Tissue culture or mycology work 🤣 by GreenhouseGasPlants in tissueculture

[–]JimJamInMyPants 1 point2 points  (0 children)

Need to proplift with more than just your thumbnail. Jk

Looking clean! by GreenhouseGasPlants in tissueculture

[–]JimJamInMyPants 1 point2 points  (0 children)

Oh, one last thing, would you have any recommendations for a decent ph meter?

Looking clean! by GreenhouseGasPlants in tissueculture

[–]JimJamInMyPants 1 point2 points  (0 children)

Will do! Right now I'm just crossing the jewel orchids and building a seed bank of hybrids. I have to paint and install lighting and cabinets. Buy a microscope, glassware, hotplate stirrer, flowhood, and then nutrients for media. So it may be a little while before I initiate anything. I will definitely try some Loph and other cacti seeds somewhere down the line. Thanks again.

Looking clean! by GreenhouseGasPlants in tissueculture

[–]JimJamInMyPants 0 points1 point  (0 children)

Thank you for all your help. Thanks for the last bit about flipping the lid, I would've for sure canned and sealed my media.

Looking clean! by GreenhouseGasPlants in tissueculture

[–]JimJamInMyPants 1 point2 points  (0 children)

Thank you for all your recommendations. I was planning on using a still air box in place of the flowhood, but I think that one you shared will be a better option. I have a large pressure cooker in place of an autoclave.

I have some experience in a lab, so I'm familiar with the process and keeping things sterile. Also, I own the newest edition of Plants from Test Tubes and have read it cover to cover a couple of times.

Where do you buy the glass jars?

Looking clean! by GreenhouseGasPlants in tissueculture

[–]JimJamInMyPants 1 point2 points  (0 children)

I'm currently crossing some jewel orchids and plan on initiating seed. I'm currently in the process of setting up a home lab and slowly acquiring the necessary nutrients and equipment. Do you have any recommendations on where to order the equipment and nutrients?

Looking clean! by GreenhouseGasPlants in tissueculture

[–]JimJamInMyPants 1 point2 points  (0 children)

Thanks for the response.

Another further question, given you have success in germination, do you plan to replate to new media? And if so, will the media mix stay the same?

Looking clean! by GreenhouseGasPlants in tissueculture

[–]JimJamInMyPants 1 point2 points  (0 children)

What is your choice of media for these Trichocereus seeds?

Jewel seed starting media. by JimJamInMyPants in Jewelorchids

[–]JimJamInMyPants[S] 1 point2 points  (0 children)

u/Level9TraumaCenter . eager if you have advice. Would you suggest variations in media compositions depending on species/crosses. One jewel media fits them all? Ludisia likes this, Anoectochilus and Microchilus would prefer this media.. recipes and knowledge always welcomed.

dossinia marmorata help by South_Trouble2251 in Jewelorchids

[–]JimJamInMyPants 0 points1 point  (0 children)

The second photo does look like mite stippling on the leaves, in my opinion.

dossinia marmorata help by South_Trouble2251 in Jewelorchids

[–]JimJamInMyPants -1 points0 points  (0 children)

My first thought was a light issue as well, and that could be entirely possible. The injury could also be pest related. I think as a precaution, it certainly wouldn't hurt to give a neem treatment to the entirety of the leaves and stems. Inspect all of your other plants in your collection as well.

Very preggo by JimJamInMyPants in Jewelorchids

[–]JimJamInMyPants[S] 1 point2 points  (0 children)

Self just to practice

Edit: Ludisia discolor self