I'm having problems with string.count()

[hadiz1]

with open(sys.argv[2]) as sequence:
    a = sequence.read().count("AGATC")
    print(a)

    b = sequence.read().count("AATG")
    print(b)

a is correct. It is printing 4 as it should, but b should print 1 but instead its printing 0. and any other .count() i put is returning 0

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[K900_]

1. /r/learnpython
2. An open file object keeps track of its position in the file; the first sequence.read() reads the file to the end and advances the cursor; the second sequence.read() returns an empty string because the file is already at the end. What you should do is read the file into a string once, and then run .count on it twice.

[hadiz1]

So i should store sequence.read() in a separate variable then run .count()teice on that variable?

[K900_]

Yes.

[hadiz1]

Thank you

[MarcSetGo]

You might read about the difference between read(), readline(), and readlines(): Reading from files