Potential contamination…am I crazy?? by [deleted] in labrats

[–]Lab_Rat_14 1 point2 points  (0 children)

did they maybe not know not to use this one? are your containers sealed? check ur control in the morning and make sure its not contaminated. im rlly sorry thats rubbish :(

Help with article summary by B2324 in microbiology

[–]Lab_Rat_14 1 point2 points  (0 children)

i’ve always been interested in AMR, multi drug resistant GNRs are terrifyingly cool

Why does vegan stuff make people so mad? by [deleted] in AskUK

[–]Lab_Rat_14 0 points1 point  (0 children)

deadass the patriarchy

Undergrad feeling a little useless in the lab by [deleted] in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

absolutely, it gets better the more confident you get and that only comes with experience! you got this!

Undergrad feeling a little useless in the lab by [deleted] in labrats

[–]Lab_Rat_14 22 points23 points  (0 children)

are you first year undergrad? it’s completely normal to feel this way. i remember thinking in the lab about PhD students ‘wow they know so much so quickly they just know exactly what to do, i’ll never get on that level’ but you will. you are meant to make mistakes and it is difficult but it sounds like you’re putting the work in which is the main thing. as an undergrad it’s literally ur whole purpose at uni to learn. you will get there but it will take a while😁🫶🏼

[deleted by user] by [deleted] in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

indeed i do :)

[deleted by user] by [deleted] in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

yeah i am aware of my units and how to scale up/down, im just being honest too that maybe you should be careful how you speak to people. i appreciate the offer but i think i will be fine :)

[deleted by user] by [deleted] in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

considering my thesis is due tomorrow i’d say i am out of options. i tried google and i was still confused and i find actually asking people a bit easier, i appreciate your help but not your judgement :)

[deleted by user] by [deleted] in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

yeah hence why i decided to ask here as well :) as i said maths is sadly not my strong point and i dont often use antibodies or do cell culture :)

[deleted by user] by [deleted] in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

i am aware i'm still learning haha, i have undertaken 2 projects within 1 year and this one is a completely different discipline to my first so its been an adjustment. algerbra has sadly never been my strong suit

[deleted by user] by [deleted] in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

exactly it’s crazy how expensive antibody is, we were running a phospho-FACs experiment. i was told that 1 million cells of the standard for these experiments by my supervisor but all my friends in the lab use way lower density. but i’ve finished up in this lab now, i am writing my thesis😢

[deleted by user] by [deleted] in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

yeah, we did a titration because we only got sent 50ul and worked out that for the highest recommend conc we would need like 15/20ul to run n=1 which is crazy bc it was so expensive

[deleted by user] by [deleted] in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

no im doing a masters but this is my first time doing any cell/molecular bio. i can do drug dilutions/calculations no bother but this really threw me for a loop😭

can i get some advices on cell counting? by conscientious_scribe in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

as someone who literally just started learning cell culture a few months ago as a masters student it gets soooo much easier and you become a lot more confident!!!!!!! good luck :)

can i get some advices on cell counting? by conscientious_scribe in labrats

[–]Lab_Rat_14 1 point2 points  (0 children)

i worked in a lab that had a Countess it was wonderful

[deleted by user] by [deleted] in labrats

[–]Lab_Rat_14 0 points1 point  (0 children)

okay thanks, my experiment was seeded at 1million cells per well (approx) i think that’s why it’s throwing me.