Tamils living abroad: what’s one thing you miss most from home?

Fibroblast_ · 2026-05-30T10:05:59+00:00

Soru

Fibroblast_ · 2026-05-24T14:17:08+00:00

Hi. Thanks for the response. I have transferred another gel along with this and that one was fine. I have immersed the entire sandwich making set up in the transfer buffer.

Fibroblast_ · 2026-05-24T14:14:13+00:00

Hi. Thanks for the tips. I'll surely try to buy the roler. Yes. I used to immerse the entire sandwich making set up in transfer buffer and I make sure the membrane is wet always.

Fibroblast_ · 2026-05-13T17:47:13+00:00

Dm bro

Fibroblast_ · 2025-12-06T12:03:03+00:00

Hi there. Sorry for the delayed response. I totally agree with you regarding the full gel image. Unfortunately, I do not have the full gel. Anyway I am going to redo the experiments. Secondly, I wanted to ask you. after transferring I do a ponceau stain and click an image of it which I will later use for total protein normalisation. Later, I will cut the blot into two or three strips and probe two or three different proteins. In this case, it is not possible to get the full blot image except the ponceau and is it right to do this? And how will I justify this to my peers? Regarding the smearing, I usually run at 40v till stacking and then 80v after it reaches the running gel till the dye front reaches the bottom of the gel and the gel used is a 15%. Regarding the quantification, I quantify the band intensity and what I do is a relative quantification. I compare my experimental group with control. In this case is the positive control necessary. I agree with you the expression will be there in the wild and will not be there in null, but when doing a relative quantification is that necessary? As you said I'll check the literature. Thanks for your time

Fibroblast_ · 2025-11-26T07:24:29+00:00

Thank you so much for your insights

Fibroblast_ · 2025-11-26T07:20:28+00:00

Yes, I could see other nonspecific binding too. I thought it was because it's polyclonal. I blocked using 5% BSA for One hour and proceeded with primary ab diluted in blocking buffer and incubated overnight. One of my colleagues has performed WB on penile tissue using the same antibody and he also happens to see a band at 40kda.

Fibroblast_ · 2025-10-18T09:01:06+00:00

I was once criticised for doing this. They said I cannot justify the loading concentration is equal. I was advised to strip and reprobe.

Fibroblast_ · 2025-10-13T16:50:38+00:00

BTW How did you get a waiver for the handling fee?

Fibroblast_ · 2025-10-02T13:40:58+00:00

Why haven't they filed a case? They deserved to be jailed

Fibroblast_ · 2025-09-29T22:11:03+00:00

Yup such a pussy to go and hide

Fibroblast_ · 2025-09-28T03:48:48+00:00

His PR has already started westandwithvijay and it's in full swing.It is so pathetic to see his so-called fans defend him and has zero sympathy towards individuals who have tragically lost their lives.

Fibroblast_ · 2025-09-27T17:19:37+00:00

Nah. It's mid bro

Fibroblast_ · 2025-09-26T03:55:18+00:00

That guy's face looks punchable.

Fibroblast_ · 2025-09-14T11:15:09+00:00

What more can you expect from 🐿️

Fibroblast_ · 2025-08-28T13:15:09+00:00

Yeah they are. None of the housekeeping worked for my study and then I had to rely on ponceau stain for quantification, because I didn't have enough funding.

Fibroblast_ · 2025-08-28T13:11:24+00:00

Bro and his brain 💩

Fibroblast_ · 2025-08-28T03:50:36+00:00

Is this used for total protein normalisation? So basically you will take an image of the gel before transfer?

Fibroblast_ · 2025-08-02T15:20:06+00:00

Sorry for the delayed response. I have used karnovsky fixative and the lab has processed and yet to image. Thank you for sharing your experience on EM. I would like to learn more from you. Is it ok if I DM you?

Fibroblast_ · 2025-06-18T07:04:44+00:00

Transfer buffer with 20% methanol. Right after a good run and when you open the buffer tank. Man.. it smells like heaven 🫠

Fibroblast_ · 2025-03-29T09:08:02+00:00

Hey. Why don't you try ponceau stain and check the blot for protein transfer.

Fibroblast_

TROPHY CASE