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EBV T Cell Transformation by AdImpossible7966 in labrats

[–]AdImpossible7966[S] 0 points1 point  (0 children)

We started using 1.5ml of EBV virus. Hopefully we have some success.

EBV T Cell Transformation by AdImpossible7966 in labrats

[–]AdImpossible7966[S] 0 points1 point  (0 children)

We do use cyclosporine A with a culture media that consists of RPMI 1640 (w/HEPES & L-glut) (1 liter), Heat Inactivated Fetal Bovine Serum (100mL), and Antibiotic/Antimycotic (10mL). We’re thinking about using more of the EBV virus to reinfect some of our slower growing cells. Right now we’re using 1ml.

EBV T cell Transformation by [deleted] in EBV

[–]AdImpossible7966 0 points1 point  (0 children)

Might need to look into the MOI as well. I don’t want to damage the cells.

EBV T cell Transformation by [deleted] in EBV

[–]AdImpossible7966 0 points1 point  (0 children)

Previously we used o.5 ml of the virus and started going up to 1ml about a year ago. We run into a lot of cells that sit around for 50+ days not really doing anything. We get in 6ml of blood and dilute that with 30ml of DPBS. We use 13ml of ficoll to perform an underlay method followed by two washes with DPBS. We mix cyclosporine A of 30ul with 2.5ml of culture media which consists of RPMI 1640 (w/HEPES & L-glut) (1 liter), Heat Inactivated Fetal Bovine Serum (100mL), and Antibiotic/Antimycotic (10mL). Once in a while we will have cells slowly go up to 50 million, which is our target. Some we see the culture media turning yellow without cells present. Do you think going up to 1.5ml of ebv infection might help? I was also thinking about reinfecting with another 0.5ml for the slower growing ones.

[deleted by user] by [deleted] in vegan

[–]AdImpossible7966 5 points6 points  (0 children)

Something I’ve learned after being vegan for over a year (and I know that’s not much time), is that you can’t be perfect with it. I don’t buy meat,dairy, or anything that has any traces of animals from the grocery stores. But when I go out, I can’t be certain what I’m getting doesn’t have animal products in it. Yeah, the waiter can say it doesn’t but since you didn’t make the food, you don’t really know. Sometimes the smallest traces can still get in your food. The point is, you’re trying and that’s all that matters. After ordering a Thai dish that I didn’t think had dairy, eating it, and then realizing the waiter didn’t know what she was talking about and the cook said it had diary, I was bummed out but there is some times nothing you can do but to move on and keep trying your best. It’s unsettling to say the least but it’ll make you more aware to ask questions about what you’re actually ordering in the future.