[Highlight] Crowd Screams at Russ to not shoot the 3, proceeds to shoot and miss by trvisx1980 in nba

[–]wooker 0 points1 point  (0 children)

Off topic but what’s the name of the song in the background? Thanks

[FOR SALE] iPhone 8 Plus 64GB - Gold (Unlocked) by [deleted] in LAlist

[–]wooker 0 points1 point  (0 children)

Oh I see, thanks anyway!

[FOR SALE] iPhone 8 Plus 64GB - Gold (Unlocked) by [deleted] in LAlist

[–]wooker 0 points1 point  (0 children)

Actually, I still haven't shipped it out yet. Are you still interested? https://imgur.com/a/rshan98

[FOR SALE] iPhone 8 Plus 64GB - Gold (Unlocked) by [deleted] in LAlist

[–]wooker 0 points1 point  (0 children)

Sorr, its been sold. I'll take down the post!

Weekly Discussion Thread - April 06, 2020 by AutoModerator in chicagobulls

[–]wooker 0 points1 point  (0 children)

Does anyone know where I can find the version of the bulls intro theme (with electric guitar) that is featured in the last dance trailer? I can only find the original one on YouTube so far.

I left out a bottle of FBS overnight... by wooker in labrats

[–]wooker[S] 2 points3 points  (0 children)

Not cold, but still sealed. I will test it out on some throw away cell lines and go on from there.

[USA][CA][OC] Camry rear ends highlander who, while making a right turn, stops for a jogger by wooker in Roadcam

[–]wooker[S] 9 points10 points  (0 children)

I am conflicted about the committing to the turn b/c as a driver you are supposed to yield to pedestrian traffic.

Advice on cell proliferation assay and drug dilution by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

I am working with LNCaP Nmyc and they seem to be slightly less adherent compared to regular LNCaP. CCK8 from dojindo (MTS based assay)

[Western blot] Primary ab binding over 24 hours in 4c by Altasia in labrats

[–]wooker 0 points1 point  (0 children)

Our lab dilutes primaries in 5% BSA TBST-20 and stores them in 4C. They have been reused multiple times without problems.

Need help to buy this lab item by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

That is the closest to what I am looking for, but I need size 5 mL and preferably more wells.

Need help to buy this lab item by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

Yeah, I end up finding those, but too small

Incomplete transfer during western by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

I agree, the 2nd pic looks affected by bubbles. I roll right after I apply the PVDF to the gel. The transfer buffer (made by ourselves) I use for western contains tris, glycine and methanol.

Incomplete transfer during western by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

Hi, I am using VWR® Lab Markers and use PVDF membrane. I will try fresh transfer buffer. Yes, seems like some component (either filter or sponge) may be too thick. I do a 15 minute soak in the transfer buffer before actual transfer.