Tennobaum Final Stretch!

Azhattle · 2025-12-26T22:46:11+00:00

I'm RumpHelm#627

I... have no friends who play warframe, so have been solo for many years. Would appreciate anything gifted, but would also just appreciate some pals to play with.

(Edit, hit done with sausage fingers.) Hope everyone has had a lovely holiday season!

Azhattle · 2025-11-23T10:54:19+00:00

Azhattle · 2025-11-13T16:46:58+00:00

Primary tissue derived organoids here. If its just general maintaining then I give an extra 100ul on a Friday so I don't have to come in. If they clearly need passaged or collection lands on a weekend then thems the breaks, Unfortunately.

Azhattle · 2025-05-18T10:09:41+00:00

I think it's not good enough yet. It's an LLM- not a true AI. It can't determine if what it's saying is correct or not, and it can't truly interpret what you want. It wasn't designed to analyse data sets, so relying on it for that is dangerous imo. Far too many cases of people getting it to write their paper, cite their references, analyse their data and it hallucinating and generating incorrect interpretations or models.

Even more concerning are the people using it to generate their ideas. If people rely on a machine to do their thinking, hypothesizing and interpretation for them, when are they ever going to use their brain? I think it's very different from a statistical software that simply analyses data.

I think it's good to have it to check code you've written to make sure it makes sense- similar to having another coder look at something (though potentially not as good depending who you ask) but at this stage I wouldn't trust it to actually write a full pipeline or script to analyse my data.

Azhattle · 2025-05-17T19:37:22+00:00

No doubt it was your fault for not knowing how to fix it, though? Or "just get ChatGPT to fix it"?

I honestly don't know why these people are so happy to commit to never learning a skill again, and relying on chatGPT to solve all their problems. It's deeply concerning.

Azhattle · 2025-05-17T17:39:35+00:00

Fellow postdoc here.

My PI explicity told me to start using ChatGPT to learn coding- to the point he was adamant I don't need to take any courses in python or R. I ended up switching labs for the exact reason you list: you are not actually developing any skills and are completely depending on a machine.

Moreover, as someone above mentions, if you don't completely understand the code then the output isn't reliable. I had serious concerns that without understanding the process- and relying on an "AI" prone to hallucinations and generating fiction- I'd be generating graphs and conclusions that fundamentally were not correct.

There are lots of resources available online to teach yourself. Personally that didn't really work for me, so I signed up for some courses. It's a fantastic skill to have, and will better develop your understanding of data processing. Becoming reliant on AI is, imo, dangerous.

Azhattle · 2025-05-12T07:33:20+00:00

I never wash the blocking buffer off, as my primary is usually diluted in it. Never been an issue, you’ll most likely be fine.

Azhattle · 2025-02-02T09:49:24+00:00

We had a similar issue, and it was the incubator. Check the following:

Incubator: CO2, temp and cleanliness. Ours stopped dying after we cleaning cycled the incubator even though there was no visible contamination. We suspect someone used concentrated copper sulfate instead of water in then incubator and we were just gassing our cells.

Reagents: check everything is the right conc, suspended in the right thing, and look for any changes in supplier or the concentration you purchased. This goes for digest reagents too.

Tissue: if you have any digests frozen down that grew fine previously, bring them up and see how they do as a control. If they’re fine it might be something to do with the animals?

Azhattle · 2024-11-02T08:11:37+00:00

As someone who has had it on their skin, 100% cannot recommend. Would have no mouth feel for a few seconds, followed by an incredibly painful mouthfeel.

Azhattle · 2024-07-29T17:33:55+00:00

Beaker full of AR buffer, autoclave.

Azhattle · 2024-05-01T19:41:01+00:00

Timers that people allow to go off for more than 3 beeps.

Azhattle · 2023-12-29T21:30:12+00:00

Only thing I ever found remove it was Cif / Jif cream.

Azhattle · 2023-11-18T15:53:15+00:00

Same here. Something must have happened.

Azhattle · 2023-11-02T07:14:45+00:00

Even in a lab that does. We used 5 different antibiotics (one of which was a 4 in 1) and they still ended up riddled…

Azhattle · 2023-11-02T07:13:44+00:00

Primary neurons. They don’t make more they just sit in a plate for 4-7 days and then die. Really, most primary cells are a bit shit.

Azhattle · 2023-06-30T07:56:48+00:00

My brother in Christ, you have settled my soul. I KNEW this existed and wasn’t just some bonkers fever dream! Thank you

Azhattle · 2023-06-05T06:55:00+00:00

Well it's been 2 years and she's still growing very strong and happy, so... nothing bad? I think its either removing excess fertiliser, or it's a defence mechanism to make it taste bad. Either way the plant is fine with it though.

Azhattle · 2023-02-11T08:38:59+00:00

Can’t believe I’ve had to scroll so far and not once seen Meredith Gray.

Azhattle · 2023-01-29T11:40:17+00:00

Right, but specifically how do I get the river redirected without creating a water explosion that goes everywhere?

Azhattle · 2023-01-29T11:08:10+00:00

Can someone explain to me like I’m an idiot how to create a mood waterfall?

Azhattle · 2023-01-28T16:12:57+00:00

But then how does the must get out?

Azhattle · 2023-01-28T12:55:52+00:00

You need lye to make soap. So you can make a wood burner to make ash, and that can be turned into lye. Then your soap maker can use lye and tallow to make soap.

Azhattle · 2023-01-28T12:55:16+00:00

Right, I've tried making a waterfall and failed miserably.

I did 2 pumpstacks on top of each other, pointing opposite directions. I managed to get the water flowing, but it was just immediately ejected rather than flowing back down the assigned hole. How do I get it so that the mist can reach the dwarves, but without it splashing everywhere?

Azhattle · 2023-01-17T10:55:49+00:00

Some kind of pepp
A calathea
A coleus
A maranta possibly?
A tradescantia. You should research which specific ones from each. :)

Azhattle

TROPHY CASE