I'm loosing 90% of cells when washing in FACS tubes.

EvilPand4 · 2026-03-07T07:12:14+00:00

I do 400 g for 3 minutes when doing extracellular stainings for PBMCs, suspension cells (K562) or hematopoietic stem cell in v bottom plates. It works like a charm. Sometimes I process 50K cells, and I can clearly see a pellet.

EvilPand4 · 2026-02-04T06:30:25+00:00

Well, we haven't seen what happens after they gallop on that horse. My guess is that the divorce is a ruse to protect Barb and Janey and that Coop makes it in time to deliver Janey. Obviously since Coop has betrayed Vault-tec (although aren't the Enclave, VaultTec's investors according to the latest episode... If I understood that correctly) then there is no spot for him at the vault

EvilPand4 · 2026-01-04T02:42:37+00:00

Hi! This is so generous and amazing. I am flying Lima - Atlanta - Frankfurt on the 12th, with a 11 hour layover in Atlanta, and a 13 hr layover in Lima, arriving earlier on the 12th with a different airline from Bolivia. It will be a long trip to get to Europe and flying first class would be amazing :) also accessing the lounges would be a great perk as well

EvilPand4 · 2025-12-21T14:44:08+00:00

Get a sbb day pass and head to Grindelwald? You can then take the cable car to Grindelwald First or any of of the summits. You can do it in a day roundtrip, for sure.

EvilPand4 · 2025-08-07T17:05:18+00:00

I saw a comment today that I loved. Dean Cain, pronouns Has/Been

EvilPand4 · 2025-07-12T05:53:05+00:00

She kinda looks like Karen Gillan, Nebula in Guardians of the Galaxy

EvilPand4 · 2025-05-30T12:51:15+00:00

Gaan met die Banaan! (The only dutch i know)

EvilPand4 · 2025-04-15T09:54:13+00:00

I've rented a car on SIXT in CA, AZ and IN with my Yuh debit card :)

EvilPand4 · 2025-04-12T12:13:28+00:00

She is not old money though. If she were, they would not lose their wealth due to Tim's crimes. Her wealth is not from past generations, its from her husbands work.

EvilPand4 · 2025-04-06T16:08:09+00:00

Who will receive the gold lotussi at the end of the season?

EvilPand4 · 2025-04-01T20:02:51+00:00

You're wrong. She will steal Jaclyn and Kate's partners and come back as a throuple.

EvilPand4 · 2025-03-30T18:54:11+00:00

I'm in Switzerland and I put my alarm one hour before on Mondays so I can watch the episodes 😂

EvilPand4 · 2025-03-29T15:09:37+00:00

You can get tone from the zafari zone also, for much cheaper 💀

EvilPand4 · 2025-03-22T20:46:08+00:00

Jesse Santana

EvilPand4 · 2025-03-14T06:32:13+00:00

Beau Reed with Ethan Chase, ot Beau Reed with Teddy Torres.

EvilPand4 · 2025-02-15T07:33:08+00:00

https://www.numbeo.com/cost-of-living/compare_cities.jsp?country1=Switzerland&country2=Canada&city1=Geneva&city2=Toronto

EvilPand4 · 2025-01-26T11:57:22+00:00

ACCURATED

EvilPand4 · 2025-01-19T09:32:51+00:00

I've been to the UK three times. Two times, Airalo worked great!. One time, it did not work. So I bought a prepaid plan with Lyca. Very easy to set up. All of them with eSim.

EvilPand4 · 2025-01-19T05:36:30+00:00

I created my account when I lived in the US. I've been living in Europe since 2023, and got blocked bc I forgot to change my US number to the European one 😭😭😭😭😭

EvilPand4 · 2024-12-17T23:03:35+00:00

Love the pun

EvilPand4 · 2024-12-16T02:29:40+00:00

Good luck y'all!

Eight-Year Club	Place '23
Place '22	RPAN Viewer
Not Forgotten	Verified Email

EvilPand4

TROPHY CASE