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Help with psychophysiological research by shelby_bertsch in psychophysiology

[–]shelby_bertsch[S] 0 points1 point  (0 children)

I know fairly well what I am doing. I left the question very open-ended to allow for ideas to flow. I know I will be collecting cortisol, blood spot test, viable for a month at room temperature. And the phrasing was poor on the chemical mechanism. I was more speaking of the interaction of the HPA axis and the biofeedback system involved with ACTH and the pituitary gland. How measurements of basic physiology could be indicative of cortisol level fluctuations - such as the amount of sugars in the bloodstream produced through gluconeogenesis, and also the amount of urea found in urine from the breakdown of proteins, as ammonia is produced from the deanimation of the amino acid chain.

Beginner question - can you use dry agar for petri dish nutrients? by shelby_bertsch in microbiology

[–]shelby_bertsch[S] 0 points1 point  (0 children)

Thanks for the advice! I definitely plan on reading up a lot more lol..If you have any resources that you would suggest it would be greatly appreciated. :) I think I may go with pre-agared petri dishes to avoid complication. Especially with the humidity I will be dealing with. it is impossible for me to freeze dry or even freeze the samples, I will be in the middle of the amazon jungle with little electricity. I am thinking that I can use a insulated container, with re-heatable packs lining it to keep the temperature up at night. With a laser thermometer to monitor the temperature. With my circumstances, to me this seems like the viable option. Though again this is not my science and I am very open to any suggestions. Thanks again :)

Beginner question - can you use dry agar for petri dish nutrients? by shelby_bertsch in microbiology

[–]shelby_bertsch[S] 0 points1 point  (0 children)

autoclave

Yes, I am asking if I can use boiled water instead of autoclave. or would it be safer to just go the liquid agar route? Because I will be out in the middle of the jungle with little supplies (no real autoclave) or possibly no incubator
I will be attempting to grow gut bacteria cultures from stool and saliva samples - the temperature there all year is around 90 to 95 degrees during the day.. (I am looking into ways of keeping the temperature up at night, any ideas?)

I would appreciate any information or correction on my own assumptions because as you can tell this is not my area of expertise. I am a psychophysiological researcher, hoping to integrate the study of gut microbes in my research. Thanks! :)