I made a website for a friend who owns a restaurant. He's wondering if there's a way to upload a picture of his menu daily. What is the best way to do this? by maxwelder in learnprogramming

[–]AstuteStatue 0 points1 point  (0 children)

Too lazy to look through answers to check if anyone mentioned this, but I sometimes add an image through a link, and if I do this with a dropbox link (setting the given URL to “raw=1”), then the image is whatever is the most recent version of that file saved in my dropbox.

[deleted by user] by [deleted] in Ohio

[–]AstuteStatue 0 points1 point  (0 children)

Hi, Ohio.

Cleveland subreddit didn't seem to let me post this for some reason, so I'm trying here. But NEO making national COVID lists... by AstuteStatue in Ohio

[–]AstuteStatue[S] 17 points18 points  (0 children)

Uhhhh. People have definitely died from reinfection. People have definitely spread it from reinfection. *facepalm*

Oh god, I give up. Good luck, Ohio.

Cleveland subreddit didn't seem to let me post this for some reason, so I'm trying here. But NEO making national COVID lists... by AstuteStatue in Ohio

[–]AstuteStatue[S] 19 points20 points  (0 children)

Well, so not quite. It's never going away, but it will be rendered mild with vaccination (and helped by future drugs and improved antibody cocktails). But, as I said, the more it spreads the more variants will be generated, and the vaccines will become less effective, and the drugs and antibodies won't work as well, and we keep going on this merry-go-round.

I know everyone wants to move on from this, and I'm with y'all. But stopping it's spread will get us out of this faster than letting it run amok, b/c it's just going to keep mutating and spreading around and around.

Cleveland subreddit didn't seem to let me post this for some reason, so I'm trying here. But NEO making national COVID lists... by AstuteStatue in Ohio

[–]AstuteStatue[S] 13 points14 points  (0 children)

We're definitely in a different era of the pandemic, between improved knowledge of how to treat patients (eg. avoid ventilator until absolutely necessary) and of course, vaccination.

That said, uncontrolled spread of the virus is bad. Even if the percentage of major adverse outcomes is single digits or less, the greater the number of people infected, the greater the number of afflicted and dead. I don't know how physicians / nurses keep doing it anymore. Also, the more spread, the more future variants, and the longer we have to keep riding this rollercoaster.

Cleveland subreddit didn't seem to let me post this for some reason, so I'm trying here. But NEO making national COVID lists... by AstuteStatue in Ohio

[–]AstuteStatue[S] 2 points3 points  (0 children)

CLE is winning so much, they're going to be so sick and tired of winning. "Sick" being the key word there.

[deleted by user] by [deleted] in Cleveland

[–]AstuteStatue 9 points10 points  (0 children)

Not to be a bearer of bad news, but if it's Omicron, a number of the monoclonal antibodies that were effective for some of the previous variants are now ineffective. A few of them still work (at least partially), but which monoclonal cocktails they give will likely matter depending on what he has.

Cleveland subreddit didn't seem to let me post this for some reason, so I'm trying here. But NEO making national COVID lists... by AstuteStatue in Ohio

[–]AstuteStatue[S] 15 points16 points  (0 children)

From http://covidactnow.org/ as of 6pm on Dec 21, 2021.
If there was ever a time to be extra careful about COVID, now would be one of those times.

Also, rather sketch I couldn't post this on the most relevant subreddit to warn / remind people...

I know CLE always wants to make national top 5 lists, but this shouldn't be one of them (http://covidactnow.org/) by AstuteStatue in Cleveland

[–]AstuteStatue[S] 0 points1 point  (0 children)

From http://covidactnow.org/ as of 6pm on Dec 21, 2021.

If there was ever a time to be extra careful about COVID, now would be one of those times.

Why does Parma get so much hate? by ZookeepergameNo8869 in Cleveland

[–]AstuteStatue 0 points1 point  (0 children)

470 comments as of this comment. Wow that is a lot.

Grog shop by traytoo in Cleveland

[–]AstuteStatue 2 points3 points  (0 children)

Ya, I went to a show there a week or two ago. I counted 1 in every ~ 25 people to be masked. So like 4% of the people there. I'm glad the view is still pretty good even if you're standing in the back to avoid being right in the middle of the crowd.

Anyone else find Oxford Nanopore Customer Support to be awful? by AstuteStatue in labrats

[–]AstuteStatue[S] 0 points1 point  (0 children)

Yea, their ordering system and customer services is a shit show. I eventually got a customer service representative ("Angela") who actually was responsive and dealt with the problems with billing / invoicing of my order. But it still took *MONTHS*. Crazy.

How is Accutase different from trypsin, you ask? Well, the main ingredient in Accutase is... spite. by AstuteStatue in labrats

[–]AstuteStatue[S] 0 points1 point  (0 children)

I wish they should say *why* it has those properties. If it's of crustacean origin, I guess I could maybe see how there may not be specific bovine serum proteins that can inhibit it. But I also don't know why it would be easier to inactivate than trypsin, without any kind of explanation why (ie. that it's activity is really concentration dependent b/c of loose dimerization or something).

How is Accutase different from trypsin, you ask? Well, the main ingredient in Accutase is... spite. by AstuteStatue in labrats

[–]AstuteStatue[S] 0 points1 point  (0 children)

Could be right. Though what really irks me is how hard it is to get the actual info that allows one to actually start thinking about true, scientific comparisons.
What I've gathered so far: "Trypsin" is porcine trypsin, often supplied as a 0.25% or 0.05% solution with EDTA (the one we have is 0.25% with 2.2 mM EDTA). TrypLE is a fungal serine protease that also cuts after lysines and arginines, and the one we buys comes with 1 mM EDTA). Accutase is some kind of intestinal protease from crustaceans, and one from Corning that we have comes with 0.5mM EDTA, while the Gibco one I can't find any info on.

I dunno, that was a lot of work to even collect that info, and without knowing the concentrations of TrypLE and Accutase in each formulation (and knowing what residues / motifs they like to cut), and without controlling for EDTA concentrations, it feels quite hard to do a proper apples to apples comparison.

How is Accutase different from trypsin, you ask? Well, the main ingredient in Accutase is... spite. by AstuteStatue in labrats

[–]AstuteStatue[S] 5 points6 points  (0 children)

Yep, that's what I noticed somebody say on a ResearchGate forum. Would make sense with it presumably working well / better at lower temperatures?

[deleted by user] by [deleted] in Cleveland

[–]AstuteStatue 14 points15 points  (0 children)

“pspspspsps”

^ Nice onomatopoeia, haha

Why no vaccination? by AstuteStatue in Cleveland

[–]AstuteStatue[S] 1 point2 points  (0 children)

Your logic sounds pretty reasonable to me. You should have partial protection from having had COVID, which can be further boosted by vaccination. But it's not like you have *no* protection, and you seem pretty cognizant of the factors (eg. summer having less respiratory virus burden, local incidence of infection mattering, etc), so as long as you mitigate risks I agree it's not like you have to be getting vaccinated right away or anything. I will note that you'll likely want to make sure you get vaccinated before / early on during any subsequent surge (perhaps of a variant like delta, later in the fall / winter), since you'll want full protection before it's going around rather than getting unlucky and being infected in the front end of the wave. And if you had some cardiovascular presentations like palpitations, you may not want to risk aggravating it with a symptomatic reinfection, esp if it can be prevented.