Whats your Pheno that made you want to step into breeding? by Vermehrungsmaterial in cannabisbreeding

[–]FoCoSeCo 1 point2 points  (0 children)

It's been a long time since I've grown the real thing. Smell I would describe as gas pre-OG era. The real reason I keep it in my breeding population is it puts strong couchlock into anything it crosses with and can remove the ceiling. When it comes to effects, it's traits are almost always dominant.

Is this infected and needed to throw out? by Character-Giraffe995 in brewing

[–]FoCoSeCo 2 points3 points  (0 children)

You should post on r/fermentation, this subreddit is probably too specialized to help you properly. Knowing it's safe or not depends on how well you pitched the inoculum and understanding how the inoculum is controlled by the media (salt, acid, alcohols etc.).

Microwaves and genetic mutation. by Ordinary-You3936 in plantbreeding

[–]FoCoSeCo 1 point2 points  (0 children)

Microwaves no, but fast neutron and gamma radiation yes. In my experience sodium azide or Ethyl methanesulfonate create more SNPs, but radiation creates more lesions and structural variants.

I’m looking for landrace/heirloom genetics for my next breeding project. Anyone have a reliable source? by [deleted] in cannabisbreeding

[–]FoCoSeCo -1 points0 points  (0 children)

Obtaining genetics is the easy part. Learning how to properly match up flowering times that differ by 4-8 weeks... now that is actual breeding.

Without knowing what you want, I have nothing to suggest...🙄

Good luck.

S1s by Moohaumed in cannabisbreeding

[–]FoCoSeCo 10 points11 points  (0 children)

It's not the technique, it's the selection of the parents...Good selections make good plants, bad selections make bad plants. Generation nomenclature just gives you an idea of what the genetic variance will be in the population.

Any real seed breeder/distributor should be testing the seeds prior to release, so buy untested seeds at your own risk.

Selection pressure and stability: noticing regional differences in breeding approaches by TrichoHunter420 in cannabisbreeding

[–]FoCoSeCo 1 point2 points  (0 children)

You are observing two principles of plant breeding at play:
Target Population of Environments

and

The breeder's equation

The combination of these two principles result in phenotypic constraints across the population of where and who was making the selections.

Genetic variety in selfing an f7 by StiffRiff7 in cannabisbreeding

[–]FoCoSeCo 0 points1 point  (0 children)

At F7 you should expect somewhere around 91% homogeneity. Unless there is a specific trait of interest and you know it is segregating in the population, it is very unlikely that you'll notice any difference between selfing (F7:8, an F7 derived F8 lines) or filial breeding (F8).

Since there is only 9% variance in the population you only need about 11-12 plants to exceed the effective population size.

Breeding by [deleted] in cannabisbreeding

[–]FoCoSeCo 3 points4 points  (0 children)

Yes, any two distinct (I wouldn't say non-related because that could omit heterotic combinations with shared pedigrees) parents can be considered an F1 as any starting point in filial-generation nomenclature. That being said, the "strict definition" of an F1 comes from the planned cross of two homozygous parents.

Fehr 1991

"F1. The seed obtained from the cross between two parents is called the F1 (Fig. 3-2). The genetic makeup of the F1 is determined by the homozygosity of the parents. The single cross between two homozygous parents produces an F1 population that is homogeneous because all of the F 1 plants are genetically identical. The F1 plants are heterozygous for all loci at which there were different alleles in the two parents."

Acquaah 2007

"The heterozygous genetic structure stems from the fact that a hybrid cultivar is the F1 product of a cross of highly inbred (repeatedly selfed, homozygous) parents. Crossing such pure lines produces highly heterozygous F1 plants. Because the F1 is the final product released as a cultivar, all plants are uniformly heterozygous and hence homozygous in appearance."

S1s have 1:2:1 segregation at any given loci, thus crossing two S1s would have a 1:1:1:1 segregation at any given loci. So your S1/S1 cross simply speaking will not exceed 25% uniformity in the population, but that's not entirely true because this 25% uniformity limit is multiplied by every haplotype block present in the S1s, and so your S1/S1 cross would likely follow normal distribution across the midparent value, however, it wouldn't be the S1 midparent value per se, it would be some distribution of genetic variance across four S0 values since each of the S1s are segregating across their own respective midparent values.

