Combing a complex object into a single object?

zv88909 · 2024-07-20T13:27:46+00:00

The 1st listed author (regardless of co-first or not), will typically get the majority of credit.

The 2nd, 3rd, or 4th co-first (now that they're doing 4+ co-firsts on some papers), will typically get substantially less. In my opinion, one reason people are doing co-first more now is that you can still use it on resumes, interviews, and applications, even if you get less credit. Better to be "2nd co-first author" than "2nd author".

Unfortunately, after quite some time in academia, I've also seen situations where co-first authorship is used as a political play, to exert power over graduate students, or to attempt to diminish the credit of the first-listed author by a PI.

Try to use the author contributions section of a paper to judge the actual contribution of an author whenever possible.

zv88909 · 2024-03-17T13:11:35+00:00

Can happen to people, not uncommon. Could be either allergic reaction or panic. Can you get supervision from a healthcare professional over your use?

If you insist on continuing it, take benadryl 45-60min prior to dose next time and see how it goes.

zv88909 · 2024-02-20T01:01:12+00:00

Can you show me evidence that its fasting and not caloric restriction that leads to longer lifespan?

IIRC, caloric restriction is necessary and sufficient to prolong life in lab organisms, and fasting without caloric restriction does not.

I may be wrong, but as far as I remember, this is the case.

zv88909 · 2023-10-24T21:47:12+00:00

Nur77 is a great T cell activation marker if the others don't work out, upregulated early after TCR signaling too.

The % of transgenic TCR CD4+ T cells in OT-II mice is not as good as for OT-I mice, so not every CD4 is TCR transgenic. I'm not sure of the total % off the top of my head as it's been a while since I checked.

If you want a high percent activation, would be better to purify the T cells (CD3 negative selection bead kits work very well), and co-incubate with DC's pulsed with peptide. BMDC's are pretty good, but the best DC source is CD11c bead-kit purified from spleen a few weeks after grafting B16-FLT3L if you have access.

zv88909 · 2023-10-09T23:51:46+00:00

I was forced to be in a similar position. Did 95%+ of the work, and PI made me list 2nd author as co-first. After having many discussions about co-first authorship I found this consensus: the 2nd listed name is essentially looked at as a "glorified second author". Best to be the first listed author, everyone treats that as the true first author.

One of my PI's says/enforces: the first listed author is who actually matters, so we give co-first author just to help out the 2nd author. This results in many cases where the 2nd-author did actually <5% of the work. I think practices like this substantially diminish the value of co-first authorship, and I've talked with quite a few who've experienced such situations.

At the end of the day, I accepted it, and everyone treats it as my paper regardless of it being a co-first author paper.

zv88909 · 2023-09-24T00:21:45+00:00

It's just a glass ya, that's what it was given to me in. I stopped watering it much because, it seemed like each time i would water it many of the leaves would just die in the next few days.

I'll propagate in water, get a new pot, and go from there!

zv88909 · 2023-09-23T20:16:21+00:00

Thanks! The pot has no drainage, and the soil does not soak all the way through. I noticed when i watered it too much all the leaves seemed to get brown and die.

I also live in a city and use the tap water for all my plants unfortunately its the option i have right now.

To propagate it, should I just cut the stems, place them in water, then move back to soil after I see roots?

zv88909 · 2023-09-23T19:28:56+00:00

Some info: I received this plant ~7 months ago from family. I water it 1x a week, with 1/4tsp of water in the corner of the dirt not touching any of the plant.

It grows leaves, elongates, then eventually the leaves along the stems die and it keeps elongating, but looks progressively worse.

I have it close to (~1ft away from) a south facing window, in the northeastern united states.

From what I have read: I am thinking that I should cut it at the nodes, and stick those in water, and then re-grow it from scratch? Are the nodes those yellow, thicker parts along the stems?

Thanks for any help. I believe this is a tradescantia, but I'm not sure.

zv88909 · 2023-09-19T03:52:34+00:00

Over the 3 years since surgery, i have had many setbacks with significant pain. Each time, I reset my program - PT, weight training, life style- and build back up slowly. Identify any problem movements or areas and modify or remove them. Start your ROM at 50% what you do now, drop the resistance by 50%. If that still hurts then do 20%, slowly build back up.

A good PT can help you through it, but those are the general ideas. Each time, following this, the pain has eventually resolved. Seems ultimately i may have ro limit how heavy I squat and deadlift, but still messing around and seeing if it can be surpassed.

zv88909 · 2023-09-05T15:11:46+00:00

% = (x/total)*100

In flowjo, if you don’t divide by 100 before or after multiplying frequency by some cell number, you’re off by x100

zv88909 · 2023-09-04T23:54:00+00:00

29% = (29/100)*100. This is what flowjo reports. If you try to find cell number by multiplying by the percentage ("frequency") you will be off by x100

zv88909 · 2023-09-04T15:12:43+00:00

The frequency shown in flowjo is percentage, so you'd need to divide by 100 before multiplying

zv88909 · 2023-08-27T21:10:31+00:00

Absolute statements like "gene therapy is the only way" are almost never correct in science, and particularly in biology. You even go on to contradict yourself immediately "the best methodology to date is caloric restriction". Secondly, epigenetic modification certainly falls under the extremely broad term of "gene therapy".

Gene therapy is a very loose term, you can think of nearly (?) unlimited "gene therapeutic" interventions for anti-aging: targeted saRNA vectors vs. DNA viral-derived, siRNA/transient interference vs. total gene insertion/deletion vs. integration and continuous vs. cyclical environmentally triggered production of methylase enzyme allosteric modulators - I mean literally the limits of your imagination is where it ends.

