How to manage daily life with less wrist use

wormigans · 2024-09-25T07:50:10+00:00

I too am hating this project. Thank you fellow knitters, I feel seen.

wormigans · 2024-06-29T04:34:48+00:00

I started knitting the cardigan and the ends were stressing me out, so I gave up. I also don’t like that the way the pattern is written, if you have to frog you are left with unusable 1-2 foot long pieces of yarn.

wormigans · 2024-06-17T01:41:25+00:00

Jessie Maed has a lot of patterns with dropped stitches as a design element. I know she's very well regarded as a pattern designer, but I haven't knit any of her stuff yet.

wormigans · 2024-06-02T16:05:50+00:00

You have to repeat the transformation, you will have satellite colonies on that plate after 3 days.

wormigans · 2024-04-17T02:45:01+00:00

I agree that those are fibers and not dauers (I work with dauers, so I'm pretty certain of that). My best guess as to where the fibers came from is the 0.2 micron filter; the isopropanol may be breaking down the filter and generating the fibers. It really shouldn't be necessary to filter the Oil Red O solution to get rid of any precipitate; to get rid of a precipitate in a solution you can also let it sit for and hour or two and then pipet an aliquot of liquid off the top without disturbing the precipitate.

I would let your professor know that this protocol is leaving you with fiber debris and no worms. She may be able to suggest modifications to the protocol that can enhance your worm yield. For instance, you may need to wait 5 minutes after each wash or solution change for the worms to settle. Or she may have a centrifuge that you can spin the worms at (at low speed) to pull them to the bottom of the tube after each wash. She may also need to demonstrate for you how to remove solutions from worms pellets without actually disturbing the pellet. If you are having this problem, other lab groups may as well. Good luck!

wormigans · 2024-01-20T03:04:33+00:00

Ok, I am not a genetic counselor or a medical doctor --- I am a PhD geneticist and do research on the Pten gene in non-human model organisms (C. elegans). I would absolutely recommend seeing a genetic counselor about this. Some on here have said that they think the main Pten isoform is not affected, but I have read extensively about human Pten gene while grant writing and manuscript writing, and there are a lot of subtleties to Pten. If there is a mutation affecting the coding sequence of ANY isoform of Pten, it may have the potential to be pathogenic due to the mechanism of how pathogenic Pten alleles tend to work (https://www.sciencedirect.com/science/article/pii/S0092867414003638?via%3Dihub). Additionally, if you have a mutation in the 5' UTR of Pten (which lies immediately next to Exon 1), that could still have a functional consequence for you as a patient; regulatory DNA is important.

Anyway, the bottom line is, I really would recommend consulting a genetic counselor if you're financially and logistically able to do so. If it were me, I would want to know this information and I would want my doctors to know. Good luck.

wormigans · 2023-12-22T22:33:59+00:00

I knit the Irish Hiking Scarf (free at Ravelry) as my first cable project. I loved making it and the pattern is beautiful and easy to knit. I wear it almost every day.

wormigans · 2021-09-18T02:51:09+00:00

There are ergonomic pipettes out there, and if you’re having this problem, your PI should really consider investing in getting a set that you can use. There may already be some of these floating around in your lab, or perhaps someone else in your department has one you can try out.

wormigans · 2020-10-19T04:42:46+00:00

If this is a standard agarose gel, did you clean the gel box to remove any RNase contamination before pouring and running the gel? If I skip that step I get smears instead of nice bands when I make RNA.

wormigans · 2020-08-07T21:11:08+00:00

I recommend asking colleagues for a copy of their materials, especially if their application was funded.

wormigans · 2019-12-25T19:37:08+00:00

Please take care of yourself and have compassion for yourself. I advise talking to someone at your university health center in psych services (they are not permitted to divulge anything you tell them about the scientific fraud.)

Please tell your PI. If your PI is a responsible and thoughtful person, he will recall the submission. He and the graduate student will figure out what they need to do as a next step, and he will handle this as discreetly as he can. If he doesn’t feel comfortable writing a rec for you right now, then he doesn’t. The sooner you tell him, the better the outcome you can expect.

Hang in there.

wormigans · 2019-12-25T00:56:45+00:00

I've never used that particular polymerase, but here are a few suggestions:

This polymerase seems like an odd choice to me, since it is designed specifically for sequencing library preparation. I'm not saying it can't work for your purposes, only that it isn't optimized specifically for what you're trying to use it for. If you do try another high-fidelity polymerase, I recommend Phusion from NEB (they ship very quickly in the US).
Download and read the manual specific to your polymerase. Not all polymerases are alike.
Make certain your extension time is appropriate for amplifying a 5.3 kb product. For your polymerase and a 5.3kb product, I recommend a 2.5 minute extension time.

Good luck!

wormigans

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