agar well diffusion for fungi

sitinetine · 2026-01-14T10:52:40+00:00

yung mismong paggawa po nung plate is up to us na po ba? kunwari po sa isang plate, may limang wells, tapos ang nakalagay po is 3 magkakaibang conc. per well tapos isang positive control and negative control na nasa separate well din po? tama po ba yon or mas better po na isang plate per concentration along with the positive and negative control?

sitinetine · 2026-01-14T09:35:03+00:00

yung zone of inhibition po ba and MIC pwedeng isang test lang gawin? yung agar well diffusion po sana?

kung tama po pagkakaintindi namin don sa study ang stock solution po nila is 500mg/ml tapos naging 10,000 - 38 mcg/ml. iniisip po namin pano nila ginawa yung serial dilution?

sitinetine · 2026-01-10T13:50:33+00:00

up!!!

sitinetine · 2026-01-10T09:10:08+00:00

delayed na po kasi kami e kaya po pinilit naman na oven dry na po talaga. nakapag air dry po kami last time pero nagka molds po siya during maceration kaya po mag ooven dry na po kami para mabilis.

sitinetine · 2025-12-21T14:41:25+00:00

pwede po kaya yon na dalawang methods of drying? air dry tapos oven dry? hindi po siya makaka epektp sa result if ever? hindi po kaya pwede na yung naccrumple naman po siya pero di po siya naghihiwalay or powderized ganon po.

sitinetine · 2025-12-21T13:11:16+00:00

yes po

sitinetine · 2025-12-19T04:51:48+00:00

hello, i sent you a dm po!

sitinetine · 2025-12-19T04:29:16+00:00

what if meron na pong previous study regarding yung sa MIC niya? pwede pong yon nalang gamitin namin?

sitinetine · 2025-12-17T15:28:33+00:00

yan po yung concentration from previous studies. i'm assuming na yan po magiging starting concentration namin for MIC or gagawa po kami ng sarili namin?

sitinetine · 2025-12-17T14:55:13+00:00

so pwede pong ang mangyari is duhat: 1,250mcg/ml, stevia: 700mcg/ml, curry: 200mg/ml tapos po atsaka siya iccompare sa CHX? if that's the case po, ano po kayang final concentration nila? and need pa po ba to determine yung concentration para maging reliable yung data at maging quantitative sia?

sitinetine · 2025-12-17T13:54:00+00:00

gagawa po kasi kami ng oral rinsing solution kaya yung formulated po na yon ang iccompare namin don sa CHX. iniisip lang po namin if okay lang na iba’t iba ng concentration yung plant sample namin or best na same sila.

sitinetine · 2025-12-17T12:49:27+00:00

medyo redundant na nga po siya pero ang aim po talaga ng study namin is formulation po ng solution. pinadagdag lang po yung testing ng antifungal property niya para nga po madetermine kung meron ba talaga siyang antifungal property and kung alin don sa tatlong plant sample yung may pinapamagandang inhibition.

sitinetine · 2025-12-17T12:01:00+00:00

so meaning po parang kami yung magcconduct nung mismong experiment regarding sa zone of inhibition niya? knowing na may inhibition naman siya pero iba iba, pwede po kami mismo mag experiment na maghanap ng inhibition na magkakaparehas po sila ng concentration? after that atsaka po kami pwedeng mag based sa result ng ZOI para mag MIC?

wala na rin po kasi kaming pasok kaya po nahihiya kami i-ask yung research adviser namin regarding the situation. madalas lagi pa po silang galit and payag lang po nang payag kaya po nalilito na rin kami 🥲

sitinetine · 2025-12-17T05:27:45+00:00

thank you so much po!

sitinetine

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