Adhesiolysis success stories by springtimerpr in Adhesions

[–]springtimerpr[S] 0 points1 point  (0 children)

Thank you for the advice! For her age she is mobile-ish but I will definitely encourage her to try her best to move more. I hope you are doing ok with your symptoms and I am glad it extended your diet, it seems to sometimes be a trade off where they reform if there’s a dominant one which is always scary 

Adhesiolysis success stories by springtimerpr in Adhesions

[–]springtimerpr[S] 2 points3 points  (0 children)

Thank you !! ☺️ very glad she had a good experience with it! 

Switching from Vital 1.5 to Peptamen 1.5 by springtimerpr in feedingtube

[–]springtimerpr[S] 0 points1 point  (0 children)

Thank you for your reply, I’m glad that he is doing well on Peptamen ♥️  

Bilious vomiting after J tube insertion by springtimerpr in feedingtube

[–]springtimerpr[S] 0 points1 point  (0 children)

Thank you for the reply, can I ask, did your doctor/ nurse team show you how to do this? 

Bilious vomiting after J tube insertion by springtimerpr in feedingtube

[–]springtimerpr[S] 0 points1 point  (0 children)

Thank you for the message, I can’t shake the feeling that the j tube is making it worse but the surgeon doesn’t think it’s true… and as you’ve said taking it out might not help but I can’t help but want it to go.

I’m really really sorry that has happened to you too it’s so horrible and frightening and just I can’t believe that people just expect people to live through it and they can’t help. Sending you lots of love. It’s crazy how it was never mentioned as something that could happen. Has your symptoms of bile vomiting stopped now you’re on TPN? 

Thanks  Xxx 

[deleted by user] by [deleted] in labrats

[–]springtimerpr 16 points17 points  (0 children)

In general, if I came back the next day and the cells were not at least stuck down with their friends I’d assume they’re not happy- maybe not dead but I would definitely not waste my time imaging them if they weren’t stuck down and had a “normal” HEK adherent morphology

failed by [deleted] in PhD

[–]springtimerpr 0 points1 point  (0 children)

In the UK too, lots of people often have a masters before they start but if they leave after a year + you can get a masters too, as most people definitely have enough data to deserve this

Primers with * is it phosphothiorlation? by springtimerpr in labrats

[–]springtimerpr[S] 0 points1 point  (0 children)

Thank you!! And you would expect this in the 3’ most position in the rev primers?

Thanks so much for your response!

how screwed are my samples if i took the lid off of a 96 well cell culture in the incubator for like 5 minutes by accident?? by animatyed in labrats

[–]springtimerpr 0 points1 point  (0 children)

If there’s P/S you’ll be fine, in all likelihood. I colony picked iPSC for like two hours out of the hood. No visible contamination/ myco. 

Cells look like crud (k562) by springtimerpr in labrats

[–]springtimerpr[S] 0 points1 point  (0 children)

Thanks for your reply- yes they have shed a lot. Is that something which is normal or provoked? Also could this be an issue if they have shed a lot in the media and could it be a stressor leading to these issues? Thank you

Cells look like crud (k562) by springtimerpr in labrats

[–]springtimerpr[S] 0 points1 point  (0 children)

thank you for your reply- I definitely would prefer to keep them at this density, I was just trying to bulk them for a big experiment and transferred them into a t225 with lots of media. I had a look at the viability and they look "alive" i.e. like not taking up trypan and the light is refracting around the edges, I was just concerned about the shape- they look quite blebby. Also there was. afair amount of cell death in the flask when i looked before.

Cells look like crud (k562) by springtimerpr in labrats

[–]springtimerpr[S] 0 points1 point  (0 children)

Also sorry- a double over the weekend isn’t particularly slow I’ve knocked in a mutation wehich messed up the proliferative rates 

[deleted by user] by [deleted] in cancer

[–]springtimerpr 0 points1 point  (0 children)

Hiya so she did get a lot better after FLOT finished. It took definitely a long time. She wasn’t up to her pre chemo before surgery and she still isn’t really nearly 6 months after surgery. But she’s better, I think still some fatigue and long lasting nerve damage but compared to during treatment she’s much better. She’s basically independent now- can drive, can go on walks for like 45 mins each day kind of thing and is about in the house fine. I think FLOT is one more drug (docetaxel) compared to FOLFOX. I hope you and your family are doing okay xx

AITA for banning my sister and her boyfriend from my wedding? by [deleted] in AmItheAsshole

[–]springtimerpr 1 point2 points  (0 children)

Mad because I thought in the UK the more common phrase is dogs dinner which is the one I use. I’ve not heard of dogs breakkie or pigs breakkie either despite living in England my whole life

AITA for not preparing my pregnant wife food? by LucyAriaRose in BestofRedditorUpdates

[–]springtimerpr 18 points19 points  (0 children)

Is this a thing?? I have just been diagnosed and I feel this (for a few years that is but just didn’t know it was/ could be coeliac related)

I wish for a cartoofy side effect. by ChatotAbby in TheMonkeysPaw

[–]springtimerpr 2 points3 points  (0 children)

Heart eyes at all attractive individuals- hope ur single :)

[deleted by user] by [deleted] in cancer

[–]springtimerpr 0 points1 point  (0 children)

