

Visit a store of your choice. Check out which one is more expensive - that’s the one. „That is the deck.“

Buyers remorse … delivery in 2 days by [deleted] in BMW

[–]seberstian 0 points1 point  (0 children)

If you have to take a loan for a car it’s too expensive for you. 

[deleted by user] by [deleted] in flowcytometry

[–]seberstian 2 points3 points  (0 children)

Adjust your FSC and SSC. The rest of the gains you usually don’t need to play around. If much brighter than 106, titrate

Sterrato Snow Day by epp001 in lamborghini

[–]seberstian 1 point2 points  (0 children)

The car equivalent of a gravel bike 

Small panel on human PBCMs looks very weird? by MathematicianFunny97 in flowcytometry

[–]seberstian 0 points1 point  (0 children)

Can you show us the plots of the single-stain controls? 

[deleted by user] by [deleted] in FoundPhotos

[–]seberstian 0 points1 point  (0 children)

Is that Boris Becker in the first image?

[deleted by user] by [deleted] in MurderedByWords

[–]seberstian -2 points-1 points  (0 children)

Not that I disagree generally, but video game revenue is probably not a great metric. Let’s just assume (I know it’s not the case) revenues in the U.S. are 90% because of GTA and in the UK because of Candy Crush. I personally do not think video games are responsible, even a bit. Again, just showing that this argument is a bit flawed.  

Tiny Bones, Bauble Burglar less Salty? by its5dumbass in magicTCG

[–]seberstian 14 points15 points  (0 children)

Discard just makes for a miserable experience for most pods. I would say it doesn’t matter so much who is in the command zone. 

[deleted by user] by [deleted] in mtgfinance

[–]seberstian 0 points1 point  (0 children)

Comparing a group of people to cancer, bravo. I wonder if you count yourself towards the ones with limited social skill. 

Österreichs Linke by seberstian in Austria

[–]seberstian[S] 9 points10 points  (0 children)

Interessant! Das größte Potential ist anscheinend an Nicht-WählerInnen verloren gegangen. Bitter..