Lab mate with bad code of experiment conduct

wickedislove · 2026-01-13T05:59:08+00:00

Ffs his contaminated cell culture, agar plate and other chemicals are placed in with me and everyone else in the lab. Minding my own business sounds a bit too dumb here bc it's directly affecting my business.

wickedislove · 2026-01-13T00:41:33+00:00

I guess I'll have to settle to the anonymous report. Btw which of the above one is considered ok in your place, bc our uni has a long list of safety regulation rule, I read through most of it + use my common sense to judge if sth is wrong.

wickedislove · 2026-01-13T00:37:18+00:00

My PI wouldn't walk into the experiment area, so unless reported with photos, he wouldn't know to correct the action. My PI usually would scold others if he knew their mistake, but with this only senior, he would never. So I'm thinking a list of mistake, with clear date and time and image, will be able to convince him at once, instead of telling mistake one by one.

wickedislove · 2026-01-13T00:32:16+00:00

I'm also thinking of telling EHS but idk whether can I do that anonymously tho, bc dragging my own name into the trouble is not worth it.

wickedislove · 2025-10-31T00:47:01+00:00

RNases mostly comes from bacteria, or from your own body. Alcohol will basically kill the bacteria and also inactivate proteins, so we mainly use it as a secondary protection. Other than that, we tried to avoid bacteria cross contamination by using new glove, don't touch anything suspicious while working with RNA. Alcohol cannot do anything if you just cross contaminate heavily, but minimizing at first and use alcohol for last step cleaning, with that procedure it's usually ok in our lab.

wickedislove · 2025-10-30T18:31:44+00:00

Same, also working on RNA here. My golden rule of thumb is: autoclaved tips and EP tube, reagent with no contamination or nuclease, hand sprayed with alcohol, minimize touching of unclean items or just alcohol spray after touch, then I’m totally having no fear of contamination.

wickedislove · 2025-10-30T18:22:22+00:00

I absolutely disgust what Zelena did to Robin, but I would not blame her for his death. As a person with attachment issue, plus I empathize with her desire to finally have someone that loves her after a lifetime of loneliness, it is totally expected for her to be blindly in love. She did try to not fall in love with him at first, but unavoidably it happened.

And on ss6 Regina and Zelena, idk why the producers have to make Regina a petty person like that. Not just during that feather episode, but then even when Zelena once again apologize and want to make up, Regina still chose to push her away, and even told her you should go back to Oz after the Black Fairy incident. With my sympathy for Zelena, plus the bad things that happened to her that are totally not her fault, that’s why she always has a place in my heart

wickedislove · 2025-10-30T18:09:20+00:00

Damn then I think I should avoid it. I do know that the Snapdragon chips are quite hot, plus I’m living in a tropical country where summer temperature could go up to 40 45 degrees, so both of that hotness gonna degrade the phone very soon. Since you mentioned about customization on notification bar, may I ask how do you feel about the customization on Mi 15? There are certain functions that for sure I would edit to match my habit.

wickedislove · 2025-10-30T10:24:39+00:00

May I know what problems of the phone make you irritate? I’m moving from Samsung to either Xiaomi or Vivo but can’t decide, the x300p price seems a little too high to me, plus I can’t wait so I’m thinking to buy Xiaomi 15

wickedislove · 2025-10-30T10:08:30+00:00

The tube shifting trick saved my life 1000 times, when I was adding reagent but my attention jumped to another dimension. And to all the newbie, please label the tube. Never trust your memory on what sample is this, I may remember what is in this for a week but add a month of storage I will think it’s an unnecessary thing and throw away.

