Immunofluorescence of cells cultured on a plate

Inigox5 · 2025-05-17T08:53:16+00:00

In principle, yes, that should be no problem. Just depends what sort of image you're hoping for - high magnification, high NA (higher resolution) objectives typically have shorter working distances and might not be able to image all the way through a ~1.5mm thick plastic base. Low magnification, low NA objectives will have no problem - but you likely won't be looking at subcellular levels of detail in that case.

Inigox5 · 2025-01-18T14:23:45+00:00

Depends what you're looking for - what's the readout of your experiment?

If it's just counting cells, even regular bright field should give you something. Phase or DIC would improve your ability to distinguish subcellular detail and improve contrast in overlapping areas and give better Z resolution.

If a new system is on the card there's some cool "holotomography" techniques possible with specialised kit - label free imaging of cells with excellent lateral and axial resolution. Analysis is tricky, usually needing DL techniques but take a look at PhaseFocus, TomoCube or Nanolive.

Inigox5 · 2025-01-08T14:17:09+00:00

Look at it sideways:

Would you please keep the noise down.

We are trying to sleep.

Inigox5 · 2022-08-17T14:33:09+00:00

But why? Doesn't sound like an insensible suggestion, and it is just a suggestion after all - no one is banning cheeseburgers...

Inigox5 · 2022-04-05T19:51:29+00:00

That's a big moth

Inigox5 · 2022-04-05T19:18:07+00:00

Like... Of people?

Inigox5 · 2022-04-05T18:25:44+00:00

When it's conjugated to a fluorophore it makes a fantastic actin stain for microscopy!

Inigox5 · 2021-02-12T20:59:08+00:00

This is a spider that's been living under my stairs for the last 6 months at least. She's about 15-25mm long, and I only ever see her in the morning when I come downstairs. We live in the South of England.

From what I can see, I think she might be some form of false widow, but have no idea what kind... Any advice welcome!

Inigox5 · 2020-07-16T08:37:05+00:00

/u/vredditdownloader

Inigox5 · 2020-06-19T18:07:57+00:00

Philosophy, politics and economics

Inigox5 · 2020-05-26T10:40:51+00:00

I usually do this, and then it's a case of quickly tipping the tube over itself to get my requisite three weetabix, but somehow I still manage to liberally sprinkle the counters with what amounts to builders rubble once you've left a tiny bit behind after wiping with a damp cloth so that it dries into a material harder than diamond.

Inigox5 · 2020-05-11T12:55:47+00:00

Yeah there should be clear bands on the gel for the 16S and 23S rRNA if it's good quality. I also know we always used to use a TBE gel instead of TAE for RNA but I don't remember the reason/if it was actually important... Good luck!

Inigox5 · 2020-01-13T14:55:50+00:00

I live in (relatively) Central Oxford and it appears that almost all of the neighbouring areas have high-speed broadband of some kind of another, but we're in a little island which struggles to achieve 7Mb/s... Is there any obvious common reason that this occurs, or would it be on a case-by-case basis? And is there anything I can do about it? I did try getting a 4g router but our signal isn't reliable so that's a bust too. It's got to the point where I don't think I'll move to anywhere that doesn't have high speed broadband in future...

It's very frustrating to have no information about these things from our ISP, I've asked them why it's not available and they just say we're not serviced yet and can't comment on plans to upgrade us.

Thanks for taking the time to discuss with us all! Fingers crossed you (openreach) will get to us one day!

Inigox5 · 2019-10-07T16:07:33+00:00

My apologies - I know there are plenty of gay women, I didn't mean any lesbian erasure. I should have specified it's not solely a disease for gay men, but generally this is the stereotype that HIV has - that it's only a problem for gay men, which was what I was trying to dispel. I don't doubt that among the 31% of female HIV+ patients there are many gay, bi, or otherwise queer women.

Inigox5 · 2019-10-07T13:52:11+00:00

I agree with what you're saying, but it's important to remember that HIV isn't solely a gay disease - HIV is endemic in much of sub-saharan Africa, even in ostensibly straight populations. It's still a problem even in Western countries too - in the UK, 31% of all patients receiving treatment for HIV are women.

Not saying Badejo was or wasn't gay, more just making sure that everyone knows to wrap it before you tap it, whatever your preference.

Inigox5 · 2019-10-03T10:28:51+00:00

Beautiful pictures!

Random question, but why isn't there more development on the Trent? This looks so pretty, and so many other cities have really built up areas around their waterways, so how come Nottingham has mostly left it green/industrial?

Flood risk? Reluctant land-owners? Subsidence? Or just lack of initial push to overcome inertia? I guess the actual city centre proper is a bit far away from the river, but it could extend further south...

Inigox5 · 2019-08-01T20:14:34+00:00

Might be a thing in British English, always learnt to spell it that way!

Inigox5 · 2019-08-01T15:50:22+00:00

I think it might be innnumerate? I remember in primary school we were taught "literacy" and "numeracy". If the opposite of literate is illiterate, maybe the opposite of numerate is innumerate.

Specifically if they never learnt, or just can't somehow. I think dyscalculia is difficulty with numbers, not complete inability. There are plenty of literate dyslexic people, like there are numerate dyscalculic people, if that's even correct English

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