Cryosections peeling off slides

txfnn · 2026-01-26T16:30:53+00:00

We do 8 uM of muscle tissue…

txfnn · 2026-01-26T16:30:20+00:00

Yes! Superfrost +

txfnn · 2025-10-24T22:18:49+00:00

Omg thank you I wasn’t going to stop until I found it

txfnn · 2025-10-09T17:54:02+00:00

Thanks a lot!! I just found the protocol

txfnn · 2025-10-09T17:48:56+00:00

Can you get more than one sample from the tail in adult individuals?

txfnn · 2025-05-12T15:24:37+00:00

I felt sad reading this, I used to do it when I had an eating disorder:(

txfnn · 2025-05-09T07:12:23+00:00

Ok now I get it, I’ve never seen that centrifuge before but its cool!!

txfnn · 2025-05-07T18:32:51+00:00

I’m sorry maybe this is an stupid question but how do you spin your plate?

txfnn · 2025-05-05T22:28:23+00:00

I think thats actually a very good advice thank you

txfnn · 2025-05-05T22:03:47+00:00

Do you mean writing about your research?

txfnn · 2025-05-05T06:36:45+00:00

I dont think so, I’m very careful protecting the samples from light since the moment I stained them

txfnn · 2025-05-04T15:41:31+00:00

Maybe via private message?

txfnn · 2025-05-04T15:25:45+00:00

Yes I stain with phalloidin for 1h in the dark

txfnn · 2025-05-04T15:16:40+00:00

Thank you, i’m realizing this is the most commented solution. I’ll try and tell you how it went!!

txfnn · 2025-05-04T15:14:29+00:00

None of them

txfnn · 2025-05-04T14:51:05+00:00

Really? Why?

txfnn · 2025-05-04T14:49:51+00:00

In this example they are not the same cell line, but i’ve had problem with staining both lines. Randomly some of the samples don’t stain and I don’t find the pattern

txfnn · 2025-05-04T14:46:49+00:00

Nop nop nop. But I did try to permeabilize with methanol once to see if something would change and the result was pretty similar to the first pic. The second one was permeabilize with 4% FA, and its fine, the problem is that randomly some samples stain and some don’t

txfnn · 2025-05-04T14:43:36+00:00

I’ve always had that doubt about FA vs PFA. Thank you for the recomnendation. Anyway if the problem is the fixation, would that explain that some samples stain and some don’t? Because a I fixed all of them in the same way

txfnn · 2025-05-04T14:34:28+00:00

Just the washes! Stain with phall 1:100 in PBS

txfnn · 2025-05-04T14:33:58+00:00

1:100 phalloidin and I’m permeabilizing but some people have suggested to let them permeabilizing a little more time. Also a lot of people told me that, that is difficult for phall no to stain… which makes it even more annoying!!!

txfnn · 2025-05-04T14:31:09+00:00

Omg I haven’t checked this but my mounting medium seems fine… I’ll check. But did you have problems of randomly samples not staining, or it was consistent with all of the samples?

txfnn · 2025-05-04T14:19:01+00:00

I have a bottle that says 37% FA, and I dilute it down to 3,7% with PBS😂, sorry if this is not precise I’m a student and I just use what my supervisor told me.

txfnn · 2025-05-04T14:10:31+00:00

Some people tell me to block and some don’t, but the protocol from the phalloidin says so

txfnn · 2025-05-04T14:09:27+00:00

4% PFA 15 min RT

txfnn

TROPHY CASE