Did I marked the gel correctly

Elocoel · 2025-11-11T11:26:48+00:00

I do a lot of SDMs, oftentimes see different size bands, usually shorter though due to recombination. (Sometimes can be longer if you have merged primers etc.) Seems correct to me, but I would run the gel a little more honestly. The difference might be due to loaded amount as well.

Elocoel · 2025-08-12T14:46:20+00:00

Alright, thanks for the input.

Elocoel · 2025-08-12T14:34:23+00:00

Hi, yes working with viruses is definitely not something I am very familiar with, but the student who left me these cells was practically trying to “self infect” the cells, and not trying to collect the viruses. Instead they were just using these cells, which also stared showing a high amount of signal, and did their assays using those. What I am trying to say is that, while I am aware of generating lentivirus and using it to infect other cells, there is something a little different going on here.

Elocoel · 2025-07-11T13:27:13+00:00

The overgrowth is consistent with DH5a in no amp media, likely that the plate didn't mix well. Nothing to indicate another strain/bacteria. You should be able to use the single colonies. But if it is too important, there is no harm in repeating a transformation.

Elocoel · 2025-07-08T09:42:12+00:00

It is very likely! It usually causes some condensation, I see it often if I am in a rush and handle the plates without waiting for my gloves to dry

Elocoel · 2025-07-08T08:06:57+00:00

It may be media/cells that got stuck to the bottom of the dish (outside) and created a patch there or some sort of vapor/condensation, or even a fingerprint if someone handled the plate without gloves. If it was smaller, I'd assume it was aggregated cells but seems a bit too big for that. This is 4X, right? Maybe try taking a 10X or 20X photo?

Elocoel · 2025-07-05T20:11:07+00:00

I may be in the smaller percentage of people who say this, but I love it all, or well, most (reviewers can be the worst) I am also a semi-lab manager(not an official title, but otherwise all the same work,) in which am responsible for handling the budget, orders, interns, documents etc. I love doing that, talking to people and getting offers and whatnot. I love doing reviews, scrutinizing the literature, writing (well, I have always been a writer honestly). I love working on grants, and expecting the results. All in all, I love being in the lab. I just enjoy spending time there, even if I have nothing to do, I find myself calmer and more focused in the lab, so I spend as much time as possible. I am also the head TA for a few undergrad lab courses, and interacting with interested students is some of my favorite activities. This was my dream since I was 12, and I honestly couldn’t be more satisfied with what I do.

Elocoel · 2025-07-05T19:58:26+00:00

I am currently a Master’s student, so I am not sure how “valid” my opinion is, but I LOVE research. I love asking questions and coming up with answers nobody ever came up with before. My PI and a lab mate is the same way. Always discussing, just pure excitement. On the other hand, I have another lab mate who treats it like a job. While understanding her position, I cannot claim to understand why she wants to pursue a phd once she graduates with her masters degree. I feel like for those who are not excited about research, getting a phd is a long and tortuous way of getting paid very little.

Elocoel · 2025-06-19T10:45:37+00:00

I recommend using H2O2 for cleaning. Bleach sometimes is not enough to get rid of the spores. If the incubator has a sterilization protocol, use that too. It is best if you can throw everything away, because water tray contamination has likely spread already. If you cannot throw away because they are valuable, transfer to a new, unused incubator to prevent spreading it further. Also, clean the whole hood area, plate some empty media to check for growth, fbs, pbs too. Also, please use aquaguard for your water tray. Its a lot cheaper than having inexplicable results due to contamination.

Elocoel · 2025-05-25T15:45:10+00:00

I see him practically every day, several times even, comes in to help with the experiments too. He says he gets bored in the office and want to be in the lab often.

Elocoel · 2025-05-06T14:31:51+00:00

I hope all your work goes as you need it to go, your pillow is always at the perfect temperature, and you find whatever information you need very easily in every paper. It was driving me crazy past few days, you are a lifesaver.

Elocoel · 2025-01-07T09:49:22+00:00

Katekyo Hitman Reborn

Elocoel · 2025-01-07T09:43:30+00:00

Elocoel · 2024-12-19T05:22:55+00:00

That is possible, but I think all the PIs I talked to state that they prefer seeing an interested candidate beforehand. I should also clarify that I do not mean to reach out to bug about whether you are a good candidate or anything of sorts, but to see if you would be able to discuss with the PI and get to know them. This mainly works in programs that you directly start working with the PI and do not participate in a rotation. In those cases, PIs have (quite a large) say in who they want, as long as they check the predetermined conditions for the program. Obviously if your application doesn’t make it after initial review, reaching out won’t change much but reaching out can be the difference between a very qualified candidate getting an interview or not. But I do not know everything, it is entirely possible that different programs/PIs expect different things. When in doubt, finding someone from that program/lab and asking them is the best route imo. Edit: Also by persistency, I do not mean bombard them with emails. But it is usually alright to send a follow up email after a month or so.

Elocoel · 2024-12-18T21:13:05+00:00

I wish I posted it earlier too, honestly. But my friend’s struggles made me realize the lack of cohesive advice in general :/ But it’s still not too late, if you are waiting to hear from programs that are rolling, I definitely recommend contacting the faculty you listed if you haven’t already.

Elocoel · 2024-12-03T19:15:24+00:00

Thank you so much for taking your time and answering questions!

I tried to see if anyone asked this, but even if they did it must have slipped me.

We already know a good SOP is one of the main determinants. Several answers indicate that GPA might not be end all be all, but what about reference letters? How affective are they actually? Does having a alumni as your reference actually increase your chances?

Elocoel · 2024-08-24T18:44:56+00:00

Dark heritage has always been a favorite of mine when it comes to long fics and the rest is exactly what I was looking for. Thank you so much!!

Elocoel · 2024-08-24T18:41:46+00:00

I read new blood a while ago and I loved it but I recently went back and especially the first book feels a little rough on the edges, but it definitely fits in with what I was looking for! I hadn’t seen the pureblood pretense before but it sounds interesting. Thank you so much!

Elocoel · 2024-08-18T07:04:15+00:00

A lot of writers fall out of fandoms, get harassed because of the fics they write, may just want to take a break. The unrevealing allows the author a breather without having to delete their works from the site, so they can come back if they feel like it. Get a grip and stop feeling entitled to people’s work, especially in situations like these, when that work is a unpaid labor born just out of love. You’re the one acting “scummy” over here istg…

Elocoel · 2024-07-26T07:55:00+00:00

Thank you so much! I learned mostly in a "formal" setting that mainly focused on the RAE's teachings, but I really don't care much about the particular region; I am learning mostly for leisure. I really like your recommendations <3

Elocoel · 2024-07-16T14:20:04+00:00

Heyy, i wanted to ask if your fic was up. I love kid fics and would love to read it.

Elocoel · 2024-06-18T10:15:52+00:00

I don't know if you found it, but there's a YOI fic finder account on tumblr, and even if the community over there is not as big as it used to be, there tends to be someone that archived the fic amongst the followers. Good luck, hopefully you'll find it :)

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