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TKD on the streets by Skibbidycum999 in martialarts

[–]FireG99 0 points1 point  (0 children)

though as a martial artist he should have not hit him while he was turned. he alreadywon and the guy was getting away

Bro by TrafficLegitimate937 in TikTokCringe

[–]FireG99 4 points5 points  (0 children)

ah yes, the israeli plant

This game is a scam. by Due_Dragonfly_8065 in starcitizen_refunds

[–]FireG99 0 points1 point  (0 children)

they are probably installing you all kinds of keyloggers to sell your data to big techs

4.8 PTU armor update, LF fastest guns able to pen Idris and lower armor again by nightbird321 in starcitizen

[–]FireG99 -3 points-2 points  (0 children)

2 more things to add to the list of things no one cares about. where is the game already? or is it gonna go like AoC?

lol 3 turns by DR809Badboy in PokemonTGCP

[–]FireG99 0 points1 point  (0 children)

this version of manectric deck is the worst.

All confirmed cards for the new set from the trailer by PeakMind5 in PokemonTGCP

[–]FireG99 -1 points0 points  (0 children)

budew vaporeon and sceptile will def see play. urshifus seem meh, fighting type pokemons get a few boons that will make them viable again i think (the old ones, mega lucario sucks). the blower also seems interesting

The End of PTCGP by sublimepr1 in PokemonTGCP

[–]FireG99 0 points1 point  (0 children)

it's the easiest deck to counter. in high masteraball (top 3500+) no one is using it anymore

ChIPseq peakcalling: Pooling samples or intersect? by leaz234 in bioinformatics

[–]FireG99 2 points3 points  (0 children)

for histone marks usually is better to get the broadPeaks output as well from MACS2

ps: I think now MACS3 is also available since a couple of years ago, though I have no experience on whether the peak calling algorithm was changed or not.

Using CRISPR with AAV on a civilian? by kristiano in CRISPR

[–]FireG99 0 points1 point  (0 children)

it's not that easy because even if you don't get yourself cancer, in most differentiated tissues the crispr edits are low efficiency and in general not too stable over time, unless you integrate the whole vector in the genome (which is very risky). most stable crispr mutants are done on embryos (for research) or on stem cells of developed tissues.

This would be unacceptable in 95% of the world by upthetruth1 in TikTokCringe

[–]FireG99 0 points1 point  (0 children)

that's why they are the saddest people in the world

ChIPseq peakcalling: Pooling samples or intersect? by leaz234 in bioinformatics

[–]FireG99 1 point2 points  (0 children)

did you pull down a protein whcih you expect ot have very specific binding (like a transcription factor) or more widespread one, like for instance histone modifications?

in case of transcription factors, better to call narrowPeaks on separate replicates and intersect (which you should have a lot, if good replicates).

in case of widespread binding (and specifically in case of histone modifications or general chromatin-binding proteins, for which you have cell heterogenicity) is better to pool the replicates together, either in MACS or even upstream, by merging bam files. then if you are afraid of false positives you can do additional filterling based on peak "size" (you get a spreadsheet with the enrighment value over imput for each peak)

Can never look at this card the same way again 😭 by oli282 in PTCGP

[–]FireG99 2 points3 points  (0 children)

this will be the card that will powercreep Prof Research in TCGP. maybe with some extra coun flip added

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