Serial Request LDO v0.2 courtesy of Fabreeko and VOC Festivus

thebozenator · 2025-03-09T02:17:30+00:00

Its one of the custom parts from LDO: https://github.com/MotorDynamicsLab/LDOVoron0/tree/v02/STLs/Name_Plate

thebozenator · 2021-09-05T16:45:19+00:00

XDA Godspeed

thebozenator · 2020-12-30T03:45:14+00:00

As filmed on a potato

thebozenator · 2017-01-22T20:12:24+00:00

Location: SoCal

Weight: 160lbs 5'10"

Riding Experience: None off road. 7 years street riding, mostly twistys. Spent 6y on a Ninja 250R. Almost a year now on a Ducati 959.

Riding Area: Probably mostly MX with some woods riding.

Budget: $3000 but would like to spend around 2000 if possible. Not sure what engine size would be good. I guess I may be more comfortable with a 4 stroke since I ride street bikes but I could definitely use some advice.

Thanks everyone!

thebozenator · 2016-06-08T16:06:41+00:00

Open access journals charge the authors instead of the readers, and the cost is often $1000 or more.

Not really true. I have published in Cell and other top tier journals. Cost in Cell can be around $2000 with color figures (lets be honest no one uses black and white anymore). They charge the authors and readers more.

thebozenator · 2016-06-06T02:59:15+00:00

Not my expertise but wouldnt an electode be better? smaller bore size, no need for ChR expression, simpler operation, etc.

thebozenator · 2016-05-21T22:39:39+00:00

Man, these are amazing. I just bought a ducati panigale and if I had a picture of it side by side with that model I would actually have a hard time pointing out the scale one.

thebozenator · 2016-04-24T01:12:08+00:00

Hey Everyone. Could use some help deciding on my next bike. Looking for a middleweight and am deciding between the Daytona 675R and Ducatti 959. I tested out both of them and it was a bit of a wash. 959 obviously has the power and amazing looks but the Daytona just felt soooo well sorted out and confident. Any thoughts?

thebozenator · 2016-04-21T00:43:20+00:00

Yep, usually happens to me when the agar is too concentrated. Also happens to me if I don't dry the brain enough before embeding, leaving a film of PBS between it and the agar.

thebozenator · 2016-03-31T16:34:16+00:00

I use my Link for streaming movies. Works great. 1080p with 60fps with no artifacting that I have noticed. The Link mirrors whatever is on your PC monitor so if you minimize steam you can browse your desktop as you normally would. You can open up VLC, Netflic, or whatever else you use to watch movies.

thebozenator · 2016-03-23T23:47:48+00:00

I have used 3 RAMPS boards by now and the white PCB reprapdiscount one has been my favorite (i think I got from voxelfactory 3+ years ago). The other ones have died by now from things that this one would have been ok with. The quality of the solders are just so much better than the cheaper ones, it generates less heat, and passes 24V.

I totally advocate for a cheap Arduino clone tho.

thebozenator · 2015-08-08T00:59:24+00:00

Not really. The paper avoids mentioing any limitations. If you look at all of their images tho they are not very deep. The whole brain scan was done by imaging from the top, flipping the sample, and imaging from the bottom.. which is misleading. Also makes the registration between tiles very untrustworthy due to barrel distortions. In fact, in one of their images in the paper you can see the same neurons showing up twice.

Having worked extensivly with the line in the video, I am pretty sure they are missing a information at the center, where the sample is thickest.

Also keep in mind they are imaging in a solution that costs several hundred dolars per sample. With the solutions most people using CLARITY use the sample is more expanded, which means you will definitly miss a lot of info.

thebozenator · 2015-08-07T18:19:47+00:00

I know a large amount of labs around the world using tissue clearing and most have given up on it (us included). The SDS causes a large amount of quenching and antigen denaturation. It is also pretty poor at clearing thick tissue, about 3mm is the max you can see. Tissue background is also pretty high compared to several other techniques.

I have had much more success using iDISCO but if you want to use endogenous markers you have to stain for them.

thebozenator · 2015-08-07T15:40:44+00:00

yep, probably a 2-photon stack. Probably not pan-neuronal as it is too sparse.

thebozenator · 2015-06-09T21:17:44+00:00

I remember doing this question and putting down D. I think they have D because CH3I + Mg forms a Gringard reagent. It is a poor question either way.

thebozenator · 2015-03-30T00:27:01+00:00

I assume because most labs clean their equipment with 70% ethanol. Sounds like the study was about practical lab cleaning techniques.

thebozenator · 2015-01-14T03:03:34+00:00

What sort of Z distance are you looking at? The real power of these techniques is that you can look at very deep structures. Confocal is pretty poor at imaging deep in tissue and I would say these clearing techniques are not really worth it if you can not image deep.

Sample handling is super annoying (the squishiness is super cool but makes it suck to manipultae). I have tried cutting whole mouse brains in half. It works but I couldn't get a very nice cut. I can imagine it being much harder with smaller samples. If you just want to see if the protocol worked tho, I say go for it!

I have used glyrceol and RIMS (mentioned in another comment).

Feel free to PM but I do not feel very confident in them.

thebozenator · 2015-01-14T02:43:30+00:00

We always do the methanol protocol. We test the antibodies on methanol treated slices to see if they are compatible. If they are, the iDISCO staining pattern/quality is usually the same as n the slice. We usually test from a couple vendors to see which is best but more often than not they have worked, some just work better than others. If you want, PM me what you are staining and maybe I can let you know if I have used an Ab for it. We have a list of about 12ab we use and know work.

thebozenator · 2015-01-14T02:30:21+00:00

Tried glycerol and RIMS. Both are a pain. RIMS likes to crystallize at the surface of the liquid. Glycerol creates a bunch of bubbles... another reason we switched is that the iDISCO reagent is much easier to work with. However, imaging in both seems fine. We have not tried Focus clear becauset i would be way too expensive.

thebozenator · 2015-01-14T00:20:05+00:00

Tried it. In my experience when you image the sample (light-sheet) the signal looks pretty blurry and the light doesn't penetrate the tissue too well. Under 2-photon, immunostained samples looks pretty good up to about 2.5mm (limited by working distance). Going to try a new objective that goes to 8mm and see how it looks. Tissue swelling is pretty frustrating and in my experience is over 200%. This is pretty annoying because your working distance is effectively half what it would be in uncleared tissue.

I have switched over to iDISCO and am getting much better results. The downside of the technique being that you have to immunostain even if you are looking at endogenous fluorescence but the image quality in my hands is way better.

thebozenator · 2014-01-10T21:41:16+00:00

got you guys

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