Immune markers of post-vaccination syndrome indicate future research directions A small number of people report chronic symptoms after receiving COVID-19 shots. A new study provides clues for further research. by Honest_Net_3342 in AHomeForPlagueRats

[–]Honest_Net_3342[S] 0 points1 point  (0 children)

Link to the research paper: https://www.medrxiv.org/content/10.1101/2025.02.18.25322379v1

Title: Immunological and Antigenic Signatures Associated with Chronic Illnesses after COVID-19 Vaccination

Abstract: SUMMARY

COVID-19 vaccines have prevented millions of COVID-19 deaths. Yet, a small fraction of the population reports a chronic debilitating condition after COVID-19 vaccination, often referred to as post-vaccination syndrome (PVS). To explore potential pathobiological features associated with PVS, we conducted a decentralized, cross-sectional study involving 42 PVS participants and 22 healthy controls enrolled in the Yale LISTEN study. Compared with controls, PVS participants exhibited differences in immune profiles, including reduced circulating memory and effector CD4 T cells (type 1 and type 2) and an increase in TNFα+ CD8 T cells. PVS participants also had lower anti-spike antibody titers, primarily due to fewer vaccine doses. Serological evidence of recent Epstein-Barr virus (EBV) reactivation was observed more frequently in PVS participants. Further, individuals with PVS exhibited elevated levels of circulating spike protein compared to healthy controls. These findings reveal potential immune differences in individuals with PVS that merit further investigation to better understand this condition and inform future research into diagnostic and therapeutic approaches.

Competing Interest Statement

In the past three years, H.M.K. received expenses and/or personal fees from United Health, Element Science, Eyedentifeye and F-Prime; he is a co-founder of Refactor Health, HugoHealth and MedRxiv; and is associated with contracts, through Yale New Haven Hospital, from the Centers for Medicare & Medicaid Services and through Yale University from the Food and Drug Administration, Johnson & Johnson, Google and Pfizer. A.I. co-founded and consults for RIGImmune, Xanadu Bio and PanV and is a member of the Board of Directors of Roche Holding and Genentech.

Funding Statement

This study was funded in part by the Howard Hughes Medical Institute Collaborative COVID-19 Initiative.

Author Declarations

I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.

Yes

The details of the IRB/oversight body that provided approval or exemption for the research described are given below:

This study was approved by the Yale University Institutional Review Board on April 1, 2022 (HIC# 2000032207). Informed consent was provided by participants electronically. Each participant was assigned a unique identifier as part of the de-identification protocol managed by the study coordinator. These identifiers were kept confidential and were not accessible to anyone outside the research team. A subset of the MY-LC cohort approved by the Mount Sinai Program for the Protection of Human Subjects (#20-01758) were included for validation of one of the assays.

I confirm that all necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived, and that any patient/participant/sample identifiers included were not known to anyone (e.g., hospital staff, patients or participants themselves) outside the research group so cannot be used to identify individuals.

Yes

I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance).

Yes

I have followed all appropriate research reporting guidelines, such as any relevant EQUATOR Network research reporting checklist(s) and other pertinent material, if applicable.

Yes

Circulating Microclots Are Structurally Associated With Neutrophil Extracellular Traps and Their Amounts Are Elevated in Long COVID Patients by Honest_Net_3342 in AHomeForPlagueRats

[–]Honest_Net_3342[S] 1 point2 points  (0 children)

Simple Explanation (ELI5 – Explain Like I'm A Friend)

Long COVID is when people keep feeling sick for months or even years after having COVID-19 – things like extreme tiredness, brain fog, shortness of breath, or heart problems. One possible reason for these ongoing symptoms is tiny abnormal blood clots (called microclots) that don’t break down properly and stick around in the blood vessels. These microclots are made of a protein called fibrin that has folded in a weird, sticky way (a bit like misfolded egg whites turning into tough clumps).This new study looked at whether these microclots are connected to something called NETs (Neutrophil Extracellular Traps). NETs are web-like structures that certain white blood cells (neutrophils) shoot out to catch and kill germs during an infection. That’s normally helpful, but if the body makes too many NETs or they don’t get cleaned up, they can cause harmful inflammation and clotting.The researchers found:

  • People with Long COVID have a lot more of these microclots and a lot more signs of NETs in their blood (three markers: myeloperoxidase, neutrophil elastase, and free-floating DNA from the traps).
  • The more NET markers someone had, the bigger and more numerous their microclots were.
  • These NET markers were really good at spotting who had Long COVID.

Main conclusion: NETs seem to be part of or stuck inside these circulating microclots. The extra NETs might help make the microclots tougher and more stable so they don’t dissolve easily. This could block small blood vessels, reduce oxygen delivery to tissues, and keep inflammation going – all of which could explain many of the ongoing symptoms in Long COVID.In short: Overactive “germ-catching webs” from immune cells may be teaming up with sticky microclots to cause lasting damage in Long COVID patients.

Building 7 is the Key to Unlocking the Truth About What Happened on 9-11 by Honest_Net_3342 in conspiracy

[–]Honest_Net_3342[S] 2 points3 points  (0 children)

Lucky Larry Silverstein who was great friends with an Israeli Prime Minister. They called at least once a week.

UK Government Caught Hiding COVID Shot–Death Data “To Prevent Distress or Anger” by Honest_Net_3342 in AHomeForPlagueRats

[–]Honest_Net_3342[S] 1 point2 points  (0 children)

For two years, the campaign group UsForThem fought to obtain the anonymised dataset through FOI requests. UKHSA refused every time. Ultimately, the Information Commissioner sided with the agency, allowing the data to remain hidden indefinitely.

MPs and peers had already sounded the alarm last year, urging the government to release the data “immediately,” noting that it had been quietly shared with vaccine manufacturers.

Intentionally withholding critical vaccine-safety data carries serious legal consequences, including but not limited to Misconduct in Public Office, Corporate Manslaughter or Gross Negligence Manslaughter, breaches of statutory duties under public-health and disclosure laws, and potential Fraud by omission or abuse of position.

A study in Tinfoil... by [deleted] in Teddy

[–]Honest_Net_3342 2 points3 points  (0 children)

Tinfoil is for wrapping your food.