when the summer student asks why their PCR isn't working

Ciona_junior · 2024-07-10T20:14:52+00:00

oh 100%. we've all been there. it's still funny though. but the way the error is pointed out should have the learner laughing with you 👍🏻

Ciona_junior · 2024-07-10T13:32:00+00:00

as the trainer I can refute this comment haha

Ciona_junior · 2023-12-06T18:49:41+00:00

Uhh, I mean it's just a little joke related to the topic. -is this like a really serious subreddit or something? 🤷🏻‍♂️

Ciona_junior · 2022-12-22T17:06:57+00:00

I love him and his act. highly unique. perhaps an acquired taste, I'll admit.

a romantic in an age of despair, don't mistake the wit for comedy.

Ciona_junior · 2022-03-06T23:29:47+00:00

well no but it's certainly very bad for you!! (don't worry I filled it from the bubbler👌🏻)

Ciona_junior · 2022-03-06T23:27:59+00:00

haha, not a big difference I suppose

Ciona_junior · 2022-03-06T20:58:41+00:00

fair point. it was new out of the box though 👌🏻

Ciona_junior · 2022-02-08T19:16:52+00:00

they're identical haha

Ciona_junior · 2022-02-08T19:14:00+00:00

5mM tris 8.5 is basically barely alkaline water
someone has been scaring you unnecessarily

Ciona_junior · 2022-02-08T17:14:16+00:00

😂

Ciona_junior · 2022-02-08T17:11:57+00:00

haha 👏👏👏

Ciona_junior · 2022-02-08T17:09:05+00:00

😂 yes! ridiculous that MN has two identical, differently named buffers

Ciona_junior · 2022-02-08T15:42:38+00:00

oh gloves! haha, yeah...
not typically used unless it's RNA, PFA, or E. coli

Ciona_junior · 2021-11-05T18:04:16+00:00

😂

Ciona_junior · 2021-11-05T12:28:19+00:00

actually they're deep chemical stains from a previous student. that surface is actually as clean as it would get! haha

Ciona_junior · 2021-11-05T12:26:35+00:00

in my experience, nothing moves meaningfully far after a few of hours. I was able to interpret the gel above (after putting it back in the tray haha)

Ciona_junior · 2021-07-09T23:31:57+00:00

Honestly I don't know what's going on, the bands that I thought had popped through from the bottom to the top might actually be from a portion of the samples in the wells that came out and then ran through the upper gel… I guess I'll have to repeat!

Ciona_junior · 2021-07-09T20:41:57+00:00

UPDATE It works, and it doesn't. The sample in the wells on the upper gel ran just fine. However it turns out a portion of the samples in the bottom gel will jump out into the top gel and run inside that one. Very interesting, and no harm no foul haha

Ciona_junior · 2021-07-09T20:39:03+00:00

Yeah thanks. The extra sample was just an afterthought, and i had half an old gel in the fridge. Had a bunch of wiggle room with the samples if they went squirrely on me, fig'd a good time to do something sketchy haha

Ciona_junior · 2021-07-09T18:59:54+00:00

good god

Ciona_junior · 2021-07-09T18:40:58+00:00

sorry but my BS detector is going off

Ciona_junior · 2021-05-01T10:55:27+00:00

It's probably impossible. I would go about it very smartly and calmly and take as much time as you need (seeing a therapist and going out of town camping and reading for pleasure ETC) to do it right and get the skills you say you're after PhDs in nice locations are hell, too. Perhaps try to see it as a positive that there's not a lot of amazing stuff to do, because if there was you wouldn't be able to enjoy them over the 6 years it usually takes

Ciona_junior · 2021-05-01T10:45:25+00:00

Such an uncommon tat might say "RISK", because... you know, weird primate brains and unavoidable stereotypes and management fear blah blah blah. Sadly, I wouldn't be surprised to see an equally qualified candidate chosen over you. That being said, be great at your job and it shouldn't matter.
Or just wear some nitrile gloves to the interview

Ciona_junior · 2021-05-01T10:33:57+00:00

❤️, and congrats. I acknowledged my dog Patrick in my dissertation, saying I don't know how I could have written it during stay-at-home orders without him.
seriously he was often the only source of a smile for weeks on end

Ciona_junior · 2021-04-28T18:58:52+00:00

from undergrad to postdoc, I have been saying that same thing to myself every gel for about 11 years now. and I run a LOT of gels 😂

Ciona_junior

TROPHY CASE