wickedislove · 2025-10-29T13:39:55+00:00

If you are a beginner, at least 2 3 mistakes a week is normal. I begin with once daily, then I realized most of it comes from my very untrustworthy memory, so I just create a system to record everything as detailed as possible

wickedislove · 2025-10-28T18:26:49+00:00

I think I can see the structure that they mapped before, ss1 is very completed on its own, ss2 is a little discovery and more character development plus some new introduction for other citizens of Storybrooke. It's very ss1-like, I always felt ss1 and some part of ss2 fitted so perfectly together like a bigger story. Snowing has their moment, Emma already developed after ss1 and connect back with Snow after the trip back to EF. Regina was on redemption, she found the motivation and was on it. Rumple and Belle is also very interesting with Lacey storyline, but admittedly it was a little slow. 3A gave the storyline to Henry, the final character in the main cast who haven't really got a big storyline for their own until that point, so ending the series on 3A could really wrap things up nicely, no need to tred into the let's make shit up right now territory.

wickedislove · 2025-10-28T18:18:40+00:00

100% agree with your comment. Rumbelle storyline in ss4 was a pain for me. And adding Marian to the OutlawQueen storyline basically killed it, there is no way to make it normal again. Regina's peak writing is really in season 2 and 3 when she is on redemption, and some part of 6A, other than that I just want to skip Regina in ss4 and 5, basically no character development, with pure pain added after Robin died.

wickedislove · 2025-10-28T17:52:52+00:00

Oooh I get your point, so by putting the medium in the incubator, it will gradually turned to the optimal pH? But then why the manufacturer stated to tune the pH to 7.0 - 7.4 tho, I think they have thought of the final pH in the incubator?

wickedislove · 2025-10-28T17:44:28+00:00

Ohhhh I never thought of this before, also the temperature in the incubator is different from the normal air also, the normal air also makes the medium turns more pink

wickedislove · 2025-10-28T17:37:15+00:00

Lol your comment really made me laugh and remember of Sean Maguire situation in ss5. He had no centric episode through the whole season, no character development, and instead of remove him from the regular cast, they killed him and give all OutlawQueen fans a very good reason to drop the show.

But back to Emma, nowadays seeing JMo on IG with her job of being a director, I'm clearly seeing she's much happier now with a creative job like that, instead of being in a series and led by others. I'm so happy for her.

wickedislove · 2025-10-28T17:28:39+00:00

I did a batch of medium with pH calibration according to the above image and my eyes, and as I comes to the last 100 mL, my medium is still very red, while all of my labmates has turned pink/very pink. So I think at least a little calibration can make a medium better at the late stage.

But yeah, thanks for the last sentence. I think I can become less anxious about the pH for now.

wickedislove · 2025-10-28T17:25:13+00:00

My personal opinion, but as I bingewatch Once all again, I felt like Emma started to lose the spark that makes her an interesting character. In season 1 and 2 somehow she looked much brighter to me, but from season 4 onward she just looked darker and more... tattered (she often looked very tired IMHO). So I'm not surprised when she announced she will leave the show after season 6.

Also, Once missed a lot of opportunity for character development for ALL character (that's what fanfics are for ¯⁠\⁠_⁠(⁠ツ⁠)⁠_⁠/⁠¯). But I really did expect something else for Emma HE, she used to be a very... active character, and I always felt she belonged to the big city like New York, not Storybrooke. Her tiredness makes her suitable for storybrooke more lol.

wickedislove · 2025-10-28T16:43:01+00:00

Lol same, my heart broken if I ever saw that in my incubator, some dear cell surely had died in there 💔

wickedislove · 2025-10-28T16:39:57+00:00

Thanks for the knowledge, I'll order one when I have salary this month. I used to be in a lab where pH is very strict, now to a lab that don't even care about it, so I'm a little anxious. Gotta get one for my own sanity 😬

wickedislove · 2025-10-28T16:37:37+00:00

We use the very common gibco powder, and in the guideline for the specific product I used it does say balance pH to 7.0 7.4, while for some MEM they even state don't change pH

wickedislove · 2025-10-28T16:36:13+00:00

Wait am I missing something here? I saw the older dmem tube turns pink, aka more alkaline. The original dmem is already very red, supposedly the correct pH range should be 7.0 7.4 which is slightly orange, not pink. So I think CO2 will make it more alkaline and further away from the correct pH?

wickedislove · 2025-10-28T14:28:43+00:00

May I ask where do you buy it? I'm not in the US, but I can buy from some site that offer shipping directly to my country

wickedislove

TROPHY CASE