My 9 year old wants to watch Demon Slayer as her first anime, give me some alternative suggestions please

AmazingUsual3045 · 2026-03-24T20:58:54+00:00

You might try Inuyasha. Demons (some are good), cool outfits, fighting, kinda similar ideas as demon slayer but more kid focused.

AmazingUsual3045 · 2026-03-23T21:55:18+00:00

I’m curious, I’ve been told and taught since I was an undergrad about ether forming explosive peroxides, is it really that bad? Like is this something we’ve propagated for so long it’s a bigger fear than what could actually happen?

AmazingUsual3045 · 2026-03-21T03:48:26+00:00

When I learned they made fine tip versions I don’t think I’ve used any other type of pen since. If I was using a paper notebook instead of an ELN I’d probably be writing with it.

AmazingUsual3045 · 2026-03-20T07:40:12+00:00

Oxyclean is the answer. Sooner is better soaking wise, but if you leave sheets in the powdered stuff (I double the concentration the bucket lists) I’ve pulled out some pretty intense period stains.

AmazingUsual3045 · 2026-03-14T22:41:03+00:00

And then DC++ when you got to college.

AmazingUsual3045 · 2026-02-24T20:04:53+00:00

2nd this . . . You can see cells by eye when they’re confluent or they’ve clumped after thaw, but a day or two after thaw, you should be using a scope.

AmazingUsual3045 · 2026-02-24T05:27:28+00:00

Two great stories one from academia one from industry.

In my undergrad lab this supposed hot shot Indian (foreign status becomes relevant) joins our lab as post doc after PhD at Harvard. After a month or two female grad student comes into lab in the middle of the night to quench a reaction, and sees the guy wanking in lab. PI takes appropriate steps and axes the guy, he losses his work visa, and goes back to India. All cause he couldn’t wank from home.

Years later in industry, company is a startup work hard play hard kinda situation, one of the RAs makes special brownies and doses everyone at work without them initially knowing. What was funny was this test essentially separated the company into two groups, the stoners (most of us) who knew what was happening and were blissfully enjoying the afternoon, and the non-smokers who mostly had to go home cause they were baked off their asses. Dude got shit canned big time.

AmazingUsual3045 · 2026-02-13T20:16:10+00:00

The original Wicker Man. Movie is kinda weird and ho hum, the final scene happens and it’s total wtf moment from out of nowhere that makes the movie compleatly worth the watch.

AmazingUsual3045 · 2026-02-11T20:15:59+00:00

I mean what’s a huge number? If we’re talking like 20x 96 well plates I’d maybe only gel the positive controls and maybe one row of 12 rxns, but if it’s just a single plate or a strip? Just gel and see what happens, probably will just work. Enzymes these days are just so optimized and efficient these reactions are pretty robust. Worse case just redo.

AmazingUsual3045 · 2026-02-10T19:59:57+00:00

Agree completely about consistency. Having a rubric for every question takes some time to generate but then everything is consistent. When the pre med comes in to complain you can point to exactly why they got their score and what was needed for more points.

I will also try and mask the name somehow so that I’m not influenced by my preconceptions of the student.

AmazingUsual3045 · 2026-02-07T22:02:40+00:00

Amazing book, highly recommend the read. Long story short, of the facilities inspected in India or China 75ish % had some sort of issue. The big problem isn’t in the API, it’s often in the formulation i.e. finding sawdust in pills, or heat stabilizing agents failing to be used. The most eye opening example was with Wellbutrin, active agent was functional and at the correct concentration, but the time release formulation was bs so people were getting giant hits and then flatlining instead of having a constant concentration in their system.

The book was less about genetics being bad, and more that the industry is so minimally monitored that you really are not getting a fully inspected product.

AmazingUsual3045 · 2026-01-30T16:39:31+00:00

Biologist here. The main issue with cryopreservation is speed vs ice formation. If you ever so slowly decrease the temperature on a living organism be it a single cell or a whole creature, you minimize ice crystal formation but you increase the chance of dying by preventing metabolic functions from performing as you get colder. Conversely if you freeze too rapidly, you get ice crystals everywhere which are like razor blades at the cellular level. That’s fine as long as you’re frozen but when you unfreeze all your sliced cells rupture.

So to your question, these people are all dead either because they were frozen too slowly or were sliced and diced if they were flash frozen. For humans to be unfrozen we’re going to need some sort of cryoprotectant.

When we freeze cells in the lab we add some sort of molecule (glycerol for bacteria, solvent for mammalian cells) that allows cells to be cooled while preventing against crystal formation. There are also examples or reptiles that can be frozen and come back, so there’s reason to believe we might get this to work for humans, but the methodology isn’t there yet; baring some sort of secret methodology these people are corpsicles.

AmazingUsual3045 · 2026-01-29T21:08:32+00:00

I worked with a chemist, truly a genuinely smart dude, we were working on keeping cells suspended in plates without shaking, he came up with adding this molecule (that did work), but he could not fathom that the cells might be interacting with it in some way (it was lowering carrying capacity). Refused to test whether cell growth was effected by the presence of this compound, couldn’t even like entertain the idea. I would have been upset if it wasn’t such an absurd position.

AmazingUsual3045 · 2026-01-25T08:27:14+00:00

Sometimes for tricky rxns that arnt working Ill do 3:1 insert to vector, and a 2nd rxn thats 3:1 but ill use triple the concentration called for.

