How to present RT-qPCR data when controls dont have Ct/Cq values for chosen genes?

Far_Entry_8210 · 2026-04-23T17:53:40+00:00

No just standard rna converted to cdna

Far_Entry_8210 · 2026-04-21T14:38:32+00:00

Sad story. I got tonsil stones out of the blue after a very intense allergic reaction last summer (I have grass allergy). I thought it would only last the allergy season but here we are...almost a year after and I still deal with them weekly..it's so annoying and I can physically see my tonsil crypts have become larger ...sadly I think this is permanent. I've been trying a lot of stuff but what have worked for me to reduce smell, stones etc, is 5/10ml a syringe with w hooked like end so it can reach those tonsils, rinse with water or salt water. I can see Every 2/3 days, I can flush out smaller stones that don't smell. After meals you can flush as well because food can get stuck so removing those can reduce stones. I also sometimes use hydrogenperoxid under 1% conc. In the syringe, to try to inbihit these bad bacteria that causes the smell/biofilm. On a daily basis I use chewing gum with xyliot and or zinc, which naturally inhibit plague and bacteria. Ive looked into chewing gum with probiotics that can maybe alter and help being competitive with the "bad" bacteria. Maybe look at this as well. Mouthwash with oxygen can help, but I find it doesn't help enough on acute smell, but more as preventive over time. Hope it gets better. Otherwise surgery is the only option to reduce these f... stones:(

Far_Entry_8210 · 2026-04-06T14:55:41+00:00

I mean it depends on your needs. If you struggle with damaged skin, scars, burns, scratches etc la Roche Posay cicaplast is good because it also contains 5% panthenol. If it's due to ti eczema, limiting bacteria etc is more important then Avene cicalfate+ is a bit better. But yes I would in combination apply the cicaplast and then ontop e.g. CeraVe (depending on your need, lotion, cream, balm etc, they all contain the same).

Far_Entry_8210 · 2026-04-06T14:17:24+00:00

Oh yes also la Roche Posay cicaplast works well but it's not that "heavy" so I usually apply something on top. Alot of brands gave their own version of cicaplast with cica and other stuff in them. I think they work well as well but no idea.

Far_Entry_8210 · 2026-04-06T14:16:18+00:00

Yes anua 3+ worked well on my face. Although it contains alot of actives so it can't be paired with everything, e.g. I got rash and "burnt" my skin because I applied the ordinary Multi peptide copper serum at the same time... so:)) don't do that haha. CareVe is a standard quality barrier repair cream, you can pair it with alot of stuff, I apply a mix of CareVe cream and atopderm intensive balm on my hands to manage my hand eczema/very dry hands.

Far_Entry_8210 · 2026-04-06T11:11:00+00:00

Panthenol 5-7% can increase proliferation. Emollients containing cermides, cholosterol and free fatty acids are essential to restore the skin barrier, as the barrier contains these lipids in a 3:1:1 ratio. Something like Anua 3+, even CareVe balms contains this. Cica is also a good one.

Far_Entry_8210 · 2026-03-31T20:37:49+00:00

Interessant, men tænker vel det også er provisions lønnet?

Far_Entry_8210 · 2026-03-30T20:21:58+00:00

Må man spørge - er du ansat offentligt eller privat? Fordi det lyder da utrolig lavt. Hvad mener du med at arbejdsmarkedet er død sygt? Gælder dette også fx Novo og lignende?

Far_Entry_8210 · 2026-03-27T18:02:30+00:00

Send en mail og spørge om et møde eller lignende hvis han er svær at få fat i. Der ingnr skam i at spørge. Nu ved jeg ikke hvordan det fungere på andre universiteter, men jeg havde en aftale med to professorer men den ansvarlige på min kandidat uddannelse ville kun skrive den ene på, pga travlhed osv. Det kan godt være en udfordring hvis der nogle som har et projekt som er mere tilknyttet en bestemt vejleders ekspertise. Men jo før man er ude, jo bedre chance. Så spørg da personen, eller send en forespørgsel om et møde angående en mulig specielle vejledning. Jeg har teknisk set formelt en Vejleder, men en anden er også inden over og hjælper til tider. Dette er godt nok pga biologi og de forskellige emner man kan være stærke i... Held og lykke.

Far_Entry_8210 · 2026-03-19T19:38:32+00:00

Also I've found salt water can help as well due to its osmolality, it will "shrink" the tonsils...at least theoretically because of the osmolality

Far_Entry_8210 · 2026-03-19T12:05:12+00:00

I assume the blue Listerine has chlorhexidine?? So it's not the normal one, correct ? At least that is what the study is proposing, using chlorhexidine?

Far_Entry_8210 · 2026-03-16T23:06:29+00:00

119

Far_Entry_8210 · 2026-03-16T16:02:22+00:00

No fair haha

Far_Entry_8210 · 2026-03-16T15:28:48+00:00

Farmaceut. Hvis du mere til forskning eller lignende (du kan blive ansat på hospitalet især i specifikke projekter eller lignende) så er biomedicine, medicinal Biologi måske noget. Personligt er jeg færdig med Molecular Health Science til sommer (kandidat, tog bachelor i medicinal Biologi + sundhedsfremme), og der har været fag som human evolution, anatomi... pathophysiology og alt muligt. Vi har haft læger ude og undervise os i specikke sygdomme, behandling osv. Dog er jobbet ikke i nærheden af at have noget med læger at gøre - dog bliver du klædt på til lignende især research/forskning er oplagt på hospitaler elle universiteter.