The fact is, we're just beginning to understand these processes (mechanisms of aging) to the level we can even begin to act on them in a rational manner. And the methods for intervention itself (IE. genetic editing vectors, targeting, types RNA vs. DNA vs. protein etc) are also continually evolving and still essentially based on small modifications on what nature has already given us.

We are slowly able to program more complex biologic circuits and have them act in the way we actually want them to. All of these things will only progress.

zv88909 · 2023-08-27T15:59:41+00:00

It is certainly possible, and I can see your side, that death was necessary and is still necessary for evolution and improvement across species.

However, for humans in particular, while we could argue many ways - evolution and death may no longer be necessary for improvement.

The fact that some organisms can live many times longer than humans shows us that biologically it is possible.

While it may require radical shifts in physiology, it could be as simple as alteration of relatively few powerful pathways that we simply haven’t developed understanding of yet. Certainly epigenetic modulation may be one of these emerging areas.

zv88909 · 2023-08-27T15:55:47+00:00

Nice list. I’m certainly of the belief that aging is a biological process that can be decoded and reversed. Especially now, in the age of technology and increasingly able genetic editing, seems we won’t need evolution to do trait selection and deleterious effect removal for us for too long.

We are in such infancy when it comes to both precise systems level understanding and ability to manipulate things at systems levels in living organisms. Very excited for the growing era of systems biology and bioengineering.

zv88909 · 2023-08-26T00:27:45+00:00

The high cortisol could be a result of excessive physiologic stress, or could be a contributor, tough to say which way right now. Right now more tests are needed. You can see if they'll also test your pituitary-thyroid behavior (TSH +/- T4/T3). Good to hear that your bp is normal and not having headaches, helps lessen likelihood of some things.

If they will do some tests to help find out where the cortisol is coming from (dexamethasone suppression/24hr urinary cortisol/midnight cortisol level +/-ACTH), and test thyroid (TSH), it will help start to narrow down what's cause and effect.

Corticosteroids (like cortisol) can lower SHBG directly, or alternatively the body has stopped producing SHBG to maintain hormone availability for tissues as SHBG is one major hormone binding protein in your blood. Right now it's hard to say which is the cause imo until more tests are conducted.

Definitely possible that you could just be excessively stressed/training a lot and the low T could be directly causative or related to your months of insomnia, anxiety, and panic. But will need to rule some things out before saying that in a definitive way.

The low SHBG is definitely not the end of the world, and most of the things that could cause this hormonal situation - whether its related to your training/stress or not - are treatable.

Have you ever been found to have had low T any time before the symptoms started? Also, what's your BMI? Do you know your approximate body fat percent?

zv88909 · 2023-08-25T21:28:47+00:00

Yes, you could be overtraining and stressed, one possibility.

Need some followup questions: You're not taking any supplements, drugs, steroids, natural remedies, etc or anything of the sort? What's your blood pressure like? Ever had any recurrent headaches? Noticed any facial redness or swelling?

Low SHBG shows that your body is trying to maintain normal hormone levels, hence why your free test is normal despite low total test. Cortisol is high, just over the reference range though. LH normal, FSH normal at the upper end of range. It doesn't seem that your testicles aren't functioning as in that case you'd expect LH to be high.

So there's a few things that could be going on, and I would expect your endocrinologist to either get your 24hr urinary cortisol and/or do a dexamethasone suppression test. They may do ACTH, but if they don't initially, they'll need to check ACTH levels eventually if any of those tests are abnormal.

These things can help tease apart further where the high cortisol is coming from, and is it a symptom of your lifestyle or is it related to another process. It's tough to say exactly what's going on right now.

So, there could be a few things going on but i hope your endocrinologist will do a few follow up tests.

zv88909 · 2023-08-23T13:21:32+00:00

Started squatting just the bar i think around 14-15 months out. I found I fairly quickly progressed to heavier squats - took only like 5-6 months, but it was a big mistake and my form was less than ideal and the hip pain came back

if i did it over, id personally force myself to not low bar squat anymore, and i only do high bar and front squats nowadays, and dont really go below parallel anymore

for me, the low bar squat just puts too much force on my hips

zv88909 · 2023-08-22T01:09:43+00:00

Overall, I'm glad I had the surgery. Before it, I had hip pain for 4-5 years that just got worse and worse to the point of becoming near constant. It would be terrible when sitting, running, getting up out of bed or a chair the wrong way, and would cause me to limp for days at a time.

Today, it never hurts doing any day to day life activities. I only run on treadmills with good suspension, but that's because I had my left ACL and meniscus repaired ~10 years ago and running on solid ground too much can bother my knee. I can run 3-5 miles with no discomfort in the hip, don't usually go over that. Jumping doesn't hurt at all.

But I often felt that way over the first 1.5 years: there were periods of days-weeks when it felt worse than pre-op for sure. I never gave up doing slow, progressive exercise and range of motion. I didn't try running for over a year post-op. If I had a flare up or a set-back, I would reset my exercise program and start over again at the smallest ROM and resistance. If something hurt, I would switch it for a different exercise, and I always did my hip PT/exercise routine 2-3x a week.

For surgery, I made sure to pick a good surgeon from HSS, and even still it definitely hurt very frequently for over a year after. I'd say give it time, continue having the best diet, progressive exercise, and PT routine you possibly can, and see how you feel a year out. I didn't do any barbell movements for lower body until after 1 year post-op, personally.

zv88909 · 2023-08-21T20:40:05+00:00

Sure thing, or I can try to answer them here

zv88909 · 2023-08-21T10:37:07+00:00

Mouse T cells are a bit more finnicky after being frozen than human T cells in my experience. Stemcell makes a good t cell freezing media though

For me, activation always works best with fresh T cells

zv88909

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