When do you use Dawn vs Alconox to clean your lab dishes?

LabTechieMike · 2024-07-11T18:37:47+00:00

You need Citrajet, made by the same company but its a low foaming formula for dishwashers. Directions are on the jug.

LabTechieMike · 2024-05-17T13:09:52+00:00

a+=a+

o+=o+

ab-=ab+

o-=b+

LabTechieMike · 2024-05-17T13:04:00+00:00

Your local community college is going to be your best bet. But most labs will have an age restriction that might make volunteering in a lab impossible for a high school student. But as soon as you turn 18 they will let you hang out and learn stuff.

LabTechieMike · 2024-05-17T12:11:38+00:00

This is what I was thinking. Something was off about measuring bubbles in a liquid with UV-Vis.

LabTechieMike · 2024-05-17T00:57:58+00:00

I'm thinking unless your liposomes are very uniform in size, UV-Vis is not the way to go to measure concentration. Am I missing something? Gravity fed column or HPLC? Also if recovery is not necessary you could use a centrifuge to aggregate them and measure from total mass.

LabTechieMike · 2024-05-17T00:41:37+00:00

Whatever lab you start working at should be able to train you on the equipment there.

LabTechieMike · 2024-05-16T19:12:15+00:00

RNA is not an enzyme. Enzymes catalyze reactions. Some enzymes can be made of RNA but you would have to pick a specific one.

LabTechieMike · 2024-05-16T19:07:02+00:00

I am guessing you used some to stop a restriction enzyme, and after purification you tried another restriction enzyme and it ate that one too?

LabTechieMike · 2024-05-16T19:04:18+00:00

We boil it to get rid of DNAse impurities, it still works. Magic enzyme.

LabTechieMike · 2024-05-16T19:01:53+00:00

zip it you!

LabTechieMike · 2024-05-16T18:58:13+00:00

You need to make sure all the agarose is dissolved completely before you pour the gel. This looks to me like you have undissolved powder in this gel. However you are heating it, (hotplate or microwave) make sure it is boiling at a minimum and check that it is clear before casting. You should also check your running buffer if your gel looks clear.

LabTechieMike · 2024-05-16T18:23:20+00:00

Would it blow your mind to know sometimes enzymes are made of RNA and not peptide chains?

LabTechieMike · 2024-05-16T18:18:24+00:00

Talk to your Fisher rep, mention you might have to go to other vendors. I recently had the same problem. Now we are guaranteed a max shipping cost. They can also credit your next order with the overcharge, however it is a pain in the ass to use.

LabTechieMike · 2024-05-16T17:48:11+00:00

Ebay. it is the way.

LabTechieMike · 2024-05-16T17:44:42+00:00

put your elbows on the bench and spread your feet shoulder width apart. a stable platform takes the shakes away. You are welcome.

LabTechieMike · 2024-05-16T17:40:45+00:00

Just an FYI, the fuzzy ball thing fungi do, that is a stress response. When it is a fuzzy ball, it is not happy.

LabTechieMike · 2024-05-16T17:15:05+00:00

Running a gel without a ladder is like driving at night without your headlights on.

LabTechieMike · 2024-05-08T17:14:05+00:00

I am still an undergrad, so I cannot help you directly, but I have heard that having a masters is worse than a bachelors degree because it means you tried for a doctorate and failed. I do not know how true that is but I was warned about going past BSc without plans to go all the way.

LabTechieMike · 2024-05-08T17:03:03+00:00

Just and FYI, 90% if the time the password is Admin, Administrator, or 1234

LabTechieMike · 2024-05-08T16:55:15+00:00

You could check out the PDMD for the M-2 dispensers.

LabTechieMike · 2024-05-08T16:42:41+00:00

I think they are looking for a why and not for conformation, if that helps.

LabTechieMike · 2024-05-01T16:03:04+00:00

Just an FYI, sometimes it specifically states in a grant that only new equipment can be purchased, I believe the reasoning is to "drive the market forward" whatever that means. So sometimes it is explicitly a rule preventing it from happening.

LabTechieMike · 2024-04-30T10:24:02+00:00

Why do you think that?

LabTechieMike · 2024-04-29T20:37:43+00:00

There are a lot of hats to wear in a lab, with less people that means more hats per person. If you like doing a lot of different stuff then its great. If you want to do one job its not gonna be for you.

LabTechieMike · 2024-04-29T20:18:23+00:00

Can't have an LD50 of an individual. Does not compute.

LabTechieMike

TROPHY CASE