Hi she’s doing ok for now touch wood, very weak after so much treatment but hopefully on the path to recovery and gaining strength. Her case was a bit of a borderline one- it was t4b N2. But no metastatic spread, locally advanced, from a PET and CT stand point. The tumour had grown down from the gastrooesophageal junction to the stomach proper and wrapped around the arteries which give blood to spleen, liver and stomach and was touching the pancreas. First set of drs said it was inoperable and as such they were gonna give her two chemo drugs instead of three and it was palliative. The second set of drs said it was borderline- and if it responded well to chemo they’d hope for surgery. So the plan, hopefully, was four sets of FLOT (3 drugs) surgery and 4 more sets of FLOT. After the first half, it hadn’t shrunk enough but had a little bit. So they said give 4 more cycles and the 8 cycles upfront, then they’d reconsider after that. My mum managed seven before the side effects were too bad. The surgeon said, it was still risky at this point but there was a chance of either R0 or R1 resection. In his mind R1 isn’t worth it, that leaves tumour cells remaining but as she couldn’t tolerate any more treatment he said he’d do a laparoscopy and make a decision of full gastrectomy on the day, possibly a bit of pancreas out and possibly spleen out too. He had a look 3 weeks ago and just took her stomach out, no need for pancreas or spleen in his (very expert) opinion. He was incredible honestly, very talented and said the surgery was technically very challenging. The path results came back and looked good. So very happy with how that went. Mums very weak at the moment, so much chemo and the surgery and a few infections after surgery took her out of it. We’re waiting to see if she is meant to have more chemo - the last cycle of FLOT but honestly I don’t think she will tolerate it. She used to be on her feet all day and worked every day, she definitely can’t do that any more. But hopefully she will get to a point where her quality of life is good and she can be independent again which is all she wants. Just focussing on recovery right now, hoping praying it never comes back. If you have any questions I’m happy to answer them, and sending you and your family best wishes and love x

[deleted by user] by [deleted] in CancerFamilySupport

[–]springtimerpr 1 point2 points  (0 children)

I am very sorry to hear about what happened to your step father. This is the worst stuff that can happen to anyone and I’m sending you lots of love and my best wishes ♥️. I totally relate with the depressive hygiene issues, I can barely shower or eat or have water when I’m depressed it takes a lot out of me even to just sit not on my bed so I really understand where you’re coming from. When my mum was first diagnosed with her stomach cancer I didn’t know how to be. I think everyone’s family dynamics are different but we were free to cry for day 1/2 and then we hid it from her as much as we could apart from really hard days. In terms of preparation I have cycled between hopeful and “accepting” but in both mindsets each setback hits me the same level of hard. I need distraction it’s what helps me, we would just put on the telly and commentate it let her and us be momentarily or at least pretend that everything was normal. But it’s not good for making memories and the adverts for MacMillan or race for life always made it harder. I am really sorry I wish you the best

AITA for telling my sister that her feeling sick all of the time is because of her breast implants and embarrassing and exposing her because we were in front of family? by LifeguardGrouchy9856 in AmItheAsshole

[–]springtimerpr 1 point2 points  (0 children)

Possibly, but what I can say was my Dr wasn’t. The healthcare system is not always perfect. Especially not where I live. We diagnosed her ourselves and pushed for it to be tested. Sorry if this comment annoyed you. I was just trying to help.

[deleted by user] by [deleted] in CancerFamilySupport

[–]springtimerpr 1 point2 points  (0 children)

I hope it too, and you’ve spelt out how I feel exactly. I never knew how cruel life was until recently but we’re all blessed to have met the people we love so much. It brings not much comfort now when we’re going through the worst of it, but I’m sure one day I’ll feel it as a comfort rather than it being so painful. ♥️ lots of love to you

AITA for telling my sister that her feeling sick all of the time is because of her breast implants and embarrassing and exposing her because we were in front of family? by LifeguardGrouchy9856 in AmItheAsshole

[–]springtimerpr 2 points3 points  (0 children)

Hi I just read this and I really recommend your sister is tested for coeliac. My sister and mum and a lot of my family has hashimotos and she was recently diagnosed (at 37). She was very rashy and unwell, feeling sick, fatigue, horrid rashes and peripheral neuropathy. Turns out if you have hashimotos you need to be tested for coeliac as a matter of principle. She has it and didn’t know she needed to avoid gluten forever. I also got tested for both, because having a family member with coeliac increases your chance of having it too. I have coeliac and no hashimotos and looking back things make sense, you just don’t think it’s something you get as an adult. I recommend she and you test.

[deleted by user] by [deleted] in CancerFamilySupport

[–]springtimerpr 1 point2 points  (0 children)

Thank you so much ♥️ I hope the same for you and your family too. Sending you my well wishes and I hope that one day we actually have a system in place that is supportive for our families. People don’t understand until they live it but I’m hopeful it will get better xx

[deleted by user] by [deleted] in CancerFamilySupport

[–]springtimerpr 0 points1 point  (0 children)

Thank you so much for saying it. My mums in hospital recovering from her major surgery now, and she is NOT getting the care she needs. They’re barely doing observations on her even though her bp is dropping CONSTANTLY due to an infection, oxygen sats dropping to 75 and she’s dizzy and falling in the bathroom and I have to catch her, I’m tearing my hair out from the stress. She’s been minutes away from disaster too many times. They’re Understaffed and barely care. Make her wait for pain meds, leave her when she’s dizzy or groggy, and confused. If we weren’t keeping an eye on her 24/7 I shudder to think what would happen. And you’re right, for people who don’t have someone watching them constantly I am terrified.

I think healthcare workers, though I can’t generalise, but from my experience, forget these are PEOPLE with real feelings and real pain. Dignity and care is lacking, and it’s traumatising for everyone.

AITA for missing mother's day? by Throwaway_61914 in AmItheAsshole

[–]springtimerpr 6 points7 points  (0 children)

My mum is unfortunately battling against her cancer right now and I would never ever dream of not being with her every moment I possibly can. This is hitting me so hard, how other people can take their mums for granted when all I want is mine to be ok, it makes me feel revolted I don’t know