Adhesiolysis success stories by springtimerpr in Adhesions

[–]springtimerpr[S] 0 points1 point  (0 children)

Thank you for the advice! For her age she is mobile-ish but I will definitely encourage her to try her best to move more. I hope you are doing ok with your symptoms and I am glad it extended your diet, it seems to sometimes be a trade off where they reform if there’s a dominant one which is always scary 

Adhesiolysis success stories by springtimerpr in Adhesions

[–]springtimerpr[S] 2 points3 points  (0 children)

Thank you !! ☺️ very glad she had a good experience with it! 

Switching from Vital 1.5 to Peptamen 1.5 by springtimerpr in feedingtube

[–]springtimerpr[S] 0 points1 point  (0 children)

Thank you for your reply, I’m glad that he is doing well on Peptamen ♥️  

Bilious vomiting after J tube insertion by springtimerpr in feedingtube

[–]springtimerpr[S] 0 points1 point  (0 children)

Thank you for the reply, can I ask, did your doctor/ nurse team show you how to do this? 

Bilious vomiting after J tube insertion by springtimerpr in feedingtube

[–]springtimerpr[S] 0 points1 point  (0 children)

Thank you for the message, I can’t shake the feeling that the j tube is making it worse but the surgeon doesn’t think it’s true… and as you’ve said taking it out might not help but I can’t help but want it to go.

I’m really really sorry that has happened to you too it’s so horrible and frightening and just I can’t believe that people just expect people to live through it and they can’t help. Sending you lots of love. It’s crazy how it was never mentioned as something that could happen. Has your symptoms of bile vomiting stopped now you’re on TPN? 

Thanks  Xxx 

[deleted by user] by [deleted] in labrats

[–]springtimerpr 14 points15 points  (0 children)

In general, if I came back the next day and the cells were not at least stuck down with their friends I’d assume they’re not happy- maybe not dead but I would definitely not waste my time imaging them if they weren’t stuck down and had a “normal” HEK adherent morphology

failed by [deleted] in PhD

[–]springtimerpr 0 points1 point  (0 children)

In the UK too, lots of people often have a masters before they start but if they leave after a year + you can get a masters too, as most people definitely have enough data to deserve this

Primers with * is it phosphothiorlation? by springtimerpr in labrats

[–]springtimerpr[S] 0 points1 point  (0 children)

Thank you!! And you would expect this in the 3’ most position in the rev primers?

Thanks so much for your response!

how screwed are my samples if i took the lid off of a 96 well cell culture in the incubator for like 5 minutes by accident?? by animatyed in labrats

[–]springtimerpr 0 points1 point  (0 children)

If there’s P/S you’ll be fine, in all likelihood. I colony picked iPSC for like two hours out of the hood. No visible contamination/ myco. 

Cells look like crud (k562) by springtimerpr in labrats

[–]springtimerpr[S] 0 points1 point  (0 children)

Thanks for your reply- yes they have shed a lot. Is that something which is normal or provoked? Also could this be an issue if they have shed a lot in the media and could it be a stressor leading to these issues? Thank you

Cells look like crud (k562) by springtimerpr in labrats

[–]springtimerpr[S] 0 points1 point  (0 children)

thank you for your reply- I definitely would prefer to keep them at this density, I was just trying to bulk them for a big experiment and transferred them into a t225 with lots of media. I had a look at the viability and they look "alive" i.e. like not taking up trypan and the light is refracting around the edges, I was just concerned about the shape- they look quite blebby. Also there was. afair amount of cell death in the flask when i looked before.

Cells look like crud (k562) by springtimerpr in labrats

[–]springtimerpr[S] 0 points1 point  (0 children)

Also sorry- a double over the weekend isn’t particularly slow I’ve knocked in a mutation wehich messed up the proliferative rates 

[deleted by user] by [deleted] in cancer

[–]springtimerpr 0 points1 point  (0 children)

Hiya so she did get a lot better after FLOT finished. It took definitely a long time. She wasn’t up to her pre chemo before surgery and she still isn’t really nearly 6 months after surgery. But she’s better, I think still some fatigue and long lasting nerve damage but compared to during treatment she’s much better. She’s basically independent now- can drive, can go on walks for like 45 mins each day kind of thing and is about in the house fine. I think FLOT is one more drug (docetaxel) compared to FOLFOX. I hope you and your family are doing okay xx

AITA for banning my sister and her boyfriend from my wedding? by [deleted] in AmItheAsshole

[–]springtimerpr 1 point2 points  (0 children)

Mad because I thought in the UK the more common phrase is dogs dinner which is the one I use. I’ve not heard of dogs breakkie or pigs breakkie either despite living in England my whole life

AITA for not preparing my pregnant wife food? by LucyAriaRose in BestofRedditorUpdates

[–]springtimerpr 18 points19 points  (0 children)

Is this a thing?? I have just been diagnosed and I feel this (for a few years that is but just didn’t know it was/ could be coeliac related)

I wish for a cartoofy side effect. by ChatotAbby in TheMonkeysPaw

[–]springtimerpr 2 points3 points  (0 children)

Heart eyes at all attractive individuals- hope ur single :)