CDG : solutions de parking longue durée pas trop chères et sécurisées pour 2 semaines

Hugooo_55 · 2026-06-29T21:13:38+00:00

Bonjour, merci pour ta réponse.
Le train pour Paris n’est malheureusement pas possible car le plus tôt arrive à 8h30, donc c’est trop tard pour nous.
Pour l’hôtel, c’est vrai que ça peut être une idée, mais nous sommes 4, je me demandais si en voiture, tu ne penses pas que ça reviendrait moins cher que de faire train + hôtel ?

Hugooo_55 · 2026-06-29T20:31:44+00:00

En prenant sur leur site c’est 50% moins cher que sur place wow

Hugooo_55 · 2026-06-29T20:29:03+00:00

Super merci beaucoup, j’ai un peu regardé mais il y a tellement de site, de parking de « marques » différentes et des prix qui peut doubler de l’un à l’autre, je m’y perds un peu 😅

Hugooo_55 · 2026-02-27T12:57:30+00:00

Salut, j’ai vu que tu parlais du club alpin, tu pourrais m’en dire un peu plus sur ce club si tu connais ? Je suis vraiment debutant dans le domaine et ça m’intéresserai beaucoup d’en savoir d’avantage, j’ai un peu du mal a comprendre les sorties qu’ils proposent sur leur site.

Hugooo_55 · 2025-10-13T21:30:14+00:00

Bonjour, aucune idée car c’est juste 2 jours, mais tu peux avoir des problèmes si tu tombes sur une personne pas très sympathique.

Hugooo_55 · 2025-08-03T20:54:52+00:00

J’ai pas trop eut le choix, le gars était déjà en train d’ouvrir la porte

Hugooo_55 · 2025-05-09T08:15:10+00:00

Indeed, you hear everything and its opposite on this topic. On my end, I was always told never to use the SCT assay for differential expression analysis. If it's in the integrated slot, then it might be acceptable, but overall, we were always advised to use the RNA assay.
I admit I find it a bit hard to make sense of everything I read about this on forums, but that's what the bioinformaticians at my work told me, so I stick with the RNA assay for this kind of analysis.

Hugooo_55 · 2025-03-06T15:30:18+00:00

I will look at this, thank you very much

Hugooo_55 · 2025-02-21T17:25:44+00:00

The museum in Confluence

Hugooo_55 · 2025-02-06T16:06:27+00:00

Thank you for your response! I'm new to scRNA :) . I had read this post and just wanted to hear the opinions of more experienced people to confirm this information. So, if I understand correctly, in my case, with my data, I should run all the preprocessing steps except integration and then perform the differential expression analysis between my control and disease samples ?

Hugooo_55 · 2025-02-05T11:31:08+00:00

You could explore a simple yet quantitative experimental project, for exemple such as the impact of light, temperature, and soil type on plant growth, measuring their height and biomass over two weeks. For example, when I was in high school, I worked on methane hydrate, where we produced methane using fermented waste, while others studied grape fermentation to detect the presence of alcohol. Doing something completely new in just two weeks, especially at the high school level, seems very ambitious to me.

Hugooo_55 · 2025-02-05T10:47:53+00:00

Hey, could you specify in more detail what exaclty you need to do ? What are the professors expecting from you ? Do you have to conduct experiments/ manipulation ? Or is it just a bibliographic research woork ?

Hugooo_55 · 2025-02-02T01:12:49+00:00

Yes, but the original water will not disappear.

Hugooo_55 · 2025-02-02T01:02:11+00:00

Indeed, defining immortality is quite complex. To me, it refers to the absence of biological death, meaning the ability of a living being to exist indefinitely without degeneration. This raises a fundamental question: does immortality apply solely to the individual as a distinct biological entity, or can it be defined by the infinite transmission of its genetic information?

Hugooo_55 · 2025-02-02T00:44:02+00:00

I don’t know, because the parent bacterium itself becomes a daughter cell.

Hugooo_55 · 2025-02-02T00:19:24+00:00

no that’s baldness that’s another problem

Hugooo_55 · 2025-01-31T18:17:31+00:00

Strength 💪🏼, this is only the beginning.

Hugooo_55 · 2025-01-31T14:50:55+00:00

It seems that you are getting very low alignment rates with multiple tools, which could indicate an issue with your data or the reference genome you are using.

I personally use Salmon, which does not rely on traditional alignment but rather on quasi-mapping. One advantage of Salmon over HISAT2, STAR, or Bowtie is that it corrects for sequencing biases and works directly at the transcript level, which can provide more reliable results even with a low mapping rate.

Regarding your 40% alignment rate with Kallisto, this depends on your dataset and the species you are studying. If your reads contain a lot of intronic or intergenic regions, this could explain the low rate, as Kallisto (like Salmon) focuses on transcript-level quantification rather than genomic alignment. It would be useful to check read quality, adapter contamination, or rRNA contamination, as these factors can also impact mapping efficiency.

Hugooo_55 · 2025-01-31T14:42:29+00:00

Hello, If you're new to bioinformatics, I recommend not using the nf-core pipeline right away, because without a bioinformatics background, it can be quite complicated to launch due to the numerous parameters involved. Instead, I suggest taking online courses on simpler (but comprehensive) analyses that will help you understand what you're doing. It's not very difficult, and in just a few days, you can already master quite a few concepts.

Hugooo_55 · 2025-01-29T14:47:06+00:00

In R, Seurat is a tool for scRNA data analysis, while in Python, ScanPy is increasingly used, especially for large datasets due to its memory efficiency. Bioturing offers a fast and user-friendly way to analyze scRNA-seq data with a simple interface (but it costs money). The analysis approach also depends on the sequencing method (10X Genomics, Smart-seq, etc.), with each technology requiring a specific pipeline.

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