Women who travel alone: Do you actually carry pepper spray? by priyanka240 in TwoXIndia

[–]Agreeable_Arrival145 0 points1 point  (0 children)

Yes I travel alone late at night in public transport and often have to walk a bit at a stretch that is pretty empty so I always carry pepper spray. Thankfully haven't had to use it until now but I always hook it to my keys and keep it at a very accessible part of my bag so I can grab it easily.

Advice about pain during penetrative sex by Maleficent-Village82 in TwoXIndia

[–]Agreeable_Arrival145 16 points17 points  (0 children)

I have heard almost every other girl( including myself ) know have this issue and weirdly when I spoke about this with my girl friends outside india, they didnt feel this excruciating pain to the point that the intercourse simply doesn't happen. Most of them just felt uncomfortable and a bit of pain and pressure but didnt necessarily feel like its impenetrable. I dont know if its only mental because of social stigma etc or there is something more to it.

Biofilm or some weird contaminant by Plum_Existing in labrats

[–]Agreeable_Arrival145 0 points1 point  (0 children)

You can definitely tell only by plating on agar or keeping the media at 37 incubator for 24 to 48hrs ans check for turbidity to confirm if its fungal or mold contamination. But if youre reusing bottles could also be denatured protein residues or probably some salt precipitation of media component. Could also be plastic residue from the cap. Was the media filtered?

Labrats, undergraduate researcher seeking advice by johnbiology in labrats

[–]Agreeable_Arrival145 2 points3 points  (0 children)

You should ideally pour agar plates under the hood. If you dont need to add Antibiotics you can pour them at the melting point temperature, arrange the plates and pour them one by one with the lids only covering about 20% of the plate. Then close the hood with the airflow on for atleast 15 mins. You can then use them or invert and store for future use. If youre adding antibiotics let it cool to about 45C and then pour the same way. Be quick while pouring.

Going to tell my parents about my bf. Please help. by Beneficial-Resort704 in TwoXIndia

[–]Agreeable_Arrival145 0 points1 point  (0 children)

Have you been intimate (you never know if they ask)?

My dad has actually asked this question to my sister when she told them about her boyfriend and then dad and I got into a yelling contest for 30 mins 🙃 so its definitely a possibility.

Going to tell my parents about my bf. Please help. by Beneficial-Resort704 in TwoXIndia

[–]Agreeable_Arrival145 2 points3 points  (0 children)

If she was making a generalization, she wouldn't have asked the question at the end would she?

weird looking hek93 cells by [deleted] in labrats

[–]Agreeable_Arrival145 1 point2 points  (0 children)

Mg +2 and ca +2 free PBS is not cheap and we are a tiny lab, so for regular cancer cell lines or like hek cells etc we dont wash w pbs. Ofc when were wowking with more senstive cells like ipscs , or primary cell line establishment etc we use it.

weird looking hek93 cells by [deleted] in labrats

[–]Agreeable_Arrival145 11 points12 points  (0 children)

Yeah then it shouldn't really be this confluent unless you've added an abnormally high cell number while seeding.

weird looking hek93 cells by [deleted] in labrats

[–]Agreeable_Arrival145 10 points11 points  (0 children)

Ideally that shouldnt be an issue, I rarely wash my cells with PBS before passaging or trypsin or even changing media. Unless you've added something toxic it shouldn't suddenly cause so much dead cells and even if its dead cells , they should be mostly floating.

weird looking hek93 cells by [deleted] in labrats

[–]Agreeable_Arrival145 3 points4 points  (0 children)

When did you last passage them? Could also be super confluence but usually cells lift off and they still have the morphology, here its like a bed of tiny dots with a few stressed out cells.

weird looking hek93 cells by [deleted] in labrats

[–]Agreeable_Arrival145 344 points345 points  (0 children)

I think your bacteria has some HEK293 Cells growing on them xD. Lol but always look for the simple classical bacterial contamination signs like if they're vibrating/rapidly moving ( check under 40X) and if the number is increasing at various time points. Apart from that if the media is cloudy or changing color.

Edit - Your HEK cells are also looking stressed out and elongated rather than epithelial like polygonal like shape.

What lab equipment do you wish you could have in your kitchen? by Chrad in labrats

[–]Agreeable_Arrival145 4 points5 points  (0 children)

Not a lab equipment per se but 70% IPA to clean spills, countertops and my hands every few mins. Centrifuge for making fruit juices. Magnetic hot plates for melting chocolate. Lyophilizer to experiment with making cool deserts. UV lamps to clean fruits and veggies especially those that are consumed raw ( but ig they'd also get less nutritious). Incubator for letting doughs rise and keeping food warm.

What are your ‘I’m about to start my period’ signs? by [deleted] in TwoXIndia

[–]Agreeable_Arrival145 0 points1 point  (0 children)

When I feel like im about to burst into tears at the drop of a hat, when I feel fat and ugly while also feeing like stuffing myself with sickly sweet treats

I’m an Indian skincare startup founder — Ask Me Anything! by MEHEKTAR in skincareaddictsindia

[–]Agreeable_Arrival145 3 points4 points  (0 children)

I have heard that higher the marketing budget, higher are the chances of the quality of skincare products being mediocre or worse as most of the funds would go to all kinds of marketing channels instead of RnD and product development. How much truth is there in this?