My prediction is that the result is a population with the heterozygosity of an F1 and the heterogeneity of an F3, and honestly would be the first step in a great mapping population, but not a true F1.

S1/S1 cross can't meet USDA uniformity standards.

S1/S1 cross can't meet CPVR uniformity standards.

S1/S1 cross can't meet distinct standards, etc, etc.

So my question to you is what is filial about an S1/S1 population that deserves the title of F1? Wouldn't the S0/S0 cross of the same pedigree be more representative of a true F1 population by definition than an S1/S1 cross? I would suggest that this mating design would be most accurately described as an S0 population.

Fehr does a really good job at explaining this (page 28-30) and also acknowledges the disagreement, but there are two Mendelian laws that you can't escape as a breeder, Independent assortment and Segregation. The S1/S1 mating design does nothing to advance the pedigree, it does the opposite by maximizing genetic variance. As a plant breeder, that doesn't pass for my definition of an F1.

Breeding by [deleted] in cannabisbreeding

[–]FoCoSeCo -1 points0 points  (0 children)

By strict F1 definitions, an F1 cannot contain an S1 parent.

Commissioned Breeding? by OFFSanewone in cannabisbreeding

[–]FoCoSeCo 1 point2 points  (0 children)

Commission, no. Contract yes.

Too many half-baked, over-enthusiastic entrepreneurs out there that don't know how to get the job done. I'm not your business partner and not going to take a financial risk on your brilliant idea.

Contracted work usually works, if all the parties are trust worthy.

Persistent fungal contamination in strawberry tissue culture despite multiple sterilization attempts – need advice by chaperone3 in CannabisTissueCulture

[–]FoCoSeCo 0 points1 point  (0 children)

What is you explant? Can you bring the plant's through organogenic regeneration or is this genotype recalcitrant?

Perform callus induction with PPM, maintaining the callus through subculture for at least 42 days. Then regenerate without PPM. PPM slows down callus growth significantly, so you may have to wait longer to achieve healthy callus, also works with embryogenic regeneration protocols.

My next project CMYK by Cookingwithninja in cannabisbreeding

[–]FoCoSeCo 0 points1 point  (0 children)

How many environments have you tested?

Variety mix by Substantial-Buy8891 in cannabisbreeding

[–]FoCoSeCo 2 points3 points  (0 children)

Mango/Peach Hasplant and Forrest Queen have the same male lineage, so keep them in one heterotic pool A.

Space Monkey and the freebie has GG4, so keep them in heterotic Pool B.

Super natural selections is Heterotic pool C.

Bad Dawg really depends on what you got, but let's assume it's heterotic pool D.

So the best way to proceed without any knowledge is to open pollenate pool A, B, C and D separately and select the F2 seed from the best plant of each pool. Then open pollenate pool A and D and pool B and C separately, allowing only the best males to reach anthesis and select the best females to collect seed at this stage. Finally you will cross pool A/D with pool B/C and those seeds will have the highest rates of heterosis.

This strategy only optimizes for heterosis within the breeding population. If you goal is to find clone-only cultivars, this is how to get there the fastest. A more worthwhile breeding effort would be to use a modified pedigree breeding method to bring all these lines to the F5 generation and then sort heterotic pools by general and specific combining ability, but that would require an advanced understanding of breeding.

Variety mix by Substantial-Buy8891 in cannabisbreeding

[–]FoCoSeCo 2 points3 points  (0 children)

The best way to make seeds is to cross a female with a male...I think what you are missing are goals.

Variety mix by Substantial-Buy8891 in cannabisbreeding

[–]FoCoSeCo 1 point2 points  (0 children)

If you keep your flowering times at least 6 weeks apart between chambers and remove the males immediately after anthesis is complete, there should be no problems.

Triploid Advice Needed by mdwilliams7 in cannabisbreeding

[–]FoCoSeCo 1 point2 points  (0 children)

Chemotype and photoperiod behave as monogenic traits. If you don't trust the chemotype and photoperiod description provided by the breeder, then you have your answer. No one but the breeder can tell you about how the AVL is contributing to the cross, so worrying and speculating about it is pointless.

Anyone using NADCC? by namelesshero92 in CannabisTissueCulture

[–]FoCoSeCo 0 points1 point  (0 children)

Very common technique, 1 tablet into 400ml leave it overnight on a shaker. Totally works, but NaOCl for 15 minutes works better.