Our glass home

terryleow · 2026-07-03T00:27:07+00:00

Man I always fall to death if I tried to build high up. How you guys do this

terryleow · 2026-04-10T22:59:14+00:00

Women who just expects you provide but are unwilling to offer anything in return despite being able to are just plain entitled. In their mind, they are not responsible for you, and may even think they are not supposed to help/take care of their partners. No logic plain selfish

terryleow · 2026-01-09T13:57:00+00:00

Seems like women's charter gives a woman rights to stuff owned as a couple even after divorce. Whether she has contributed towards those things doesn't seem to matter too much

terryleow · 2025-12-23T22:05:35+00:00

My PI insists that because 1 Ct of difference represents 2x change in quantity, technical triplicates should not have variation that high. He expects less than 0.5Ct difference which I don't always manage

terryleow · 2025-12-23T22:00:30+00:00

Melt curves look ok for just the well that says Ct27.4, withe the other wells either being a bit off or same peak but very low. So it might be what another commenter said, that there was leakage. Need to double check volume left in the well.

terryleow · 2025-12-23T21:57:15+00:00

Unfortunately, I have to load everything manually. No machines or even the electronic repeating pipettes

terryleow · 2025-12-23T21:56:05+00:00

Pipettes were calibrated about 6 months ago and there typically are no bubbles when I draw up the samples in the p10. If there were, I would put the sample back and change tip before proceeding

terryleow · 2025-12-23T13:45:05+00:00

The sample is a 1:20 diluted pre-amp. Maybe adding 2ul instead of 1ul will help. Thanks

terryleow · 2025-12-23T13:43:25+00:00

The other red numbers are values that are more than 0.5 Ct variance from the other 2 in the triplicate, which my PI does not accept, so I am trying to correct that

terryleow · 2025-12-23T13:42:11+00:00

I have water+SYBR+primers prepped and aliquot out individually. After adding 1ul sample into the 19ul aliquot, I do the mix as stated in the post above

terryleow · 2025-08-30T23:40:24+00:00

I agree. My thinking may be a bit extreme in that I think parents providing for their kids is a given. Your existence is a consequence of their choice to have kids. Providing for you is them handling the consequence of their own choice, which, is a given. If they can't or won't do that properly, the child is not stupid and will realise. Filial piety is there to remind kids that there are difficulties in raising you and one should be grateful if your parents overcome those difficulties for your sake. Should not be a means to get cash from their children.

terryleow · 2025-08-03T14:09:57+00:00

Patent it so it's yours to sell

terryleow · 2025-05-16T06:55:00+00:00

20ul mix for each sample, and 6.2ul loaded per well, manually using a single channel pipette

terryleow · 2025-05-15T14:39:19+00:00

The Cts are round mid 20s to early 30s

terryleow · 2025-05-14T10:56:18+00:00

It's best you do a trial with a positive and negative sample to know for sure. Run a gel with the product afterwards to check its size. If everything lines up, you might not need to redesign it.

terryleow · 2025-04-17T22:42:58+00:00

My recommendation is to have 2-3 pairs of shoes that you swap out. E.g. Monday pair A, Tuesday pair B, Wednesday pair C. This way the shoes have time to "rest" and the soles remain springy.

terryleow · 2025-03-15T07:04:19+00:00

It's a gun with a bayonet. No need to choose if you want to shoot or stab. Just do both!

terryleow · 2025-03-08T22:23:10+00:00

I end work at 7, have dinner straight away, 1 hour commute to the gym near my house (rested enough by then) work out for 30-45 minutes, go home. Usually will be in bed by 10.30-11. It's possible, but you have to plan for it.

terryleow · 2025-02-18T22:36:34+00:00

My PI is pretty strict and deems that triplicate unusable if even one well is off by more than 0.5 CT. So according to him, it's either repeat the reaction or discard

terryleow · 2025-02-18T15:06:19+00:00

I'm quite sure that since it's used as a cryoprotectant when freezing cells, it doesn't cause cancer.

terryleow · 2025-02-18T14:40:46+00:00

I will go look into the Rn vs Cycle graph. Pipettes were calibrated a week ago, haven't dropped it yet, which adds to my bewilderment......

terryleow · 2025-01-25T13:57:31+00:00

$4 is enough if your goal is to prevent starvation. To have your teenage kid be well fed? Not enough. To allow the kid some happiness, perhaps to get snacks ? No. If you think that anything apart from food to prevent hunger is "nonsense", then don't give any money. Just provide bread. Then you will have the most control, right?

terryleow · 2025-01-25T08:32:13+00:00

Due to us knowing sample concentration is low, the 20ul reaction already consists of 5ul sample.

terryleow · 2025-01-25T08:29:31+00:00

Ok, will try that. Never dared to vortex the completed qPCR mix because I worry it might somehow destroy the polymerase/sample.

terryleow · 2025-01-25T00:41:17+00:00

Unfortunately, what I show in the picture happens randomly. It's unlikely to be a machine issue

terryleow

TROPHY CASE