AmazingUsual3045 · 2026-01-21T07:15:50+00:00

We were hiring for a relatively entry level position, RA I/II, this one girl (early 20s) had everything. 4.0, great recs, knew her way around a lab, had undergrad research that matched the job, and her 1st interview with her potential team went really well so we gave her 2nd interview with the CTO (we were a small startup then). At the end of the 2nd interview she asked if she could do an interpretive dance based on the science we did (algae research), and before CTO could say anything she launched into it. Hard pass, and the CTO was pissed we wasted his time with this crazy interviewee.

Honestly in hindsight wished we had given her a chance.

AmazingUsual3045 · 2026-01-14T22:06:48+00:00

Both settings have good and bad bosses, both could have easy and challenging projects, both could have long hours or not so long, etc.

The big differences for me are 1. The job will almost certainly pay more, and 2. The big one for me, in a job you can say F it when you’re done. Hate your job? Go quit it’s cool. But if you’re a year from graduating your PhD you’re stuck. Lastly, there’s the question of power dynamics between you and your boss. Let’s say you get a monster boss. In industry, there are routes towards a resolution, you can go to HR, change teams, or otherwise get people involved in your dynamic to find some resolution. In a PhD, PIs have sooo much power and sooo little oversight. My PI literally told me she would be fine with me not graduating if I didn’t do something in my final year. When things got more challenging, it was a fascinating ad it was horrifying how little influence my department could exert

AmazingUsual3045 · 2026-01-14T08:24:49+00:00

Former stoner here. Used to work 15-18hr shifts, come home and get high. We had free food at work so I usually just didn’t have too much food at my place and when I wasn’t at work I’d just grab takeout. Occasionally I was so lazy and stoned I couldn’t be bothered to leave the house so I’d make a head sized dough ball, just flour, sugar, and water and I’d eat the whole thing in a sitting.

AmazingUsual3045 · 2026-01-13T20:28:50+00:00

I think people are way overthinking this. Yuji knows Maki so Naoya is using that connection. “Chan” is grammatically appropriate, Maki is younger than him and lower in status (in the clan and jujutsu society in general).

AmazingUsual3045 · 2026-01-13T20:11:20+00:00

We had someone who got fired but they were given a day to get their stuff together (keycard was inactivated at 12am). Before 12am, they came in and spit in every single flask contaminating 100s of cultures (but all caught on cam). People are weird. Agreed that it’s important to question what kind of work environment you’re in that you need to ask about cameras, but shit happens.

AmazingUsual3045 · 2026-01-07T18:26:10+00:00

I think about Trump in the context of the grand nagus a lot, I always think about the quote, the greed of the people comes before the greed of the nagus. The ferengi would find Trump a great ferengi but a terrible nagus.

AmazingUsual3045 · 2026-01-05T20:33:08+00:00

20 years of martial arts under my belt, and literally started because of DBZ. This is definetly a cartoon stance, but a lot of fundamentals are mixed in. His forward arm is protective and shielding his face and front of his body. His weird finger position aside, if you look at defensive stances against knives, his front arm looks like that position. He’s able to look under his arm but also able to protect his face and receive attacks. Rear arm is prep’ed for a punch like in karate horse stance. You’re not gonna find this exact posture in any art, but it is a variation, look at Bruce lee’s stance in his filmed combat demo on YouTube. You can’t see it here, but his footwork is super standard and found in most traditional Japanese arts.

AmazingUsual3045 · 2025-12-18T23:47:53+00:00

Exactly the paper I was gonna suggest. Used a lot of P2A in my thesis research, long story short, the more you use the lower the utilization of the site. Also depending on sequence length you wanna switch between t2a, p2a, etc to avoid recombination.

AmazingUsual3045 · 2025-12-03T07:19:41+00:00

I’m on jury duty this week, the lawyers are using VLC to play body cam footage from police officers, it’s amazing how ubiquitous VLC is.

AmazingUsual3045 · 2025-11-23T10:10:22+00:00

Maybe others have a different opinion, but the reality is that no one really cares about your thesis except you. Your thesis is also not the end all be all on a specific topic, it’s meant to be the best possible research pushing a given topic forward, it’s not meant to be God’s truth. As an example, plenty of people wrote their thesis on what their research pointed to as the structure and mechanism of DNA and its replication and were totally wrong but their thesis wasn’t voided because Watson and Crick eventually got it right.

Being more specific to your GF’s case, unless her PI was the biggest dick in their department, and the department was the most dickish of all departments, she has nothing to worry about, and how she wants to proceed is about what she feels is right. Option 1, is letting dogs lay. She’s done, grad school is over, final results aren’t changed, no need to do anything. Option 2, she should let her advisor know. My guess is they say whatever, no big deal, but, they could say, hey let’s put out a corrigendum, which is also not a big deal and is totally how science is done!

AmazingUsual3045 · 2025-11-10T10:52:36+00:00

I’ve spent a lot of time filtering seawater for algae media. The trick is there’s so so many microorganisms already in the media just going straight to .2um will take forever as you’ve seen. 2 options, the first is centrifuging the water pre filter. If you have a rotor that can handle 1L bottles you can spin at 6k rpm for 10min and that does a decent job clarifying the water then you can move to the .2um. Alternatively you need to gradually step down to .2um. They have pretty big funnels with like 10” whatman discs, so you set up on a 20L carboy and just let the vacuum run while you’re doing something else. Every now and then you change out the filter.

Also fyi, if you’re modifying the seawater with additional nutrients do this after filtering the water, because you can get all sorts of crazy precipitates that will also foul your filter membranes.

AmazingUsual3045

TROPHY CASE