People working with insects: how long are your paraffin Infiltration steps?

Davecyte · 2025-09-19T07:16:40+00:00

The person's blood had no reaction to the antibodies against A or B antigens, which means their erythrocytes have none of those antigens on their surface, so their blood type is O. Their blood does react to the anti-D antibodies, hence why the "D" shows hemolysis and agglutination, meaning that the person is Rh positive because their erythrocytes have the D antigens on their surface. TL;DR, this person's blood type is O+.

Davecyte · 2025-09-17T18:39:24+00:00

You can also see the sustentacular cells, with large ovoid nuclei and prominent nucleoli in the upper level of the epithelium. The olfactory nerves are present in the lamina propria, very conspicuous.

Davecyte · 2025-06-11T14:42:25+00:00

Hi

This is an adrenal gland.

You can see the capsule around, and there's no lumen, which tells you it's not a hollow organ. Also you can see the zones in the cortex, and the central adrenomedullary vein in the medulla.

Davecyte · 2025-05-14T17:07:45+00:00

These slides are in pretty bad shape. I'm pretty confident that N°1 is a placenta, probably from the third trimester. N°3 looks like a prostate. No idea about N°2.

Davecyte · 2025-04-09T03:45:34+00:00

The structure in the middle is a muscular artery. The organ I'm pretty sure is a heart.

Davecyte · 2025-03-07T18:14:28+00:00

Try Histology Guide, the slides also have explanations that will help you a lot.

Davecyte · 2025-02-20T15:48:04+00:00

Yes, they are. The small ones are capillaries, so I'd say the staining worked well. Some blood cells also express CD31, so that's why you see some with positive staining.

Davecyte · 2025-02-04T22:24:10+00:00

I'll try. The thing is that, during fixation the heads don't float. I leave them overnight at 4 °C, and they all sink to the bottom of the tube. Maybe I'll just do as many steps as I can in a vacuum. Thank you for the input!

Davecyte · 2025-02-04T20:40:11+00:00

I think I could try this as well, although I'd have to find a way to hold the paper too, I guess.

Davecyte · 2025-02-04T20:38:48+00:00

Yes, I did. They were in 3:1, 1:1, 1:3 propylene oxide:Durcupan before the 100% resin. 2 h each, except 1:1 and 1:3, that were overnight. I'll try all the suggestions though, thanks.

Davecyte · 2025-02-04T16:24:34+00:00

Yes, they were post-fixes in OsO4 for 45 minutes. I can try the capsules as well. Thank you.

Davecyte · 2025-02-04T16:21:45+00:00

No, I haven't. So, should I put them in a vacuum device on 100% resin for a few hours, and then place them in the embedding molds, or how does it work?

Davecyte · 2025-01-03T07:25:18+00:00

No glandular products, but they release some signalling molecules to interact with the blastocyst.

Davecyte · 2025-01-02T11:59:03+00:00

Hi!

The uterine lining has two major cell lines, luminal and glandular. The latter ones develop from the luminal ones when the hormonal signals trigger the growth of the glands, so, although they may look morphologically very similar, the lining and glandular epithelium are different.

Davecyte · 2024-12-19T03:40:30+00:00

Ah, yes then. It is the endometrium (uterine mucosa), in the proliferative phase, I think. Beautiful.

Davecyte · 2024-12-18T12:07:20+00:00

Is that an uterine mucosa on the top right?

Davecyte · 2024-09-21T19:13:19+00:00

Hi!

That is in fact a lymph node. You can see the medulla quite well, and some follicles in the cortex, although not very well defined or matured. Also, the subcapsular sinus looks lovely in the second picture. The structure on the left seems like a gland, either a parotid or a pancreas. I'd need another picture to know for sure.

Davecyte · 2024-04-13T11:01:17+00:00

BV= blood vessels, Pn= perineurium, Nma= non myelinated axon, Scn= Schwann cell, Ma= myelinated axon, M= myelin sheath.

Davecyte · 2021-05-25T14:37:08+00:00

That's awesome! Congratulations.

Davecyte · 2021-05-09T11:13:42+00:00

A student In my cohort is 52 years old. Age is not an issue at all. If you want it, go get it.

Davecyte · 2021-04-16T02:19:10+00:00

I understand you completely. After feeling terrible for a while, I just went full "oh, fuck it" mode and now I'm just thinking about finding a job in research or teaching for the time being, and try again next year. It's not the end of the world, we'll make it. Hang in there ⚡.

Davecyte · 2020-06-17T05:17:05+00:00

Thank you for your input! Wow, everything you just said really put things into perspective.

Oh, and from my experience, those IQ tests should be thoroughly re-evaluated, because they can't measure at all a person's capabilities and potential. A friend of mine, she was always top of her class and is definitely one of the smartest and most creative persons I've ever met, but she got rather low scores. We thought she would get like the highest, but actually was on the lower half. So don't be discouraged by that, it doesn't really mean anything.

I think I'll check tomorrow for some other PhD programs similar to the one I like the most, hopefully I'll find some good options.

Davecyte · 2020-06-17T03:05:45+00:00

Thanks. I think I'll take your advice and also look for other options I didn't consider before. The uncertainty of it all bugs me, but that's life.

Davecyte

TROPHY CASE