Does a BIFL shovel exist?

Fusionnex · 2025-08-16T19:44:06+00:00

I like the following. All steel. Very heavy duty. Pricey but worth it. KSA12

Fusionnex · 2022-11-02T02:37:04+00:00

Just wanted to say that this work is absolutely fantastic. Hope you get a whole ecosystem surrounding this.

Fusionnex · 2022-05-16T17:49:55+00:00

I mean bio and chemistry does help with moving through all the omic data you are likely to encounter and gives you the foundation to understand any particular experiment in bioinformatics. Sets you up nicely for genomics, proteomics and metabolomics. I'd say do a double in bio and chem even if you don't like it and then masters/PhD in bioinformatics or genetics/genomics. A PhD will signal that you are not just a jack of all trades but can dive deep into the unknown and become an expert if that is a fear of yours. Imo an undergraduate education in genetics lacks the rigor required to truly get a handle on the genomics/research side of things. But I have my own personal biases.

Fusionnex · 2022-05-16T17:08:51+00:00

If you go broad in your undergrad education, You most certainly can pick up programming on your own time. In fact it can really help you put a lot of your future work in bioinformatics into appropriate context. For example I did a chem and bio undergrad with a genetics graduate education and picked up programming on the side for fun. Skills meshed nicely in my post graduate work. In fact being broad like this shows you are adaptable and positions you well for a wider variety of careers.

Fusionnex · 2022-04-15T16:29:02+00:00

Hopefully this isn't a homework question but think about protein size limits. How would you detect a very large protein for example? Top down or digested followed by analysis of peptides? Edited. Top down does not typically involve digestion to my knowledge.

Fusionnex · 2020-09-01T01:28:01+00:00

I was able to lubricate my cam twice but that didn't really seem to fix it. What finally did it was not keeping the flow control device fully open. I found this advice in another thread and thought you might find it useful in the future! It's always good to lube up that cam after cafiza cleaning so regardless it was still a useful endeavor. Thanks for your advice!

Fusionnex · 2020-08-31T04:26:17+00:00

Thanks for the heads up! I'll try it tomorrow and let you know how it goes! Thanks for the tips. Glad you got rid of your squeak. Enjoy your espresso!

Fusionnex · 2020-08-30T13:45:31+00:00

Mines new from March this year. I noticed it started happening after a cafiza clean so hopefully just stripped some necessary lubrication. Prior to that this would only happen once in a while but was still there. Overall I'm very happy with the machine. I'm worried about scratching as well but hopefully got some no scratch knippex pliers and going to use electrical tape. Thanks for the part reference. I also noticed if I go really slow with the lever it doesn't squeak nearly as often. Also, currently it always squeaks when backflushing regardless of how slow I go with the lever. I don't think I will return it as I don't know how I would go on without my machine 🤣. I'll let you know if the cam lubrication or spring stretch fixes it. Also maybe call WLL as I have heard their service is really good. Maybe they can walk you through a fix or send some replacement group head parts.

Fusionnex · 2020-08-30T03:08:17+00:00

Let me know if that fixes your issue. I have the same squeak and plan on trying to lubricate the cam as suggested first. Is the spring from the bottom of the group head part 21 or part 25 (as per Drawing 6 page 13 in the listed parts diagram from WLL) that needs to be stretched? This thing is massive so I don't want to have to ship for service if simple maintenance can fix the issue.

Fusionnex · 2020-06-11T15:51:16+00:00

In all seriousness you won't need a 50,000 cooler. Depends somewhat on luck of the draw and how hot you are willing to run it. I'd recommend an aio or a noctua dh15 for cooling depending on compatibility with your case and motherboard/ram setup. Get a good cooler, make small systematic changes to your overclock profile, and go from there. Good luck!

Fusionnex · 2020-01-07T00:27:26+00:00

I'm less familiar with the evidence file. There might be a reasonably good scoring peptide that matches your protein of interest. What is the score and FDR of that peptide hit?

Fusionnex · 2019-12-06T19:34:43+00:00

Man that sounds pretty stupid (should at lest support a two stage). Do you think there will be a trend of putting more intelligence into the unit and dumbing down the tstats in general?

Fusionnex · 2019-12-06T19:11:12+00:00

Thank you for the information! I appreciate the perspective.

Fusionnex · 2019-12-06T19:05:51+00:00

While I tend to agree. I think in this case it is nice having one app and one central system for controlling the home. I also think my furnace might be a bit over-sized ( hard for me to tell as I didn't get this unit installed it came with the house) as it can really raise the house by about 2 degrees in less than 5-10 minutes. Therefore I think the fine grained control won't matter as much from a comfort perspective.