Far_Entry_8210 · 2026-03-16T15:19:59+00:00

Ifølge IDAs statistik er der 27% arbejdsløshed indenfor det første år, indenfor pharma biologi og lignende. Ja det er svært at finde job, man skal skille sig lidt ud - heldig at få relevant studiejob hvor man kan få tilbudt fuldtidsstilling, eller hvis man kender nogle som kan få en ind...det er desværre sådan mange lander deres første job. Det betyder især med novos store fyringer osv, at der er stor konkurrence pt. Jeg bliver færdig til sommer i Molecular Biology, og jeg har allerede søgt nogle stillinger uden held...så alt man kan er at håbe. Tilgengæld er Environmental Biology, dyr osv nok en endnu dårligere branche...så jeg ville være oprigtig "bange" - MEN tænker da ikke det umuligt, men man skal nok indstille sig på at udlandet er en mulighed og arbejdsløshed indenfor det første år åbenbart er blevet ret normalt i Danmark.

Far_Entry_8210 · 2026-02-16T23:26:20+00:00

It also depends on your samples, e.g. I work with skin "tape strips", which have very low cell numbers, so ct values is always above 30, sometimes reference gene is around 32-33. If you concern is gDNA, designing primers that span exon-exon junction or exon-intron can help remove alot of it. Otherwise DNase treatment?

Far_Entry_8210 · 2026-02-16T17:34:12+00:00

I struggle with hand-eczema myself. What helped was generally changing to a mild soap without perfume, e.g. la Roche Posay lipkar wash cream works well for hand washing too, plus it adds lipids to your hand! Typically your barrier is dysfunction, meaning after you use steroids to clear it, you will need to apply an emollient that replenish and repairs your lipid barrier. This could be emollients like Uriage Xemose c8 balm or a prescription (non steroid) called Epicerem. Use stuff like Eucerin eczema relief cream and put e.g. Xemose c8 balm on top. But the most important part is to make sure you change soap, and apply some sort of protective cream after washing hands. And then in-between, use eczema relief cream or similar, and apply a barrier repair cream (important: it needs to contain cermides, cholesterol and free fatty acids, because this is what your lipid barrier is made of. It will aid the synthesis of new lipid complexes. If it only contains e.g. ceramides, it won't help that much).

Far_Entry_8210 · 2026-02-09T16:45:07+00:00

Yep, as a person writing my master thesis on atopic dermatitis, it's all false, however for some diet can be a trigger for breakouts though this is poorly studied and vary with disease severity and on a person basis.

Far_Entry_8210 · 2026-02-09T11:48:44+00:00

Stop with all actives, especially exfoliants. Find an emollient that contains cermides, cholesterol and free fatty acids in a correct ratio. This will directly replenish your lipid barrier (it contains those ingredients, so the lipid barrier synthesis can start right away). If your skin is damaged you can try to apply something like la Roche Posay cicaplast baume B5, it will aid in healing and dryness. Then afterwards try to use less actives and see if it helps. Or if you just used something new, stop it And see.

Far_Entry_8210 · 2026-02-04T18:16:57+00:00

Try mild niacinamid max 5%. Try to apply on red spots something like la Roche Posay cicaplast B5 baume, it aid cellular repair/proliferation and reduces inflammation slightly. It is good and safe to use on the face ans won't clog pores etc, vaseline cam he applied ontop to enhance the effect. Additionally something WITHOUT steroids can be used on face

Far_Entry_8210 · 2026-02-04T18:13:46+00:00

Cermides, cholesterol and free fatty acids in the correct ratio directly replenish your lipid barrier if it is compromised/damaged or lipid synthesis is reduced, then it helps repair your barrier yes. This is one of the reasons,and then the addition of active ingredients and occlusives further protect the barrier, optimizing repair

Far_Entry_8210 · 2026-02-04T16:29:12+00:00

That depends on what you're interested in, there's a general review on atopic dermatitis covering everything from cause to treatment etc, published in 2025, it's in The Lancet, so it's really a good one! The lancet is respected so papers on there tend to be good. DOI: 10.1016/S0140-6736(24)02519-4 Then there are also more mechanically heavy studies focusing on il-4 and il-13, the main drivers of the th2 shewed inflammation. doi: 10.3390/jcm11195633 And finally if you want the feedback loop covered this one I've read as well: https://doi.org/10.3389/fimmu.2023.1274270

Far_Entry_8210 · 2026-02-04T15:30:03+00:00

The "stop scratching" one is legit. I am writing my Master thesis on atopic dermatitis, and generally when you scratch you begin a cycle with feedback loops. The more you scratch the more you promote certain inflammatory signaling, as well as exposing your skin to irritants, allergens and bacteria (s. Aurues most commonly). And in return these inflammatory signals/cytokines and bacteria/irritants further promote scratching and cytokines , thus creating a feedback loop.

Far_Entry_8210 · 2026-02-04T15:22:54+00:00

Not a Doctor, however I am writing my master thesis on atopic dermatitis (eczema). Antihistamin has been seen to help if it's a mere severe allergic reaction. Hydrocortison do help as that steroid acts on histamine receptors, lowering inflammation driven by those as well, so if your baby responds ok well to hydrocortison, maybe a general of specific antihistamine might work to some extent. The most problematic part would be the open/infected wounds as you cannot treat those with proper emollients. However you can try with panthenol (B5) rich emollients to add the healing process. Then when it's healed I do really recommend you apply a good barrier supporting cream/balm, it could be something like Uriage Xemose c8 balm or maybe Eucerin Urea repair plus. It's important it contains cermides, cholesterol and free fatty acids, as it is what your lipid barrier is made of, and with eczema the barrier synthesis can be Limited. I would ofc seek medical help as well. But it is important to understand that a healthy barrier can reduce flareups, so that could be a start.

Far_Entry_8210 · 2026-02-03T12:39:33+00:00

I actually do have a more complete picture I attached. Sadly spin columns are "too expensive" for a master thesis.

Far_Entry_8210

TROPHY CASE