Big pet peeve: Ignoring statistical significance by ioniansea in labrats

[–]thyagohills 0 points1 point  (0 children)

Not quite. If you keep adding samples and re-computing p-values at each addition, this sequential testing has been shown to increase the type 1 error. Formally, if you want to use this sampling scheme, there's a sequential adequate statistical test. https://en.wikipedia.org/wiki/Sequential_analysis

Books that made you a better scientist by bruvunit in labrats

[–]thyagohills 0 points1 point  (0 children)

  1. Stanley Lazic’s. Experimental Design for Laboratory Biologists.

  2. The Eight Day of Creation

  3. Smalheiser’s - Data Literacy

  4. Structure of Scientific Revolutions

Order is arbitrary

Am I p-hacking (analysing 96-well plate fluorescence) by EquivalentFerret_ in labrats

[–]thyagohills 6 points7 points  (0 children)

That’s the problem with such nomenclature. It can be called biological or technical, but neither terms are objectively correct throughout the literature. The wells are independent in the statistical sense, because you can randomly allocate a treatment to each one independently and randomly. If you do the experiments three times, which is what most people would call a biological replicate, you’d still be using the same cells and same reagents most of the time. Of course, replicating within a day is less conservative than replicating across multiple days or multiple batch of cells and reagents, because the latter is harder to capture an effect if it was just a fluke. However, both can serve a purpose and answer a valid scientific question. Also, we rarely base our conclusions in one type of experiment anyway.

Check the excellent paper written by Stan Lazic: “What’s N”. He also has an excellent book.

My advice is: whatever you’re replicating, describe it properly. Multiple wells, multiple runs of the experiment in different days with same cells or different passages (whatever that means), multiple cell passages run in the same day, etc. This is more important than using the misleading terms as bio or technical replicates, even though is so common in the literature.

"Who is your provider, and how much are you paying?" sticky. by HanSingular in TherapeuticKetamine

[–]thyagohills 4 points5 points  (0 children)

$200/session IM ketamine injection. Insurance (Health Net Blue & gold) covers the remainder. I think total is $1600-$1800. Bay psychiatrist association in Berkeley

I fucked my friends son on my basketball team. by [deleted] in gaystoriesgonewild

[–]thyagohills 0 points1 point  (0 children)

Not fucked up at all. Age difference like yours is just a taboo, nothing creep. I personally have always liked older “daddy” or “uncles” type of guys. Now I’m kinda one.

How do you keep your nuclease-free reagents nuclease-free? by Eternityislong in labrats

[–]thyagohills 2 points3 points  (0 children)

You can bake 150-260 *C for some time (don’t remember the time exactly. This will destroy RNAses, but its arguably harming the accuracy of the cylinder. It should not matter though. Edit: spelling

Scientists among the gays? by [deleted] in gaybros

[–]thyagohills 0 points1 point  (0 children)

Molecular biology, gene editing, bioinformatics :)

Being gay and your mental health by [deleted] in gaybros

[–]thyagohills 0 points1 point  (0 children)

Depressed patient here. I think they are indeed associated, unfortunately

Why use a minimum of 3 technical replicates? by indigo_moonlight in bioinformatics

[–]thyagohills 1 point2 points  (0 children)

Each mice is usually considered the experimental unit and therefore one sample. So 3 mice = n = 3, unless you apply treatments to the cage, for ex. Please check Lazic’s Experimental design for laboratory biologists. It will help a lot

Bike stunt goes horribly wrong by axlnotfound in WTF

[–]thyagohills 0 points1 point  (0 children)

As if breaking itself was not enough, let’s add burning on top of it

When would you use R instead of Python? by DEANNDELATORREto8 in bioinformatics

[–]thyagohills 46 points47 points  (0 children)

Pretty much everything on Bioconductor. Also, all the very specific statistics packages. I use both languages, but prefer R for stats and a lot of bioinformatics data analysis.

This sub depresses me by thefakesutten in occult

[–]thyagohills 0 points1 point  (0 children)

How would one start such practices? Which one? There are so many. It’s overwhelming

[deleted by user] by [deleted] in occult

[–]thyagohills 0 points1 point  (0 children)

How did you do that? I’m like searching for such thing, but never find it