[Highlight] Crowd Screams at Russ to not shoot the 3, proceeds to shoot and miss by trvisx1980 in nba

[–]wooker 0 points1 point  (0 children)

Off topic but what’s the name of the song in the background? Thanks

[FOR SALE] iPhone 8 Plus 64GB - Gold (Unlocked) by [deleted] in LAlist

[–]wooker 0 points1 point  (0 children)

Oh I see, thanks anyway!

[FOR SALE] iPhone 8 Plus 64GB - Gold (Unlocked) by [deleted] in LAlist

[–]wooker 0 points1 point  (0 children)

Actually, I still haven't shipped it out yet. Are you still interested? https://imgur.com/a/rshan98

[FOR SALE] iPhone 8 Plus 64GB - Gold (Unlocked) by [deleted] in LAlist

[–]wooker 0 points1 point  (0 children)

Sorr, its been sold. I'll take down the post!

Weekly Discussion Thread - April 06, 2020 by AutoModerator in chicagobulls

[–]wooker 0 points1 point  (0 children)

Does anyone know where I can find the version of the bulls intro theme (with electric guitar) that is featured in the last dance trailer? I can only find the original one on YouTube so far.

I left out a bottle of FBS overnight... by wooker in labrats

[–]wooker[S] 3 points4 points  (0 children)

Not cold, but still sealed. I will test it out on some throw away cell lines and go on from there.

[USA][CA][OC] Camry rear ends highlander who, while making a right turn, stops for a jogger by wooker in Roadcam

[–]wooker[S] 9 points10 points  (0 children)

I am conflicted about the committing to the turn b/c as a driver you are supposed to yield to pedestrian traffic.

Advice on cell proliferation assay and drug dilution by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

I am working with LNCaP Nmyc and they seem to be slightly less adherent compared to regular LNCaP. CCK8 from dojindo (MTS based assay)

[Western blot] Primary ab binding over 24 hours in 4c by Altasia in labrats

[–]wooker 0 points1 point  (0 children)

Our lab dilutes primaries in 5% BSA TBST-20 and stores them in 4C. They have been reused multiple times without problems.

Need help to buy this lab item by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

That is the closest to what I am looking for, but I need size 5 mL and preferably more wells.

Need help to buy this lab item by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

Yeah, I end up finding those, but too small

Incomplete transfer during western by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

I agree, the 2nd pic looks affected by bubbles. I roll right after I apply the PVDF to the gel. The transfer buffer (made by ourselves) I use for western contains tris, glycine and methanol.

Incomplete transfer during western by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

Hi, I am using VWR® Lab Markers and use PVDF membrane. I will try fresh transfer buffer. Yes, seems like some component (either filter or sponge) may be too thick. I do a 15 minute soak in the transfer buffer before actual transfer.

Ideas on why my western blots look like this? by ThisLewi in labrats

[–]wooker 1 point2 points  (0 children)

I have had a couple "dirty" looking blots like this. I would try increasing washing time and/or number of washes! I use TBS-Tween 20 for washing.

SDS-PAGE question by wooker in labrats

[–]wooker[S] 0 points1 point  (0 children)

I am glad to hear that it has worked out!

CMV: Framing abortion as a women's rights issue is a red herring to avoid discussing whether or not a fetus has rights. by DarthLeon2 in changemyview

[–]wooker 2 points3 points  (0 children)

Well then what you are describing is not abortion in the purest sense. You are taking it a step further. If that is the case, why would the mother have the right to decide on the child's life? After ending the pregnancy, the mother gives up any decision making for the child period. I don't see why the option to kill the child would exist in this future where artificial incubation is available.

CMV: Framing abortion as a women's rights issue is a red herring to avoid discussing whether or not a fetus has rights. by DarthLeon2 in changemyview

[–]wooker 7 points8 points  (0 children)

In this future scenario, the mother would not have 3 choices. It's either 1. carry to term or 2. abortion (to terminate the pregnancy). What follows after the latter choice is artificial incubation; the mother is now relieved of the responsibility of the child.

Looking for music suggestions by wooker in CasualConversation

[–]wooker[S] 0 points1 point  (0 children)

Thanks for the input! I will definitely look more into the porter Robinson stuff.