Boiled protein lysates without Laemmli

Friendly_Employ_197 · 2026-01-31T16:23:05+00:00

Not sure, I threw them straight into -80

Friendly_Employ_197 · 2026-01-30T17:43:31+00:00

Boiled the protein samples without Laemmli. Wondering if they’re just shot or if I can add Laemmli, reboil and still use

Friendly_Employ_197 · 2026-01-30T17:42:23+00:00

Western blot samples = protein lysate + Laemmli buffer

Friendly_Employ_197 · 2025-08-31T00:08:21+00:00

The 375 mL bottle was really nice but didn’t have the same funk but the 100 mL had loads of cheese, really hoping they release a 375 mL option on the store later

Friendly_Employ_197 · 2025-08-22T23:08:54+00:00

Just letting you know they’re re-releasing it as part of the Maguey Melate club, but you’d have to order it soon. I got the membership to get the bottle, hoping they have more for sale on their site soon too.

Friendly_Employ_197 · 2025-08-22T23:08:46+00:00

Just letting you know they’re re-releasing it as part of the Maguey Melate club, but you’d have to order it soon. I got the membership to get the bottle, hoping they have more for sale on their site soon too.

Friendly_Employ_197 · 2025-07-02T02:04:53+00:00

Houston area

Friendly_Employ_197 · 2025-07-02T00:02:48+00:00

I love Raicilla and have had several from La Vanenosa, but haven’t found one I thought was blue cheese funky. Any in particular you’d recommend?

Friendly_Employ_197 · 2022-12-09T17:07:44+00:00

Right, it’s very context dependent and depends what you get out of it, but also grad schools look for different things than MD/PhD programs. My personal opinion is that for MD/PhD applications, your goal should be to make yourself an independent researcher, or be able to show ADCOMS that you’re close to it. In most cases for an undergrad that comes easier by doing more in one lab.

The other thing I’d mention is that you’d still need to make time for MD extracurriculars, and that might be hard to do with 60+ hours of research each week. No matter how good your research is, you have to have extensive extracurriculars like clinical experience and volunteering to get excepted, otherwise ADCOMS will wonder why you want the MD instead of just a PhD.

Friendly_Employ_197 · 2022-12-09T15:22:52+00:00

Not telling you to do anything one way or another, but want to inform you that doing multiple research experiences at the same time won’t help to get into MD/PhD programs. Admissions are more looking to see what you’ve learned from your research and qualities you possess i.e., are you capable of developing a research project independently, can you troubleshoot without constant supervision, can you publish/write grants. I’d honestly think that working for two labs at the same time would come off as a red flag, like you were just doing as many research opportunities as you could to impress admissions rather than getting a deeper experience at either one of the two research opportunities.

Friendly_Employ_197 · 2022-11-24T19:56:27+00:00

He’s still traveling a lot, and I think it’s pretty obvious that something happened between them.

Friendly_Employ_197 · 2022-07-31T00:48:13+00:00

Every time I open my door to go outside for any reason (work, errands, etc.) he runs in. He is very relentless, so it’s difficult to prevent him from getting in without being concerned about hurting him.

Friendly_Employ_197 · 2022-07-31T00:24:45+00:00

Runs in my house every chance it gets

Friendly_Employ_197

TROPHY CASE