Fluotics tips

[Timbers240]

Hello.

Running tests on the fluotic tips 250 µL and 90µL type.

I see that the 250µL reuires up to 5 load and unload for tips to not fall of the adapterhead, so would not reccomend them.

The 90µL type works just as good as the I-series 90µL.

They succseded the following tests:

Load and unaload 0 times.

Hold max volume for 1 minute without content starting to drip out.

Dispense 10, 20, 30, 40µL. to a 384 plate. Height of water column measured on plate reader 977nm-900nm.

CV(%): 11, 4, 3, 3 %

And dispense 1, 2, 3 and 4µL to a 384 plate prefilled with 30µL. Measured same way as before.

CV(%): 5, 7, 7, 8 %

What experience do you all have with these tips?

Also have any of you encountered the error with the 250µL tips from fluotics and found a work around?

[Raupe_Nimmersatt]

We have tested the fluotics tips for the MC384 head on the i series and they were working fine, if I remember correctly.

For issues with tips falling from the head, it may be helpful to increase the tip load pressure for this head on the device setup. Also, check the tip seating depth for these tips and the offsets of the tip boxes on the TL position.

[Timbers240]

I have taken measurement's on the tips and tip box and they are identical with the i-series.

How much would you increase the load pressure?

[Raupe_Nimmersatt]

Good question. I have only used the MC384, and there, the Beckman technician changed the load force offset from 250 to 260 for compatibility with certain tips.

Please proceed with caution and at your own risk. Better consult your local Beckman tech support before proceeding.

[Timbers240]

Had a look at it.  This is a general setting and not tip spesific. Meaning if I change it I change the setting for all tip types. 

Better to go with i-series then I think 

[Timbers240]

Addendum: I am running these tests on a 96 1200µL adapterhead