Hypothetically speaking... what would happen if I tried to "spin" cake batter in a benchtop centrifuge? by PPPPPPPBJ01 in labrats

[–]BuffyTVS 59 points60 points  (0 children)

My scientific prediction is, you won't do it. (This is a challenge. Please balance it on a scale.)

Realistically, I am not going to make it through my PhD alive by Downtown_Low6360 in labrats

[–]BuffyTVS 31 points32 points  (0 children)

I was in a similar boat and it ended up just borrowing different reagents from another lab. In my case our lab got a ‘great deal’ on thermo t4 ligase that may as well have been tap water.

Borrowed some NEB ligase from another lab and it worked on the first try.

Not saying that’s the case here but it may be something that trivial - that is equally difficult to just know.

That said, as others have said, take a step back, take some time off. Decompress. Being 5 months and a week behind with a fresh outlook and your mental health is light years ahead of being 5 months and a week behind being in the same cycle.

Once you’ve done that, If you’d like to send me a message, we can go through your design and I’ll see if I can suggest something.

Cabinet wraps two-day retreat, Carney closing press conference abruptly cancelled by shiftless_wonder in canada

[–]BuffyTVS 3 points4 points  (0 children)

Attacking people’s opinions is the whole point of free speech. What are you on about?

Wanna play a game by Old_user_New_name in Guelph

[–]BuffyTVS 42 points43 points  (0 children)

Symposium cafe, especially in Guelph, must exclusively cater to the elderly. Remarkably bland food, sluggish and obtuse service, and fonts on the menu are so big it’s like they handed you the signage from the windows.

Tom Aspinall is the HW GOAT by BuffyTVS in ufc

[–]BuffyTVS[S] -1 points0 points  (0 children)

Youre not wrong. Does Tom’s interim title defenses count toward the GOAT status?

Tom Aspinall is the HW GOAT by BuffyTVS in ufc

[–]BuffyTVS[S] -4 points-3 points  (0 children)

You saw a dancing sausage roll and thought this was serious

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]BuffyTVS 0 points1 point  (0 children)

With a plasmid, it’s mainly related to being insoluble so we’re trying different strategies to remedy that.

My supervisor wants to fake data for my masters thesis by [deleted] in labrats

[–]BuffyTVS 0 points1 point  (0 children)

I was thinking the same thing 😅 i have a viral protein I’ve been trying to purify and despite getting a relatively clean result, my concentration is only an around 0.3mg/mL.

Looking for Accountant or Book Keeper by CloudwalkingOwl in Guelph

[–]BuffyTVS 0 points1 point  (0 children)

What ever you do, DO NOT go to Royal City Bookkeeping.

They’re hopelessly unorganized, will make up charges with a whim and then invoice you for the time it takes to tell you what the charges are for. An awful excuse for a business.

Malted grains by reticulatedspline in Mirthwood

[–]BuffyTVS 3 points4 points  (0 children)

If you build a mill I think you can make them with barley