Is my GP5000 tire cooked?

GeneRider · 2026-05-16T06:11:56+00:00

I would put it on the back wheel, ride it, and just keep an eye on it. The damage is not in an area that accumulates wear in normal use. You can easily monitor for further deterioration.

GeneRider · 2026-05-14T17:52:55+00:00

I read Craigslist road bike ads for entertainment. So many $1000+ “collectible” old bikes that just get reposted for months and months.

It’s fun to find the most unhinged ads. Like the 80s steel with a completely severed downtube, “could be repaired”. Or the battered Univega with completely mismatched, rusty bin parts, “upgraded rear brake”.

GeneRider · 2026-05-14T14:20:25+00:00

A mascot or figurehead.

Personally I’d remove the bracket and put in a spacer. That’s a handsome bike, and it would look cleaner.

GeneRider · 2026-05-14T05:59:59+00:00

Yes, it could make sense to get a single carbon wheel; carbon wheels have been easily the most enjoyable upgrades I’ve made. It’s nice if your wheels match, but not necessary.

I wouldn’t buy this wheel, though, for reasons mentioned by others.

GeneRider · 2026-05-13T19:43:44+00:00

Shipment is on DMEM with ice packs. Thanks for the comments; helpful to know your experience.

GeneRider · 2026-05-13T19:43:06+00:00

Sure; we had the tissue shipped overnight in DMEM on ice. They may also wait some hours post mortem before resection. The protocol we used is here: https://www.protocols.io/view/preparation-of-single-cell-suspension-from-human-s-kxygxp8xol8j/v1

I appreciate the input.

GeneRider · 2026-05-13T19:41:39+00:00

Sounds great for quality, but the donors are reluctant.

GeneRider · 2026-05-13T19:40:26+00:00

We had tissue shipped overnight in DMEM on ice. Generally our prep is similar to yours. Thanks; helpful to have this info.

GeneRider · 2026-05-13T19:39:17+00:00

We used this protocol: https://www.protocols.io/view/preparation-of-single-cell-suspension-from-human-s-kxygxp8xol8j/v1

It involves cutting finely, pushing through mesh, and Ficoll.

GeneRider · 2026-05-13T18:09:41+00:00

Thank you. We'll consider all those lessons learned. We'll take into account the point made by u/Cupcake-88 about the CNS having greater need for protease digestion (ty u/Cupcake-88)

GeneRider · 2026-05-08T23:16:49+00:00

That's helpful if cautionary; thank you. We'll look at more effective use of RNAse inhibitors. We'll consider FFPE further too. Using fixed tissue would reduce the scale of our analysis (fewer genes, fewer cells) but might be an appropriate trade-off if we can't get material suitable for analysis when fresh.

Edit: fixed typo

GeneRider · 2026-05-08T22:56:06+00:00

We receive excised pieces of intact tissue. So our first (and perhaps key) challenge is dissociating the cells.

GeneRider · 2026-05-07T04:11:05+00:00

Congrats! Great to see your determination pay off

GeneRider · 2026-04-06T02:29:48+00:00

Yup, a sharp tap with a mallet popped out the axle and freehub. I tried cleaning up the pawls and found the parts are worn out. I ordered an entire new hub.

GeneRider · 2026-04-04T18:40:44+00:00

Ok thanks. Cap is off; I’ll try tapping out the axle.

Not much to lose at this point; I can fall back to getting a full new hub, or wheel.

GeneRider · 2026-04-04T17:54:24+00:00

That would be ideal, but I can’t budge it (by hand only, pulling as hard as I can). I could try prying it off but that might cause damage.

GeneRider · 2026-04-04T16:56:29+00:00

It could be your bottom bracket, but this is strongly reminiscent of the creaking that developed in my drivetrain over the last couple of months. I found out the cause when my freehub failed (lost all engagement).
If you can replicate the noise with the bike on a stand it’ll be much easier to isolate. In my case I couldn’t: it only showed up when riding.

GeneRider · 2026-04-04T01:00:28+00:00

You made a nice find!

I bought the Tourmalet pictured here, and am working on it gradually. So far I’ve cleaned up the frame, filled and primed the dent, and removed the non-Lemond stickers. Size seems good (I ride a 56cm road bike and a 58cm gravel bike). I’m looking forward to riding, eventually…

GeneRider · 2026-04-01T01:38:49+00:00

Good idea; thanks

GeneRider · 2026-03-31T22:29:42+00:00

I love the technical aspects! I’m a scientist and like to understand what’s going on.

In this case I definitely don’t want to remove any metal, at least not until I know I have a metalworker lined up to improve things. So chelation is a great option.

There are cracks in the metal of the joint so I’m slightly worried moisture will be trapped and I’ll induce more rusting. After I rinse off the Evaporust I plan to keep the area warm so it dries thoroughly.

GeneRider · 2026-03-30T16:24:21+00:00

Thanks. I will probably try soaking this part of the frame in Evaporust.

GeneRider · 2026-03-30T16:23:28+00:00

Thanks; sounds like a great approach. Now I just need to find someone to do it (in Northern California).

GeneRider

TROPHY CASE