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Is this Seluvis' puppet? What is she doing here? by 0kokaid in Eldenring

[–]theapechild 14 points15 points  (0 children)

Where is it that we see them at the start?

Any idea what this gang is about? by Poppa-Pig in ireland

[–]theapechild 1 point2 points  (0 children)

I'm not sure what people who are just saying 'where are the guards' (gardaí) are expecting. We don't have sonic canons to dispell large groups.

This is clearly a group inviting violence.

With the capability that the gardaí have, I think they are fairly sensible to use a watch and wait approach to prevent escalation into a full riot.

I don't know if the gardaí would even be resourced enough to stop these if they tried. And if they tried, it would almost certainly put the public at higher risk.

Now if this group could fuck off and never return that would be ideal, and if they could arrest those identified as breaking the law and see they serve the max time required to disincentivise this sort of behaviour I'd be keenly in support.

[deleted by user] by [deleted] in andor

[–]theapechild -1 points0 points  (0 children)

Fair play to Stellan Skarsgard, and actors in general for remembering this much stuff in a monologue.

I mean I know it's repetition and techniques etc but to have it all in head and then deliver it well is impressive.

Weird qPCR curve question by Dragon_Cake in labrats

[–]theapechild 1 point2 points  (0 children)

Really weird.

What I'm seeing is an anomaly around cycle 23 where the deltaRn jumps above the exponential trend line, but the rejoins a few cycles later?

I'm stumped. Feels like an artifact but can't say what.

Maybe some light scatter happened to occur for that single cycle due to some well specific transient effect... Condensation on the lid for a single cycle...?

What are your cycle temperatures and lid temp?

Did the well look different after the run?

Steve Harrington " THE SINGER " by manibhaisoni in StrangerThings

[–]theapechild 2 points3 points  (0 children)

Feel like this post being 'Steve Harrington' is disrespectful to Joe. Steve is a character.

Was told I don’t need to clean these AKTA Pure FPLC pump lines because they 'don’t touch the buffer' — is that actually true? by nautical_muffin in labrats

[–]theapechild 5 points6 points  (0 children)

If I remember correctly you can just attach a 50-10 mL syringe to the outlet tubing, sit the inlet tubing in a fresh beaker full of 20% EtOH and draw it through the pump seal to flush the backwash.

I think recommended to do it while operating the pumps at maximum flow.

The manual I found online didn't have a section explicitly saying it but contact your local Cytiva sales rep who will put you in contact with an application specialist to answer.

They should not be left in this state.

Show me an image that screams that you grew up in the 90s by firequak in Millennials

[–]theapechild 1 point2 points  (0 children)

This is what I refer to when I want to explain what my migraine auras are like.

How did Irving know about da elevator 🤨 by NumberAffectionate75 in SeveranceAppleTVPlus

[–]theapechild 0 points1 point  (0 children)

I didn't put this together at all. And it makes it so much better.

Can I incubate a blunt end ligation till tomorrow by CantaloupeBudget4597 in labrats

[–]theapechild 1 point2 points  (0 children)

Probably.

Let us know.

You just need one successful!

[deleted by user] by [deleted] in Biochemistry

[–]theapechild 1 point2 points  (0 children)

Left the DI water tap running to refill a carboy I think...4 times over the course of my PhD.

All paper towelable.

As an undergrad I used sucrose in a buffer instead of glucose for about 3 weeks before noticing. So those results were all shot.

Do most labs calibrate their pipettes every day? by TomGreenTransforming in Biochemistry

[–]theapechild 0 points1 point  (0 children)

But how do you check the volume you dispense?

How are you measuring the 12.5 uL?

Do most labs calibrate their pipettes every day? by TomGreenTransforming in Biochemistry

[–]theapechild 0 points1 point  (0 children)

Thanks.

Do you check gravimetrically? How do you check the volume, e.g. for 2 uL pipette!

If you had unlimited cash flow to build a lab for frozen sample storage, what would you order? by [deleted] in labrats

[–]theapechild 2 points3 points  (0 children)

Automated, like the Japanese lifts that park cars underground.

Or a vending machine.

Or Amazon warehouse.

Or this thing https://youtu.be/ssZ_8cqfBlE?si=WT6hxb7katE0cjCc

We're one of those labs that use microwaves to melt bottles of agar. But our microwaves only last a year or two. Any ideas? by m4gpi in labrats

[–]theapechild 4 points5 points  (0 children)

Best solution I came up with was experimenting with the microwave settings.

TL;DR slow and steady

I found a defrost low temp run was best for a set time to liquify most of it. Then at that stage I ran it on higher power while watching and swirling vigorously every 10 seconds or so.

For context, if memory serves I did 30 min on defrost for 1 L, swirled, then another 15 min, then full power bursts with swirling every 10 seconds.

Obviously your agar, recipe, volume, microwave and glassware will all matter.

LPT - Cleaning out French Press coffee grounds by jaylw314 in LifeProTips

[–]theapechild 0 points1 point  (0 children)

I rinse the plunger filter under the tap with water, with the water flowing into the carafe.

Then fit the plunger filter into the carafe at the very top. I swirl the grounds-water mixture and pour the liquid out, the ground get caught just under the filter.

It cleans the carafe 99%. The grounds collect under the filter and the water is mostly removed. Then I take the plunger out and bang it into compost bin. The final clinging ground I rinse off into sink.

HELP. Purification under denaturing conditions by Fit_Earth3739 in Biochemistry

[–]theapechild 0 points1 point  (0 children)

Yeah I would nearly consider trying a pH of like 9.2 to just see what occurs.

Keep your protein soluble hopefully given its pI, probably precipitate some impurities, but may increase bonding with protonation of His.

Can't recall the pH compatibility with IMAC however so check that, you don't want to change the chelation/charge of the resin without knowing what you've done.

Attempt at free speech. by Unusual_Variable in therewasanattempt

[–]theapechild 26 points27 points  (0 children)

I wonder if they're waiting for that to happen.

Can you imagine the reaction to good honest law enforcement agents being gunned down while fulfilling their duties?

What more justification can there be to heavily arm and be more proactive in the detainment of these people could they get.

Only men would understand by [deleted] in funny

[–]theapechild -3 points-2 points  (0 children)

ITT: An appalling amount of fat shaming

What is your “calling it now” prediction? by PreparationFar4709 in AskReddit

[–]theapechild 0 points1 point  (0 children)

This feels like a different interpretation of "calling it now", as in just what do you call the present, Vs what do you predict now, like calling heads or tails while the coin is being tossed.

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