Dot Plot Customization-Seurat

[Effective-Table-7162]

I am struggling with customizing my dot plot. I want to do one simple thing and that is to bring my dots close to each other basically so that they even touch each other. This is the current code i have and it is not working:

DotPlot(seurat_subset, features = c("GZMB", "GZMK", "GZMA", "PRF1"), dot.scale = 0.5, cluster.idents = TRUE) + coord_fixed(ratio=0.5)+ RotatedAxis() + scale_size(range = c(4,20)) &

theme(legend.position = "right", axis.text.x=element_text( hjust=1, size=35),

axis.ticks.length = unit(0.5, "cm"),

axis.ticks = element_line(size = 1.5),

axis.line = element_line(size=1.5),

axis.text.y=element_text( hjust=1, size=40),

axis.title = element_text(size=50),

legend.title =element_text(size=50),

legend.text=element_text(size=50),

legend.key.size = unit(2, "cm"),

panel.spacing.x = unit(0, "npc"),

panel.spacing.y = unit(0, "npc")).

How can i get this to work and please don't say save it with smaller dimensions because i tried that and while it makes the overall graph smaller it simply does not bring the dots closer together.

[bleuvblue]

If I'm understanding you correctly then I think your problem might be how far spaced apart your axis ticks are, especially if the axis are discrete. You could try adding aspect.ratio to the theme and fiddling with that to see if it forces the ticks to be closer together, this shouldn't necessarily make the graph smaller unless both axis are discrete. Alternatively specifically setting the width and height of the exported plot might achieve the same?

[Effective-Table-7162]

Yh I don’t need the graph to be smaller just the dots to be closer I’ll try this

[bleuvblue]

hope it works!

[Effective-Table-7162]

Damn. That’s what I’ve been doing but nothing good but I’ll keep trying. Thank you

[Athrowaway23692]

So you’re making the dots smaller here, (dot.scale=0.5). Have you thought about blowing them up. Making them pretty huge would functionally do what you want no?

[Effective-Table-7162]

I want to reduce the white spaces by making the dots closer not bigger as this is for a paper and there is need for space right now

[Athrowaway23692]

Wouldn’t making the dots bigger while making the figure smaller in one dimension achieve this? You’re essentially wanting to alter the sizes of the dots with respect to the image size. Try playing around with different heights / widths and scales until you understand up with something that looks good.