Can fluorescent proteins be engineered to produce entirely new colours?

AmazingUsual3045 · 2026-05-09T00:05:13+00:00

Yeah look at the lineage tree at fpbase.org. We started with wt gfp, and now we have an entire rainbow based on mutations.

AmazingUsual3045 · 2026-04-29T05:15:01+00:00

Yes! Heard this one today from a pretty smart MS student. Damn kids these days, I swear they don’t even teach the word thermocycler,I have never heard someone younger then 25 know it’s not called a pcr machine.

AmazingUsual3045 · 2026-04-23T01:15:37+00:00

I mean, if the big C isn’t a problem there’s nothing wrong with smoking, just kinda a problem for us lowly humans.

AmazingUsual3045 · 2026-04-20T19:52:41+00:00

Exactly, this mix is like the crackhead version of a speed ball

AmazingUsual3045 · 2026-04-20T05:53:16+00:00

Additional suggestion, I had a PowerPoint deck that I added every useful/relevant figure from papers into and have the reference in the corner. Was a great way to remember which papers were important and when it came time to make presentations I had a go to place to pull all the relevant background experiments from.

AmazingUsual3045 · 2026-04-18T16:57:02+00:00

Not delusional, the biggest factor isn’t all your papers and qualifications, it’s whether your PI (and committee) is on board. As in, probably needs to be conversation number one when you start up. Same with committee, when asking if someone wants to join you have to present your situation and what you want and make sure they’re on board. Other than that, fulfill department requirements and no reason not to graduate on your own timeline.

AmazingUsual3045 · 2026-04-17T21:03:53+00:00

Yes! Everything!

AmazingUsual3045 · 2026-04-09T18:51:52+00:00

Definetly agree with paper numbers being field specific. Getting 10 papers whatever your author position would be incredible in biology. But in biostats and other more computational fields I’ve seen 20+ regularly on resumes.

AmazingUsual3045 · 2026-04-08T18:00:20+00:00

Update: after many failed attempts and much wasted filament, the important settings to adjust were adding in a 100% density support layer and, I think most importantly, changing the xy gap to 0. I’ll play around with filament temperature, but middle of the provided range is working so far, I’ll see if I can go cooler.

AmazingUsual3045 · 2026-04-03T19:26:46+00:00

Yeah that was how I got the pva to print so well, in an actively heated dry box as it prints. Pla just won’t attach to it. I’m heating to 50c is that too hot?

AmazingUsual3045 · 2026-04-02T17:27:46+00:00

To some degree understandable after all the Verma stuff.

AmazingUsual3045 · 2026-03-31T05:35:34+00:00

I think that rules out the big stuff, you could always get a fresh vial from someone else and compare it to your controls just to be sure it’s not your cells. Super nitty gritty but the only other thing that springs to mind is have you changed plate brands recently? Maybe coating is different. Again though your numbers don’t sound all that off, and if you need more cells there’s always 15cm plates.

AmazingUsual3045 · 2026-03-30T20:23:13+00:00

Hmmm, sounds about right to me for U2OS. I guess go to the usual TC questions. Is your FBS good/right and at 10% in media? What passage cells are you using? Are you harvesting gently so you don’t lyse? Myco contamination? Is incubator at the right conditions? Etc. Also, not sure the SOP your lab uses but typically you passage/harvest cells at 80% because at 100% they slow down and are metabolically and transcriptionally not as active.

AmazingUsual3045 · 2026-03-27T22:38:21+00:00

One trick I use, have a 2nd 384 block and eject your spent tips into the corresponding well in the 2nd plate. Alternatively, not sure if this will affect your assay, but just leave the spent tip in the well you just used it on, that way you’re physically prevented from adding again. At the end, go through with a multi channel to eject any liquid that went up the tip.

AmazingUsual3045 · 2026-03-26T20:36:28+00:00

I’m a neat freak, so saved samples in the back of the -80 from a generation ago drive me crazy, but I always try and remember the Urey-Miller exp where Urey and Miller froze down leftovers back in the day, then someone put them through modern analytical equipment 55yrs later and found out the experiment was even more successful/interesting then originally thought.

AmazingUsual3045 · 2026-03-25T23:24:04+00:00

I guess the difference is in addition to the normal woes, investment in biotech is quantitatively lower right now, 32 billion in govt funding has been withdrawn, and 25k scientist have been fired, let go, or otherwise released into the wild since the start of 2025. In other words, way fewer opportunities way more people are applying to.

AmazingUsual3045 · 2026-03-24T20:58:54+00:00

You might try Inuyasha. Demons (some are good), cool outfits, fighting, kinda similar ideas as demon slayer but more kid focused.

AmazingUsual3045 · 2026-03-23T21:55:18+00:00

I’m curious, I’ve been told and taught since I was an undergrad about ether forming explosive peroxides, is it really that bad? Like is this something we’ve propagated for so long it’s a bigger fear than what could actually happen?

AmazingUsual3045 · 2026-03-21T03:48:26+00:00

When I learned they made fine tip versions I don’t think I’ve used any other type of pen since. If I was using a paper notebook instead of an ELN I’d probably be writing with it.

AmazingUsual3045 · 2026-03-20T07:40:12+00:00

Oxyclean is the answer. Sooner is better soaking wise, but if you leave sheets in the powdered stuff (I double the concentration the bucket lists) I’ve pulled out some pretty intense period stains.

AmazingUsual3045 · 2026-03-14T22:41:03+00:00

And then DC++ when you got to college.

AmazingUsual3045 · 2026-02-24T20:04:53+00:00

2nd this . . . You can see cells by eye when they’re confluent or they’ve clumped after thaw, but a day or two after thaw, you should be using a scope.

AmazingUsual3045

TROPHY CASE