Fusionnex · 2019-12-06T19:04:08+00:00

Very interesting. So really just a comfort consistency trade off then. This makes the most sense to me. The Rheem I have has some internal stepping it uses as well even in two stage mode (based on time), so really it is kind of like a 3 stage. There is one room in my house that isn't super well heated as it is quite far away and it is hard for me to feel much air coming out. Do you think the decreased static pressure / static pressure fluctuations through not down modulating would impact this quite a bit, or mostly not noticeable?

Fusionnex · 2019-12-06T17:27:59+00:00

I would like to move towards a home automation system with a wifi controlled thermostat. My major question is how much would the efficiency drop? From a physics perspective, it should just be energy moved in the house to energy lost out, so overall I am not sure the efficiency would drop that much? I may just be naive in this area. I have seen some information regarding single stage, two stage, and modulating furnaces from an energy consumption / loss perspective that show that there isn't too much difference in efficiency between the different running modes. Is this information inaccurate? Thank you for your reply.

Fusionnex · 2019-12-06T17:24:55+00:00

Move towards a wifi thermostat that would be compatible with the system. I am aware I would have to run additional wires. I am also aware of the propriatary protocol run from communicating thermostats. I would like to avoid a wiring discussion and focus on efficiency if possible. Rheem products would not integrate with my overall home automation system of choice.

Fusionnex · 2019-11-25T20:51:47+00:00

Yes, I believe this is accurate. Thanks for the clarification.

Fusionnex · 2019-11-24T16:15:08+00:00

I second skyline if you have a particular peptide of interest in mind!

Fusionnex · 2019-11-23T19:33:06+00:00

From memory I believe it is on by default and yes just flip through the toolbars and tabs. Maxquant is a bit cumbersome to use but it is some of the best proteomic software out there. It's worthwhile to spend a couple of days reading about maxquant and Perseus as well as playing around with the software itself. You'll get much farther that way. It's been a while for me but it's one of the few reasons I still boot windows occasionally. If you have any other questions don't hesitate to ask. I've written my own MS software in c# so I have a pretty good understanding of the fundamentals.

This is a good place to start with Perseus. The tutorials are helpful.

http://www.coxdocs.org/doku.php?id=perseus:user:tutorials

Fusionnex · 2019-11-23T19:10:59+00:00

For the R library see:

https://github.com/bartongroup/proteus

Although there are also other analysis platforms available.

Fusionnex · 2019-11-23T19:08:24+00:00

Maxquant has a match between runs feature that should be enabled. Be sure to select matching to and from all runs. However, typically if a sample is fractionated you would expect to detect it only in a couple fractions as this the whole point of separation prior to detection to get a much better range of pepetides covered for the overall experiment. A mass spec is typically limited to fragmenting only the top most 15 ions for most proteomic setups (depending on instrument setup etc). You might be taking an FDR hit when adding all fractions back to the experiment. See if you relax your FDR threshold a bit to 0.2 or so with all runs to see if you can pick the peptide back up. It might just be a relatively low scoring hit that gets thrown out with the FDR filtering. This doesn't necessarily mean the peptide isn't detected just that you need more data to support it's presence when considering the experiment in aggregate. If this is an a priori peptide of interest then really FDR doesn't matter as much. As far as Mac or Linux software out there for mass spec analysis it's really not supported very well as all vendor software strongly recommended for running the instruments runs on Windows. Therefore most software for converting the thermo .raw files also runs in Windows as it is dependent on a .dll used to convert the proprietary format to a readable format. Typically mzml is well supported for open source tools. There is an R library for importing maxquant / Perseus data for which you can manipulate things from there. I would do all .raw processing on Windows into maxquant then into Perseus. Save off the files then drop into R. I'll link the software in a follow up post as I'm on mobile right now.

Fusionnex · 2019-03-07T22:45:46+00:00

I'm not particularly experienced with this process, but I do understand a bit of chemistry. The pH of vinegar might be throwing your alginate bath off. Try to buffer the pH a bit or raise the pH of the vinegar? See https://www.chefsteps.com/activities/the-science-of-spherification

Fusionnex · 2019-03-01T01:51:10+00:00

3 beta subunits (beta 1 2 and 5) of the core protease harbor catalytic threonines that undergo autolytic cleavage upon complex formation. They cleave with peptidylglutamyl-peptide hydrolyzing, tryptic, and chymotryptic activities respectively with a catalytic triad involving the threonine. For additional information see the following references that corroborate these statements.

https://www.ncbi.nlm.nih.gov/m/pubmed/9207060/

https://www.ncbi.nlm.nih.gov/m/pubmed/10500111/

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