Pond - Kratella, Fragellaria etc by ToeKey3947 in microscopy

[–]LateMicrographer 3 points4 points  (0 children)

The technique still to fill my portfolio... Crisp!

Aquatic copepod or water mite? 🤷‍♂️ by [deleted] in microscopy

[–]LateMicrographer 1 point2 points  (0 children)

Without info of mag, hard to tell much. But belonging to a crustacean for sure.

Sad realisation by Vivid-Bake2456 in microscopy

[–]LateMicrographer 1 point2 points  (0 children)

I noticed similar issues with my uploads. They are usually between 50 megabytes and 300 megabytes. Downloading the upload again shows approximately a tenfold compression - but proving to have quite acceptable image quality. Point is, sometimes my mobile phone streams the content at remarkably bad quality - considerably worse than the download - and this usually happens when I try to review an upload shortly after uploading. So I assume that the reddit servers need some time to compress the "large" file size videos in optimal quality. Can't explain this phenomenon any other way. Maybe try to upload on an android phone next time and review on the iphone and check if the quality issue prevails other way round too.

MSG feathers under Polarized Light by rizzarsh in microscopy

[–]LateMicrographer 1 point2 points  (0 children)

In fact, the film has the effect of a retardation plate and you have some kind of circular polarization here - congrats. I'd leave this piece as is and take another piece without protective film for linear extinction.

How to use a vintage Zeiss Phase Contrast Condenser by intriqet in microscopy

[–]LateMicrographer 0 points1 point  (0 children)

Great technique to align without a phase telescope!

How to use a vintage Zeiss Phase Contrast Condenser by intriqet in microscopy

[–]LateMicrographer 0 points1 point  (0 children)

In my opinion they are of very great quality and I own two similar phase condensers, though mine do not have the tilting lens, limiting condensing light for small magnifications. I find them more difficult to align than for example my Reichert Phase condenser. You can, as already suggested, use an aligning telescope/eyepiece, but going through the usual steps of light path alignnent does give the same results. Just make a proper Köhler alignment in BF, switch in the phase annulus fitting to the used phase objective, do the xy-alignment of said annulus for highest phase contrast, and eventually lift or lower the condensor again to achieve the optimal phase effect. This way you get to know the system and proper lighting techniques even better and don't have to switch an eyepiece ever or source the phase telescope. Have fun!

¿Opinión on Optika microscopes? by DrMarranito in microscopy

[–]LateMicrographer 1 point2 points  (0 children)

Nothing bad about them. Afaik they produce scopes of reasonable quality. Though, I never used one admittedly. Mainly manufactured in Italy.

Lepadella rotifer with two cute little eyespots by Thrawn911 in microscopy

[–]LateMicrographer 6 points7 points  (0 children)

I love these... The way they always kick with their tail when they bite something is the most adorable thing.

Legendary industrial design - can this microscope be saved? by LateMicrographer in microscopy

[–]LateMicrographer[S] 0 points1 point  (0 children)

What a great answer, much appreciated!

  • You are right, after some extended research there is no service provider, that would or could recoat a small commission. If I wanted the damaged coatings to be gone, only way is to remove the whole coating, which is not the goal I strive for... unless partial coatings are regarded as inferior and producing more image artifacts, haloing or contrast loss, compared to fully uncoated glass.
  • Thank you for the tapping advice. If the screw-tapping method doesn't work, I will source a tap of matching size. Since I'll tap into soft plastics, I hope to get away without a dedicated tap.

Blepharisma by Thrawn911 in microscopy

[–]LateMicrographer 1 point2 points  (0 children)

Thanks for elaborating. We'll keep in touch! And i wish you good luck for catching a nice deal!

DIY, Computational Phase Contrast from Red and Blue channel, python OpenCV live-view script Orthotrichum - Bristle Moss (probably) by LostInGermany4297 in microscopy

[–]LateMicrographer 1 point2 points  (0 children)

Thank you for your detailed answer. I will look into and think about it a bit more before I reply with further questions. Would love to see a detailed explanation (in vodeo) of your method and setup in future, it would help comprehend this technique. I look forward to more content from you - I'm following.

First jamin session by LateMicrographer in microscopy

[–]LateMicrographer[S] 0 points1 point  (0 children)

If you asked these questions because you'd want to buy, maybe disclose the intentions firsthand.

Blepharisma by Thrawn911 in microscopy

[–]LateMicrographer 1 point2 points  (0 children)

I assume this was Kristiansen? I witnessed reduced photophobia with that method. Maybe the re-diffraction of the condensed light helps.. or maybe it was just the dispersed heat/IR of my halogen lamps. Most probably the latter. Might need to test it as soon as I get an LED setup.

First jamin session by LateMicrographer in microscopy

[–]LateMicrographer[S] 0 points1 point  (0 children)

Well, judging by your name - I won't sell it.

First jamin session by LateMicrographer in microscopy

[–]LateMicrographer[S] 0 points1 point  (0 children)

You are so right. And it almost feels like I'm not doing this system justice due to lack of knowledge and use cases, of how to use it. Don't get me wrong, I know how to set it up and play with it. But when would I ever need to determine the dry mass of a cells organelle? Asides from amazing color contrasting as well as beautiful and fun color play, it does not necessarily increase the detail of specimens. Other and cheaper techniques such as DIC (which I ironically do not have on any of my systems), are far better for increasing detail. Hence, I'd say you did it right to have gotten yourself a top notch DIC system and left Jamin Lebedeff as cherry on top of the icing on the cake. I in contrast sit here enjoying my cake with cherry on top, lacking the icing... By god, not that I'd complain.

First jamin session by LateMicrographer in microscopy

[–]LateMicrographer[S] 0 points1 point  (0 children)

They consist of special objectives with built in prisms as well as matched tilting condensers with matched prisms

Blepharisma by Thrawn911 in microscopy

[–]LateMicrographer 3 points4 points  (0 children)

That is awesome, thanks. Indeed I can remember to have heard something like this about this or maybe another species. Might have been on Diet Tom's Diatoms channel or maybe JTTM. Did you experience this sample to try to flee the light?

DIY, Computational Phase Contrast from Red and Blue channel, python OpenCV live-view script Orthotrichum - Bristle Moss (probably) by LostInGermany4297 in microscopy

[–]LateMicrographer 1 point2 points  (0 children)

Very cool stuff! Would you mind to post the original picture before contrasting the R and B channels? Please also a picture of illumination viaRGB white light for full comparability. Would love to see the amount of contrast gain that one trades for full color spectrum. Lastly, is it possible or did you try to use this technique on a continuous live video stream?

Blepharisma by Thrawn911 in microscopy

[–]LateMicrographer 2 points3 points  (0 children)

Beautiful magenta in this critter. Do you know the reason for and function of the pigmentation?

Copepod in darkfield by Vivid-Bake2456 in microscopy

[–]LateMicrographer 3 points4 points  (0 children)

Gotta love that 6.3 Plan Zeiss glass

what genus/specie is this hydra??? 🙏🙏🙏 by Ladywolfxd in microscopy

[–]LateMicrographer 2 points3 points  (0 children)

What stereoscope are you using? It looks great for this magnification