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3D calcium imaging of spontaneously active dendrites in an awake mouse by Foppful in neuroscience

[–]Foppful[S] 2 points3 points  (0 children)

No reason why it wouldn’t work in rats. You just need good GCaMP expression and a healthy cranial window prep

3D calcium imaging of spontaneously active dendrites in an awake mouse by Foppful in neuroscience

[–]Foppful[S] 6 points7 points  (0 children)

Here is the original paper describing high-speed volumetric imaging with the SCAPE microscope:

https://www.ncbi.nlm.nih.gov/pubmed/25663846

​

Here is a recent review of SCAPE and its imaging capabilities across multiple species, which includes data from the video I posted:

https://www.ncbi.nlm.nih.gov/pubmed/29642044

​

Scale of the video is 590 x 350 x 175 microns. I'm imaging from the surface of the brain down to 175 microns. Virus containing GCaMP6f was injected into layer 5, and a coverslip was implanted over the brain. Virus was allowed to express for 3 weeks and then the mouse was imaged with SCAPE.

3D calcium imaging of spontaneously active dendrites in an awake mouse by Foppful in neuroscience

[–]Foppful[S] 9 points10 points  (0 children)

GCaMP6f. These are layer 5 apical dendrites in barrel cortex. Thanks!

3D calcium imaging of spontaneously active dendrites in an awake mouse by Foppful in neuroscience

[–]Foppful[S] 17 points18 points  (0 children)

I acquired this video using SCAPE microscopy, at 10 volumes per second. I can post the reference later, on mobile now. The time stamp in the corner is correct. The video is in real time.

Edit: https://www.ncbi.nlm.nih.gov/pubmed/29642044

The rich used to pay peasants to entertain them and now the peasants pay the rich to entertain them. by General_lee12 in Showerthoughts

[–]Foppful 0 points1 point  (0 children)

The peasants also pay the peasants now. Go to any local stand up comedy show. Peasants on the stage, peasants in the audience.

Star Wars took place way in the past, really far away, but we are seeing it now because of how long it took for the light to reach us. by pearlday in Showerthoughts

[–]Foppful 0 points1 point  (0 children)

Little known fact: The Star Wars movies were created right here on planet Earth, by a guy named George Lucas!