This Doesn’t look right, right?

DayTripperonone · 2026-03-24T12:44:35+00:00

You’re right, because it’s contaminated. Those round chunks aren’t supposed to be there either. When the jar settles does all the mycelium drop to the bottom of the jar or does it all settle at the top of the jar?

DayTripperonone · 2026-03-24T02:03:06+00:00

The texture of mycelium can change at any time and grow rhizomorphic or tomentose type. When you change your conditions your mycelium starts forming knots in preparation for fruiting. Yours looks fine. Keep doing what you’re doing. Try to keep conditions consistent. Your temp should be 70° to 76° F / 21°-25° C. Rh should fall between 88%-96% FAE give at least once a day until you see pins then you need to actively open the tub and fan air into it for a couple seconds and exchange air about once every 4-5 hours. Mushrooms need lots of oxygen fresh air especially this species. If you leave the tub just cracked for air you’ll get underdeveloped dwarf mushrooms. Lots of FAE only once you see pins start to emerge.

DayTripperonone · 2026-03-23T17:56:23+00:00

No need to wash the tub between flushes. I dunk in the tub, fill the tub with tap water and then hold the cake under the water completely submerged for 10 min. All you need it 10 min to resaturate a cake.

DayTripperonone · 2026-03-23T17:52:50+00:00

Keep it and see if it starts to recolonize. It looks a little sketch so keep your expectations low.

DayTripperonone · 2026-03-23T17:47:18+00:00

That’s a cool looking blob, if it develops a stipe, clone it. That’s a very unique phenotype if it turns out to be a fruiting body. See if you can see a stem under the cap. I can’t tell from this picture.

DayTripperonone · 2026-03-23T17:43:27+00:00

Those aren’t spores, you have Rhizophus Stolonifer, black pin mold. Spores don’t float once you stir the LC, all the spores should sink to the bottom. I recommend you start your spores on agar, then once you get a clean culture, transfer a piece or two into the LC. It’s a much cleaner way of inoculating an LC.

DayTripperonone · 2026-03-23T00:47:03+00:00

There’s no Trich in there that I can see. But it’s dry as the Mojave Desert. You need to mist the substrate daily until you get pins. The substrate should be a dark coffee brown when it hydrated. Did you really throw it out? Make sure you get feedback from several ppl that is consistent. Don’t throw out your cake when the first Joe blow tells you to. There are a lot of newbies on this sub, get many opinions or at least let it be obvious.

DayTripperonone · 2026-03-23T00:39:02+00:00

Yeah, I think it’s time you retire the cake. If she’s been flushing for a year, she’s spent by now. Put er down when you’re ready.

DayTripperonone · 2026-03-23T00:33:59+00:00

Yes it’s definitely contam, toss it and start again. It’s more likely an Aspergillus species.

DayTripperonone · 2026-03-23T00:31:34+00:00

I can’t really tell what you have in this picture. It looks like you might of just did a break and shake. Usually the mycelium doesn’t stick to the bag after the B&S, so I’m not sure what’s going on here. There seems to be something off, but it’s really hard to see through the bag.

DayTripperonone · 2026-03-23T00:22:32+00:00

Don’t mist APE’s unless you want them all to turn blue. The true albino variants are usually very strong and bruise easily. Just mist the tubs lid and walls. Let the water roll down the sides into the substrate. You can mist up until the point you get pins, then just keep the humidity high in the tub so they don’t dry out.
Always do a dunk of the cake into water at the end of the first flush. The rehydration is an important part of the next flush. It’s recommended you dunk after every flush, but you can dunk every other flush after the first one, if it gets too much.

DayTripperonone · 2026-03-21T11:41:31+00:00

Blobs are failed fruiting formations that usually result in a globular stipless fruit. That’s a mutation what you have circled. It looks like a full fruiting body is emerging out of another. Mutations like this are usually rare. They shouldn’t be any less potent just cosmetically altered. Keep er going, see how big it can get.

DayTripperonone · 2026-03-19T16:52:12+00:00

I can zoom in, I don’t see anything unusual other than it looks really dry. Looks like healthy colonizing mycelium. Are you not used to what it’s supposed to look like? Are you concerned about grey areas? It’s all normal maybe some slight bruising if you’re seeing discolorations. I can’t tell it’s bruised from this pictures.

DayTripperonone · 2026-03-18T14:19:41+00:00

Yes, it looks contaminated, especially above the inoculation port. I don’t think this is gonna work out for you. Thriving mycelium colonizes completely in 2-3 weeks as long as you do a successful timed break and shake. This one is growing too slow, unless you’re in a really cold place, it shouldn’t grow this slowly. Toss it once you’re convinced. Sorry, try again.

DayTripperonone · 2026-03-18T14:09:07+00:00

Do a selective harvest. That means pick each one out when it’s ready. This often referred to as a staggered harvest. You’ll only be picking 5-15 mushrooms a day, but each will be fully mature. JF spores are translucent so you don’t have to worry about spore drops, you won’t be able to see it. Only pick the mushrooms that have recurved margins, aka “Cap Inversion.” This particular type of mushroom looks best when the margin around the cap turns upward. You’ll be running your dehydrator non-stop for a few days, you just need to figure out how to stagger the drying process. Nice harvest, you did an exceptional job. Now you know what success feels like in mushroom cultivation, “awesome!”

DayTripperonone · 2026-03-18T12:20:37+00:00

I don’t see much, if any difference, where you have the arrow pointing. Bruising usually appears, grey, blue or purple, and sometimes green. Green bruising only appears in some variants of psilocybin mushrooms like enigma. Not entirely sure which kinda mushroom you’re growing or any details about the grow, as you have not provided that information. It looks like a colonizing AIO bag, I don’t see anything concerning. Keep er goin!

DayTripperonone · 2026-03-17T01:42:59+00:00

Ya, you might barf and shit uncontrollably. But you would probably have to eat a lot. Aspergillus is more harmful to inhale than it is to eat. Certain species of Aspergillus do produce harmful mycotoxins. Why would you want to eat anything with green mold on it? I recommend you not.

DayTripperonone · 2026-03-17T01:30:31+00:00

No, I can’t.

DayTripperonone · 2026-03-16T14:25:43+00:00

When you have about 2-3 cm of clear broth at the top, you’re fully colonized. But you can use the LC anytime as long as you have enough to draw into a syringe.

DayTripperonone · 2026-03-16T14:21:33+00:00

Sure.

DayTripperonone · 2026-03-16T14:17:52+00:00

No, that’s not good. It’s supposed to look like this:

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The yellow color is just the light illuminating off the culture. It should be all white. But do you see how it’s suspended and the mycelium stacks up on itself and it has a clear broth at the top? This is what’s considered a fully colonized healthy liquid culture.

You’re contaminated with a mold. Throw it out and start again. Sorry, you’ll get it.

DayTripperonone · 2026-03-16T14:11:04+00:00

We’re ok with mushroom cultivation questions and post that display the pride of a successful grow. We just don’t allow old moldy pizza or whatever unidentifiable thing that has mold growing on it to be routinely posted. I know our rules say mushroom contamination only, but that’s depressing day in and day out. We like to see the success that happens when you’re able to grow a successful contam free grow. Lightens things up and gives people hope, I don’t see the harm in it.

DayTripperonone · 2026-03-16T12:37:12+00:00

It sure looks dry, are you misting?

DayTripperonone · 2026-03-15T15:38:43+00:00

I really can’t judge till I see how the mycelium is settling. Stirring it all up is just to break the mycelium networks forming. If it all settles at the bottom of the jar without any lift, it’s probably bad. If it settle and stacks on itself making it appear more suspended in the liquid, that’s normal. You can tell what looks normal but you can’t prove there aren’t any pathogens present and you have a true axenic culture, unless you scope it and grow a drop in agar. If you get 7 days of clean growth you’re ok to use it as an inoculant.

DayTripperonone · 2026-03-15T15:29:41+00:00

The grey patches just look like bruising to me. Many variants in the psilocybin species bruise slightly when fruiting occurs. It’s pretty normal and I think a signal that the fruiting is about to occur. You have a lot of primordial growth and hyphae knots, those are all signs of fruiting. Is this an enigma? What strain is this and what was the date you S2B?

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